Details
| Original language | English |
|---|---|
| Pages (from-to) | 299-316 |
| Number of pages | 18 |
| Journal | Plant molecular biology |
| Volume | 99 |
| Issue number | 4-5 |
| Early online date | 31 Jan 2019 |
| Publication status | Published - 1 Mar 2019 |
Abstract
KEY MESSAGE: Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification for powdery mildew. Plant pathogenic fungi successfully colonize their hosts by manipulating the host defense mechanisms, which is accompanied by major transcriptome changes in the host. To characterize compatible plant pathogen interactions at early stages of infection by the obligate biotrophic fungus Podosphaera pannosa, which causes powdery mildew, and the hemibiotrophic fungus Diplocarpon rosae, which causes black spot, we analyzed changes in the leaf transcriptome after the inoculation of detached rose leaves with each pathogen. In addition, we analyzed differences in the transcriptomic changes inflicted by both pathogens as a first step to characterize specific infection strategies. Transcriptomic changes were analyzed using next-generation sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throughput qPCR. We identified a large number of differentially regulated genes. A common set of the differentially regulated genes comprised of pathogenesis-related (PR) genes, such as of PR10 homologs, chitinases and defense-related transcription factors, such as various WRKY genes, indicating a conserved but insufficient PTI [pathogen associated molecular pattern (PAMP) triggered immunity] reaction. Surprisingly, most of the differentially regulated genes were specific to the interactions with either P. pannosa or D. rosae. Specific regulation in response to D. rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes, such as paralogs of PR1 and PR5, and other factors of the salicylic acid signaling pathway. Differently, inoculation with P. pannosa leads in addition to the general pathogen response to a downregulation of genes related to photosynthesis and cell wall modification.
Keywords
- Black spot, High-throughput qPCR, MACE analysis, Powdery mildew, PR genes, WRKY genes, Pathogen-Associated Molecular Pattern Molecules/metabolism, Gene Expression Profiling, Immunity, Gene Expression Regulation, Plant/genetics, Genes, Plant/genetics, Arabidopsis Proteins, Salicylic Acid, Rosa/genetics, Host-Pathogen Interactions/genetics, Chitinases/genetics, Plant Diseases/genetics, Plant Growth Regulators/genetics, Signal Transduction/genetics, Transcription Factors/genetics, Transcriptome/genetics, Ascomycota/pathogenicity, Plant Proteins/genetics, Flavonoids/metabolism, High-Throughput Nucleotide Sequencing, Plant Leaves/genetics
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant molecular biology, Vol. 99, No. 4-5, 01.03.2019, p. 299-316.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation
AU - Neu, Enzo
AU - Domes, Helena Sophia
AU - Menz, Ina
AU - Kaufmann, Helgard
AU - Linde, Marcus
AU - Debener, Thomas
N1 - Funding Information: Funding This work was funded by the DFG GRK 1798 “Signaling at the Plant-Soil Interface” (GRK1798/1).
PY - 2019/3/1
Y1 - 2019/3/1
N2 - KEY MESSAGE: Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification for powdery mildew. Plant pathogenic fungi successfully colonize their hosts by manipulating the host defense mechanisms, which is accompanied by major transcriptome changes in the host. To characterize compatible plant pathogen interactions at early stages of infection by the obligate biotrophic fungus Podosphaera pannosa, which causes powdery mildew, and the hemibiotrophic fungus Diplocarpon rosae, which causes black spot, we analyzed changes in the leaf transcriptome after the inoculation of detached rose leaves with each pathogen. In addition, we analyzed differences in the transcriptomic changes inflicted by both pathogens as a first step to characterize specific infection strategies. Transcriptomic changes were analyzed using next-generation sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throughput qPCR. We identified a large number of differentially regulated genes. A common set of the differentially regulated genes comprised of pathogenesis-related (PR) genes, such as of PR10 homologs, chitinases and defense-related transcription factors, such as various WRKY genes, indicating a conserved but insufficient PTI [pathogen associated molecular pattern (PAMP) triggered immunity] reaction. Surprisingly, most of the differentially regulated genes were specific to the interactions with either P. pannosa or D. rosae. Specific regulation in response to D. rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes, such as paralogs of PR1 and PR5, and other factors of the salicylic acid signaling pathway. Differently, inoculation with P. pannosa leads in addition to the general pathogen response to a downregulation of genes related to photosynthesis and cell wall modification.
AB - KEY MESSAGE: Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification for powdery mildew. Plant pathogenic fungi successfully colonize their hosts by manipulating the host defense mechanisms, which is accompanied by major transcriptome changes in the host. To characterize compatible plant pathogen interactions at early stages of infection by the obligate biotrophic fungus Podosphaera pannosa, which causes powdery mildew, and the hemibiotrophic fungus Diplocarpon rosae, which causes black spot, we analyzed changes in the leaf transcriptome after the inoculation of detached rose leaves with each pathogen. In addition, we analyzed differences in the transcriptomic changes inflicted by both pathogens as a first step to characterize specific infection strategies. Transcriptomic changes were analyzed using next-generation sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throughput qPCR. We identified a large number of differentially regulated genes. A common set of the differentially regulated genes comprised of pathogenesis-related (PR) genes, such as of PR10 homologs, chitinases and defense-related transcription factors, such as various WRKY genes, indicating a conserved but insufficient PTI [pathogen associated molecular pattern (PAMP) triggered immunity] reaction. Surprisingly, most of the differentially regulated genes were specific to the interactions with either P. pannosa or D. rosae. Specific regulation in response to D. rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes, such as paralogs of PR1 and PR5, and other factors of the salicylic acid signaling pathway. Differently, inoculation with P. pannosa leads in addition to the general pathogen response to a downregulation of genes related to photosynthesis and cell wall modification.
KW - Black spot
KW - High-throughput qPCR
KW - MACE analysis
KW - Powdery mildew
KW - PR genes
KW - WRKY genes
KW - Pathogen-Associated Molecular Pattern Molecules/metabolism
KW - Gene Expression Profiling
KW - Immunity
KW - Gene Expression Regulation, Plant/genetics
KW - Genes, Plant/genetics
KW - Arabidopsis Proteins
KW - Salicylic Acid
KW - Rosa/genetics
KW - Host-Pathogen Interactions/genetics
KW - Chitinases/genetics
KW - Plant Diseases/genetics
KW - Plant Growth Regulators/genetics
KW - Signal Transduction/genetics
KW - Transcription Factors/genetics
KW - Transcriptome/genetics
KW - Ascomycota/pathogenicity
KW - Plant Proteins/genetics
KW - Flavonoids/metabolism
KW - High-Throughput Nucleotide Sequencing
KW - Plant Leaves/genetics
UR - http://www.scopus.com/inward/record.url?scp=85061005574&partnerID=8YFLogxK
U2 - 10.1007/s11103-018-00818-2
DO - 10.1007/s11103-018-00818-2
M3 - Article
C2 - 30706286
AN - SCOPUS:85061005574
VL - 99
SP - 299
EP - 316
JO - Plant molecular biology
JF - Plant molecular biology
SN - 0167-4412
IS - 4-5
ER -