Details
| Original language | English |
|---|---|
| Article number | e3024 |
| Journal | Biotechnology progress |
| Volume | 36 |
| Issue number | 5 |
| Publication status | Published - 16 Sept 2020 |
| Externally published | Yes |
Abstract
In this study, an ion exchange resin-based downstream-processing concept for imine reductase (IRED)-catalyzed reactions was investigated. As a model reaction, 2-methylpyrroline was converted to its corresponding product (S)-2-methylpyrrolidine with >99% of conversion by the (S)-selective IRED from Paenibacillus elgii B69. Under optimized reaction conditions full conversion was achieved using a substrate concentration of 150 and 500 mmol/L of d-glucose. Seven commercially available cation- and anion-exchange resins were studied with respect to their ability to recover the product from the reaction solution. Without any pretreatment, cation-exchange resins Amberlite IR-120(H), IRN-150, Dowex Monosphere 650C, and Dowex Marathon MSC showed high recovery capacities (up to >90%). A 150-ml preparative scale reaction was performed yielding ~1 g hydrochloride salt product with >99% purity. Any further purification steps, for example, by column chromatography or recrystallization, were not required.
Keywords
- downstream-processing, enzyme, imine reductase, ion exchange resin, process development
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Biotechnology progress, Vol. 36, No. 5, e3024, 16.09.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Integration of ion exchange resin materials for a downstream‐processing approach of an imine reductase‐catalyzed reaction
AU - Meyer, Lars-Erik
AU - Brundiek, Henrike
AU - Langermann, Jan
N1 - Publisher Copyright: © 2020 The Authors. Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.
PY - 2020/9/16
Y1 - 2020/9/16
N2 - In this study, an ion exchange resin-based downstream-processing concept for imine reductase (IRED)-catalyzed reactions was investigated. As a model reaction, 2-methylpyrroline was converted to its corresponding product (S)-2-methylpyrrolidine with >99% of conversion by the (S)-selective IRED from Paenibacillus elgii B69. Under optimized reaction conditions full conversion was achieved using a substrate concentration of 150 and 500 mmol/L of d-glucose. Seven commercially available cation- and anion-exchange resins were studied with respect to their ability to recover the product from the reaction solution. Without any pretreatment, cation-exchange resins Amberlite IR-120(H), IRN-150, Dowex Monosphere 650C, and Dowex Marathon MSC showed high recovery capacities (up to >90%). A 150-ml preparative scale reaction was performed yielding ~1 g hydrochloride salt product with >99% purity. Any further purification steps, for example, by column chromatography or recrystallization, were not required.
AB - In this study, an ion exchange resin-based downstream-processing concept for imine reductase (IRED)-catalyzed reactions was investigated. As a model reaction, 2-methylpyrroline was converted to its corresponding product (S)-2-methylpyrrolidine with >99% of conversion by the (S)-selective IRED from Paenibacillus elgii B69. Under optimized reaction conditions full conversion was achieved using a substrate concentration of 150 and 500 mmol/L of d-glucose. Seven commercially available cation- and anion-exchange resins were studied with respect to their ability to recover the product from the reaction solution. Without any pretreatment, cation-exchange resins Amberlite IR-120(H), IRN-150, Dowex Monosphere 650C, and Dowex Marathon MSC showed high recovery capacities (up to >90%). A 150-ml preparative scale reaction was performed yielding ~1 g hydrochloride salt product with >99% purity. Any further purification steps, for example, by column chromatography or recrystallization, were not required.
KW - downstream-processing
KW - enzyme
KW - imine reductase
KW - ion exchange resin
KW - process development
UR - http://www.scopus.com/inward/record.url?scp=85086472291&partnerID=8YFLogxK
U2 - 10.1002/btpr.3024
DO - 10.1002/btpr.3024
M3 - Article
VL - 36
JO - Biotechnology progress
JF - Biotechnology progress
SN - 8756-7938
IS - 5
M1 - e3024
ER -