Details
| Original language | English |
|---|---|
| Pages (from-to) | 27369-27376 |
| Number of pages | 8 |
| Journal | RSC Advances |
| Volume | 10 |
| Issue number | 46 |
| Publication status | Published - 21 Jul 2020 |
Abstract
Sch-642305 is an unusual bicyclic 10-membered macrolide produced by the filamentous fungus Phomopsis sp. CMU-LMA for which no biosynthetic evidence exists. Here, we generate a draft genome sequence of the producing organism and discover the biosynthetic gene cluster responsible for formation of Sch-642305. Targeted gene disruptions together with reconstitution of the pathway in the heterologous host Aspergillus oryzae dissect key chemical steps and shed light on a series of oxidoreductions occuring in the pathway.
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Chemical Engineering(all)
- General Chemical Engineering
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In: RSC Advances, Vol. 10, No. 46, 21.07.2020, p. 27369-27376.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA
AU - Trenti, Francesco
AU - Lebe, Karen E.
AU - Adelin, Emilie
AU - Ouazzani, Jamal
AU - Schotte, Carsten
AU - Cox, Russell J.
N1 - Funding Information: FT thanks the HSBDR Doctoral Training Centre for funding. KEL and CS thank the Leibniz Universität Hannover for funding. Christopher Lambert is thanked for technical assistance. Genome data assembly were performed by CeBiTeC at the University of Bielefeld, and Prof. J. Kalinowski and Dr. D. Wibberg are thanked for data assembly work. DFG is thanked for the provision of NMR equipment (INST 187/686-1). J. O. thanks the IMAGIF High throughput sequencing platform at CNRS, Gif-sur-Yvette. The publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2020/7/21
Y1 - 2020/7/21
N2 - Sch-642305 is an unusual bicyclic 10-membered macrolide produced by the filamentous fungus Phomopsis sp. CMU-LMA for which no biosynthetic evidence exists. Here, we generate a draft genome sequence of the producing organism and discover the biosynthetic gene cluster responsible for formation of Sch-642305. Targeted gene disruptions together with reconstitution of the pathway in the heterologous host Aspergillus oryzae dissect key chemical steps and shed light on a series of oxidoreductions occuring in the pathway.
AB - Sch-642305 is an unusual bicyclic 10-membered macrolide produced by the filamentous fungus Phomopsis sp. CMU-LMA for which no biosynthetic evidence exists. Here, we generate a draft genome sequence of the producing organism and discover the biosynthetic gene cluster responsible for formation of Sch-642305. Targeted gene disruptions together with reconstitution of the pathway in the heterologous host Aspergillus oryzae dissect key chemical steps and shed light on a series of oxidoreductions occuring in the pathway.
UR - http://www.scopus.com/inward/record.url?scp=85089303743&partnerID=8YFLogxK
U2 - 10.1039/d0ra05311b
DO - 10.1039/d0ra05311b
M3 - Article
VL - 10
SP - 27369
EP - 27376
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 46
ER -