Details
| Original language | English |
|---|---|
| Pages (from-to) | 16050-16061 |
| Number of pages | 12 |
| Journal | Environmental Science and Pollution Research |
| Volume | 24 |
| Issue number | 19 |
| Publication status | Published - 23 May 2017 |
Abstract
Plutonium associated with higher molecular weight molecules is presumed to be poorly mobile and hardly plant available. In our present study, we investigate the uptake and effects of Pu treatments on Solanum tuberosum plants in amended Hoagland medium at concentrations of [242Pu] = 100 and 500 nm, respectively. We found a direct proof of oxidative stress in the plants caused by these rather low concentrations. For the confirmation of oxidative stress, we explored the production of nitric oxide (NO) and hydrogen peroxide (H2O2) by epifluorescence microscopy. Oxidative stress markers like lipid peroxidation and superoxide radicals (O2 •−) are monitored through histochemical analysis. The biochemical parameters i.e. chlorophyll and carotenoids are measured as an indicator of cellular damage in the tested plants including the enzymatic parameters such as catalase and glutathione reductase. From our work, we conclude that Pu in low concentration has no significant effects on the uptake of many trace and macroelements. In contrast, the content of O2 •−, malondialdehyde (MDA), and H2O2 increases with increasing Pu concentration in the solution, while the opposite effects was found for NO, catalase, and glutathione reductase. These findings prove that even low concentration of Pu regulates ROS production and generate oxidative stress in S. tuberosum L.
Keywords
- Catalase, Glutathione reductase, Hydrogen peroxide, Lipid peroxidation, Macro/microelements, Nitric oxide, Plutonium
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
- Environmental Science(all)
- Pollution
- Environmental Science(all)
- Health, Toxicology and Mutagenesis
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In: Environmental Science and Pollution Research, Vol. 24, No. 19, 23.05.2017, p. 16050-16061.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Investigation of low-level 242Pu contamination on nutrition disturbance and oxidative stress in Solanum tuberosum L
AU - Gupta, Dharmendra K.
AU - Tawussi, Frank
AU - Hölzer, Alex
AU - Hamann, Linda
AU - Walther, Clemens
N1 - Funding information: We acknowledge financial support from the BMBF for FT (funding 02S9082A) and DG (funding 02S9276D). AH thanks the Sasse foundation for their continuous support.
PY - 2017/5/23
Y1 - 2017/5/23
N2 - Plutonium associated with higher molecular weight molecules is presumed to be poorly mobile and hardly plant available. In our present study, we investigate the uptake and effects of Pu treatments on Solanum tuberosum plants in amended Hoagland medium at concentrations of [242Pu] = 100 and 500 nm, respectively. We found a direct proof of oxidative stress in the plants caused by these rather low concentrations. For the confirmation of oxidative stress, we explored the production of nitric oxide (NO) and hydrogen peroxide (H2O2) by epifluorescence microscopy. Oxidative stress markers like lipid peroxidation and superoxide radicals (O2 •−) are monitored through histochemical analysis. The biochemical parameters i.e. chlorophyll and carotenoids are measured as an indicator of cellular damage in the tested plants including the enzymatic parameters such as catalase and glutathione reductase. From our work, we conclude that Pu in low concentration has no significant effects on the uptake of many trace and macroelements. In contrast, the content of O2 •−, malondialdehyde (MDA), and H2O2 increases with increasing Pu concentration in the solution, while the opposite effects was found for NO, catalase, and glutathione reductase. These findings prove that even low concentration of Pu regulates ROS production and generate oxidative stress in S. tuberosum L.
AB - Plutonium associated with higher molecular weight molecules is presumed to be poorly mobile and hardly plant available. In our present study, we investigate the uptake and effects of Pu treatments on Solanum tuberosum plants in amended Hoagland medium at concentrations of [242Pu] = 100 and 500 nm, respectively. We found a direct proof of oxidative stress in the plants caused by these rather low concentrations. For the confirmation of oxidative stress, we explored the production of nitric oxide (NO) and hydrogen peroxide (H2O2) by epifluorescence microscopy. Oxidative stress markers like lipid peroxidation and superoxide radicals (O2 •−) are monitored through histochemical analysis. The biochemical parameters i.e. chlorophyll and carotenoids are measured as an indicator of cellular damage in the tested plants including the enzymatic parameters such as catalase and glutathione reductase. From our work, we conclude that Pu in low concentration has no significant effects on the uptake of many trace and macroelements. In contrast, the content of O2 •−, malondialdehyde (MDA), and H2O2 increases with increasing Pu concentration in the solution, while the opposite effects was found for NO, catalase, and glutathione reductase. These findings prove that even low concentration of Pu regulates ROS production and generate oxidative stress in S. tuberosum L.
KW - Catalase
KW - Glutathione reductase
KW - Hydrogen peroxide
KW - Lipid peroxidation
KW - Macro/microelements
KW - Nitric oxide
KW - Plutonium
UR - http://www.scopus.com/inward/record.url?scp=85019889215&partnerID=8YFLogxK
U2 - 10.1007/s11356-017-9071-9
DO - 10.1007/s11356-017-9071-9
M3 - Article
C2 - 28537023
AN - SCOPUS:85019889215
VL - 24
SP - 16050
EP - 16061
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
SN - 0944-1344
IS - 19
ER -