Details
| Original language | English |
|---|---|
| Pages (from-to) | 1723-1731 |
| Number of pages | 9 |
| Journal | Journal of Biophotonics |
| Volume | 10 |
| Issue number | 12 |
| Publication status | Published - 2 May 2017 |
Abstract
Micro-/nanosphere-mediated femtosecond laser cell perforation is one of the high throughput technologies used for macro-molecule-delivery into multiple cells. We have demonstrated the delivery of plasmid-DNA/liposome complexes into cells using biodegradable polymer microspheres and a femtosecond laser and investigated the intracellular localization of the complexes by delivering fluorescence-labeled plasmid-DNA/liposome complexes into cells. The utilization of liposomes increases the number of complexes delivered into the cytoplasm by laser illumination, which contributed to the increased transfection rate. In the experiment involving polystyrene (PS) microspheres of different diameters, the fluorescence of the complexes was detected in the nucleus as well as cytoplasm after laser illumination for PS microspheres of 3.0 μm diameter. The direct delivery of complexes into the nucleus is probably attributed to the enhancement of the nuclear membrane permeability by the enhanced optical field obtained close to the nucleus. These revelations on the intracellular localization of foreign DNA would provide effective laser-based transfection. Picture: Intranuclear delivery of plasmid-DNA/liposome complexes by utilizing dielectric microspheres and a femtosecond laser.
Keywords
- biodegradable polymer, femtosecond laser, gene delivery, intracellular delivery, laser cell perforation
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- General Materials Science
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Engineering(all)
- General Engineering
- Physics and Astronomy(all)
- General Physics and Astronomy
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In: Journal of Biophotonics, Vol. 10, No. 12, 02.05.2017, p. 1723-1731.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Intracellular localization and delivery of plasmid DNA by biodegradable microsphere-mediated femtosecond laser optoporation
AU - Ishii, Atsuhiro
AU - Hiruta, Yuki
AU - Heinemann, Dag
AU - Heisterkamp, Alexander
AU - Kanazawa, Hideko
AU - Terakawa, Mitsuhiro
PY - 2017/5/2
Y1 - 2017/5/2
N2 - Micro-/nanosphere-mediated femtosecond laser cell perforation is one of the high throughput technologies used for macro-molecule-delivery into multiple cells. We have demonstrated the delivery of plasmid-DNA/liposome complexes into cells using biodegradable polymer microspheres and a femtosecond laser and investigated the intracellular localization of the complexes by delivering fluorescence-labeled plasmid-DNA/liposome complexes into cells. The utilization of liposomes increases the number of complexes delivered into the cytoplasm by laser illumination, which contributed to the increased transfection rate. In the experiment involving polystyrene (PS) microspheres of different diameters, the fluorescence of the complexes was detected in the nucleus as well as cytoplasm after laser illumination for PS microspheres of 3.0 μm diameter. The direct delivery of complexes into the nucleus is probably attributed to the enhancement of the nuclear membrane permeability by the enhanced optical field obtained close to the nucleus. These revelations on the intracellular localization of foreign DNA would provide effective laser-based transfection. Picture: Intranuclear delivery of plasmid-DNA/liposome complexes by utilizing dielectric microspheres and a femtosecond laser.
AB - Micro-/nanosphere-mediated femtosecond laser cell perforation is one of the high throughput technologies used for macro-molecule-delivery into multiple cells. We have demonstrated the delivery of plasmid-DNA/liposome complexes into cells using biodegradable polymer microspheres and a femtosecond laser and investigated the intracellular localization of the complexes by delivering fluorescence-labeled plasmid-DNA/liposome complexes into cells. The utilization of liposomes increases the number of complexes delivered into the cytoplasm by laser illumination, which contributed to the increased transfection rate. In the experiment involving polystyrene (PS) microspheres of different diameters, the fluorescence of the complexes was detected in the nucleus as well as cytoplasm after laser illumination for PS microspheres of 3.0 μm diameter. The direct delivery of complexes into the nucleus is probably attributed to the enhancement of the nuclear membrane permeability by the enhanced optical field obtained close to the nucleus. These revelations on the intracellular localization of foreign DNA would provide effective laser-based transfection. Picture: Intranuclear delivery of plasmid-DNA/liposome complexes by utilizing dielectric microspheres and a femtosecond laser.
KW - biodegradable polymer
KW - femtosecond laser
KW - gene delivery
KW - intracellular delivery
KW - laser cell perforation
UR - http://www.scopus.com/inward/record.url?scp=85018984534&partnerID=8YFLogxK
U2 - 10.1002/jbio.201600323
DO - 10.1002/jbio.201600323
M3 - Article
C2 - 28464530
AN - SCOPUS:85018984534
VL - 10
SP - 1723
EP - 1731
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 12
ER -