Details
| Original language | English |
|---|---|
| Pages (from-to) | 5129-5134 |
| Number of pages | 6 |
| Journal | ACS Biomaterials Science and Engineering |
| Volume | 7 |
| Issue number | 11 |
| Early online date | 4 Oct 2021 |
| Publication status | Published - 8 Nov 2021 |
Abstract
There is a great need in the biomedical field to efficiently, and cost-effectively, deliver membrane-impermeable molecules into the cellular cytoplasm. However, the cell membrane is a selectively permeable barrier, and large molecules often cannot pass through the phospholipid bilayer. We show that nanosecond laser-activated polymer surfaces of commercial polyvinyl tape and black polystyrene Petri dishes can transiently permeabilize cells for high-throughput, diverse cargo delivery of sizes of up to 150 kDa. The polymer surfaces are biocompatible and support normal cell growth of adherent cells. We determine the optimal irradiation conditions for poration, influx of fluorescent molecules into the cell, and post-treatment viability of the cells. The simple and low-cost substrates we use have no thin-metal structures, do not require cleanroom fabrication, and provide spatial selectivity and scalability for biomedical applications.
Keywords
- carbon black, intracellular delivery, metal oxide, polymer, pulsed laser, spatially selective delivery
ASJC Scopus subject areas
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
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In: ACS Biomaterials Science and Engineering, Vol. 7, No. 11, 08.11.2021, p. 5129-5134.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Intracellular Cargo Delivery Induced by Irradiating Polymer Substrates with Nanosecond-Pulsed Lasers
AU - Shen, Weilu
AU - Kalies, Stefan
AU - Madrid, Marinna
AU - Heisterkamp, Alexander
AU - Mazur, Eric
N1 - Funding Information: The research described in this paper was supported by National Science Foundation under contracts PHY-1219334 and PHY-1806434. This work was performed in part at the Harvard University Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Coordinated Infrastructure Network (NNCI), which is supported by the National Science Foundation under NSF award numbers ECCS-1541959 and ECCS-2025158. W.S. was funded by the Smith Family Graduate Science and Engineering Fellowship at Harvard University.
PY - 2021/11/8
Y1 - 2021/11/8
N2 - There is a great need in the biomedical field to efficiently, and cost-effectively, deliver membrane-impermeable molecules into the cellular cytoplasm. However, the cell membrane is a selectively permeable barrier, and large molecules often cannot pass through the phospholipid bilayer. We show that nanosecond laser-activated polymer surfaces of commercial polyvinyl tape and black polystyrene Petri dishes can transiently permeabilize cells for high-throughput, diverse cargo delivery of sizes of up to 150 kDa. The polymer surfaces are biocompatible and support normal cell growth of adherent cells. We determine the optimal irradiation conditions for poration, influx of fluorescent molecules into the cell, and post-treatment viability of the cells. The simple and low-cost substrates we use have no thin-metal structures, do not require cleanroom fabrication, and provide spatial selectivity and scalability for biomedical applications.
AB - There is a great need in the biomedical field to efficiently, and cost-effectively, deliver membrane-impermeable molecules into the cellular cytoplasm. However, the cell membrane is a selectively permeable barrier, and large molecules often cannot pass through the phospholipid bilayer. We show that nanosecond laser-activated polymer surfaces of commercial polyvinyl tape and black polystyrene Petri dishes can transiently permeabilize cells for high-throughput, diverse cargo delivery of sizes of up to 150 kDa. The polymer surfaces are biocompatible and support normal cell growth of adherent cells. We determine the optimal irradiation conditions for poration, influx of fluorescent molecules into the cell, and post-treatment viability of the cells. The simple and low-cost substrates we use have no thin-metal structures, do not require cleanroom fabrication, and provide spatial selectivity and scalability for biomedical applications.
KW - carbon black
KW - intracellular delivery
KW - metal oxide
KW - polymer
KW - pulsed laser
KW - spatially selective delivery
UR - http://www.scopus.com/inward/record.url?scp=85117316241&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.1c00656
DO - 10.1021/acsbiomaterials.1c00656
M3 - Article
C2 - 34606721
AN - SCOPUS:85117316241
VL - 7
SP - 5129
EP - 5134
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
SN - 2373-9878
IS - 11
ER -