Details
| Original language | English |
|---|---|
| Pages (from-to) | 134-143 |
| Number of pages | 10 |
| Journal | New Phytologist |
| Volume | 200 |
| Issue number | 1 |
| Early online date | 26 Aug 2013 |
| Publication status | Published - Oct 2013 |
Abstract
This paper investigates the effects of cuticular wax on the release of strain and on the tensile properties of enzymatically isolated cuticular membranes (CMs) taken from leaves of agave (Agave americana), bush lily (Clivia miniata), holly (Ilex aquifolium), and ivy (Hedera helix) and from fruit of apple (Malus × domestica), pear (Pyrus communis), and tomato (Lycopersicon esculentum). Biaxial strain release was quantified as the decrease in CM disc area following wax extraction. Stiffness, maximum strain and maximum force were determined in uniaxial tensile tests using strips of CM and dewaxed CMs (DCMs). Biaxial strain release, stiffness, and maximum strain, but not maximum force, were linearly related to the amount of wax extracted. Apple CM has the most wax and here the effect of wax extraction was substantially accounted for by the embedded cuticular wax. Heating apple CM to 80°C melted some wax constituents and produced an effect similar to, but smaller than, that resulting from wax extraction. Our results indicate that wax 'fixes' strain, effectively converting reversible elastic into irreversible plastic strain. A consequence of 'fixation' is increased cuticular stiffness.
Keywords
- Crystallinity, Cuticle, Fracture, Rheology, Stiffness, Stress
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: New Phytologist, Vol. 200, No. 1, 10.2013, p. 134-143.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Intracuticular wax fixes and restricts strain in leaf and fruit cuticles
AU - Khanal, Bishnu Prasad
AU - Grimm, Eckhard
AU - Finger, Sebastian
AU - Blume, Alfred
AU - Knoche, Moritz
PY - 2013/10
Y1 - 2013/10
N2 - This paper investigates the effects of cuticular wax on the release of strain and on the tensile properties of enzymatically isolated cuticular membranes (CMs) taken from leaves of agave (Agave americana), bush lily (Clivia miniata), holly (Ilex aquifolium), and ivy (Hedera helix) and from fruit of apple (Malus × domestica), pear (Pyrus communis), and tomato (Lycopersicon esculentum). Biaxial strain release was quantified as the decrease in CM disc area following wax extraction. Stiffness, maximum strain and maximum force were determined in uniaxial tensile tests using strips of CM and dewaxed CMs (DCMs). Biaxial strain release, stiffness, and maximum strain, but not maximum force, were linearly related to the amount of wax extracted. Apple CM has the most wax and here the effect of wax extraction was substantially accounted for by the embedded cuticular wax. Heating apple CM to 80°C melted some wax constituents and produced an effect similar to, but smaller than, that resulting from wax extraction. Our results indicate that wax 'fixes' strain, effectively converting reversible elastic into irreversible plastic strain. A consequence of 'fixation' is increased cuticular stiffness.
AB - This paper investigates the effects of cuticular wax on the release of strain and on the tensile properties of enzymatically isolated cuticular membranes (CMs) taken from leaves of agave (Agave americana), bush lily (Clivia miniata), holly (Ilex aquifolium), and ivy (Hedera helix) and from fruit of apple (Malus × domestica), pear (Pyrus communis), and tomato (Lycopersicon esculentum). Biaxial strain release was quantified as the decrease in CM disc area following wax extraction. Stiffness, maximum strain and maximum force were determined in uniaxial tensile tests using strips of CM and dewaxed CMs (DCMs). Biaxial strain release, stiffness, and maximum strain, but not maximum force, were linearly related to the amount of wax extracted. Apple CM has the most wax and here the effect of wax extraction was substantially accounted for by the embedded cuticular wax. Heating apple CM to 80°C melted some wax constituents and produced an effect similar to, but smaller than, that resulting from wax extraction. Our results indicate that wax 'fixes' strain, effectively converting reversible elastic into irreversible plastic strain. A consequence of 'fixation' is increased cuticular stiffness.
KW - Crystallinity
KW - Cuticle
KW - Fracture
KW - Rheology
KW - Stiffness
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=84883052347&partnerID=8YFLogxK
U2 - 10.1111/nph.12355
DO - 10.1111/nph.12355
M3 - Article
C2 - 23750808
AN - SCOPUS:84883052347
VL - 200
SP - 134
EP - 143
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 1
ER -