Details
Original language | English |
---|---|
Pages (from-to) | 797-810 |
Number of pages | 14 |
Journal | PLANT CELL |
Volume | 22 |
Issue number | 3 |
Publication status | Published - Mar 2010 |
Abstract
The NADH dehydrogenase complex (complex I) of the respiratory chain has unique features in plants. It is the main entrance site for electrons into the respiratory electron transfer chain, has a role in maintaining the redox balance of the entire plant cell and additionally comprises enzymatic side activities essential for other metabolic pathways. Here, we present a proteomic investigation to elucidate its internal structure. Arabidopsis thaliana complex I was purified by a gentle biochemical procedure that includes a cytochrome c-mediated depletion of other respiratory protein complexes. To examine its internal subunit arrangement, isolated complex I was dissected into subcomplexes. Controlled disassembly of the holo complex (1000 kD) by low-concentration SDS treatment produced 10 subcomplexes of 550, 450, 370, 270, 240, 210, 160,140,140, and 85 kD. Systematic analyses of subunit composition by mass spectrometry gave insights into subunit arrangement within complex I. Overall, Arabidopsis complex I includes at least 49 subunits, 17 of which are unique to plants. Subunits form subcomplexes analogous to the known functional modules of complex I from heterotrophic eukaryotes (the so-called N-, Q-, and P-modules), but also additional modules, most notably an 85-kD domain including g-type carbonic anhydrases. Based on topological information for many of its subunits, we present a model of the internal architecture of plant complex I.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: PLANT CELL, Vol. 22, No. 3, 03.2010, p. 797-810.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Internal Architecture of Mitochondrial Complex I from Arabidopsis thaliana
AU - Klodmann, Jennifer
AU - Sunderhaus, Stephanie
AU - Nimtz, Manfred
AU - Jänsch, Lothar
AU - Braun, Hans Peter
PY - 2010/3
Y1 - 2010/3
N2 - The NADH dehydrogenase complex (complex I) of the respiratory chain has unique features in plants. It is the main entrance site for electrons into the respiratory electron transfer chain, has a role in maintaining the redox balance of the entire plant cell and additionally comprises enzymatic side activities essential for other metabolic pathways. Here, we present a proteomic investigation to elucidate its internal structure. Arabidopsis thaliana complex I was purified by a gentle biochemical procedure that includes a cytochrome c-mediated depletion of other respiratory protein complexes. To examine its internal subunit arrangement, isolated complex I was dissected into subcomplexes. Controlled disassembly of the holo complex (1000 kD) by low-concentration SDS treatment produced 10 subcomplexes of 550, 450, 370, 270, 240, 210, 160,140,140, and 85 kD. Systematic analyses of subunit composition by mass spectrometry gave insights into subunit arrangement within complex I. Overall, Arabidopsis complex I includes at least 49 subunits, 17 of which are unique to plants. Subunits form subcomplexes analogous to the known functional modules of complex I from heterotrophic eukaryotes (the so-called N-, Q-, and P-modules), but also additional modules, most notably an 85-kD domain including g-type carbonic anhydrases. Based on topological information for many of its subunits, we present a model of the internal architecture of plant complex I.
AB - The NADH dehydrogenase complex (complex I) of the respiratory chain has unique features in plants. It is the main entrance site for electrons into the respiratory electron transfer chain, has a role in maintaining the redox balance of the entire plant cell and additionally comprises enzymatic side activities essential for other metabolic pathways. Here, we present a proteomic investigation to elucidate its internal structure. Arabidopsis thaliana complex I was purified by a gentle biochemical procedure that includes a cytochrome c-mediated depletion of other respiratory protein complexes. To examine its internal subunit arrangement, isolated complex I was dissected into subcomplexes. Controlled disassembly of the holo complex (1000 kD) by low-concentration SDS treatment produced 10 subcomplexes of 550, 450, 370, 270, 240, 210, 160,140,140, and 85 kD. Systematic analyses of subunit composition by mass spectrometry gave insights into subunit arrangement within complex I. Overall, Arabidopsis complex I includes at least 49 subunits, 17 of which are unique to plants. Subunits form subcomplexes analogous to the known functional modules of complex I from heterotrophic eukaryotes (the so-called N-, Q-, and P-modules), but also additional modules, most notably an 85-kD domain including g-type carbonic anhydrases. Based on topological information for many of its subunits, we present a model of the internal architecture of plant complex I.
UR - http://www.scopus.com/inward/record.url?scp=77953226426&partnerID=8YFLogxK
U2 - 10.1105/tpc.109.073726
DO - 10.1105/tpc.109.073726
M3 - Article
C2 - 20197505
AN - SCOPUS:77953226426
VL - 22
SP - 797
EP - 810
JO - PLANT CELL
JF - PLANT CELL
SN - 1040-4651
IS - 3
ER -