Details
| Original language | English |
|---|---|
| Pages (from-to) | 129-134 |
| Number of pages | 6 |
| Journal | CYTOTECHNOLOGY |
| Volume | 38 |
| Issue number | 1-3 |
| Publication status | Published - Jan 2002 |
Abstract
The in-situ microscope is a system developed to acquire images of mammalian cells directly inside a bioreactor (in-situ) during a fermentation process. It requires only minimal operator intervention and it is well suited for either batch or long-term perfusion fermentation runs. The system fits into a 25 mm standard port and has a retractable housing, similar to the industry standard InTrac. Therefore, it can be cleaned and serviced without interruption of the process or risking contamination. A sampling zone inside the bioreactor encloses a defined volume of culture and an image sequence is taken. The height of the sampling zone is set by the control program and can be adjusted during the cultivation to accommodate a wide range of change in cell density. The system has an infinity corrected optical train and uses a progressive scan CCD camera to acquire high quality images. Process relevant information like cell density is extracted from the images by digital image processing software, currently in development for mammalian cells (CHO, BHK). The first version of the software will be able to estimate the cell density, cell size distribution and to give information of the degree of aggregation (single and double cells, cell clusters).
Keywords
- Image analysis, In-situ microscopy, Online biomass estimation, Online bioprocess monitoring
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: CYTOTECHNOLOGY, Vol. 38, No. 1-3, 01.2002, p. 129-134.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In-situ microscopy: Online process monitoring of mammalian cell cultures
AU - Joeris, Klaus
AU - Frerichs, Jan Gerd
AU - Konstantinov, Konstantin
AU - Scheper, Thomas
PY - 2002/1
Y1 - 2002/1
N2 - The in-situ microscope is a system developed to acquire images of mammalian cells directly inside a bioreactor (in-situ) during a fermentation process. It requires only minimal operator intervention and it is well suited for either batch or long-term perfusion fermentation runs. The system fits into a 25 mm standard port and has a retractable housing, similar to the industry standard InTrac. Therefore, it can be cleaned and serviced without interruption of the process or risking contamination. A sampling zone inside the bioreactor encloses a defined volume of culture and an image sequence is taken. The height of the sampling zone is set by the control program and can be adjusted during the cultivation to accommodate a wide range of change in cell density. The system has an infinity corrected optical train and uses a progressive scan CCD camera to acquire high quality images. Process relevant information like cell density is extracted from the images by digital image processing software, currently in development for mammalian cells (CHO, BHK). The first version of the software will be able to estimate the cell density, cell size distribution and to give information of the degree of aggregation (single and double cells, cell clusters).
AB - The in-situ microscope is a system developed to acquire images of mammalian cells directly inside a bioreactor (in-situ) during a fermentation process. It requires only minimal operator intervention and it is well suited for either batch or long-term perfusion fermentation runs. The system fits into a 25 mm standard port and has a retractable housing, similar to the industry standard InTrac. Therefore, it can be cleaned and serviced without interruption of the process or risking contamination. A sampling zone inside the bioreactor encloses a defined volume of culture and an image sequence is taken. The height of the sampling zone is set by the control program and can be adjusted during the cultivation to accommodate a wide range of change in cell density. The system has an infinity corrected optical train and uses a progressive scan CCD camera to acquire high quality images. Process relevant information like cell density is extracted from the images by digital image processing software, currently in development for mammalian cells (CHO, BHK). The first version of the software will be able to estimate the cell density, cell size distribution and to give information of the degree of aggregation (single and double cells, cell clusters).
KW - Image analysis
KW - In-situ microscopy
KW - Online biomass estimation
KW - Online bioprocess monitoring
UR - http://www.scopus.com/inward/record.url?scp=0036425993&partnerID=8YFLogxK
U2 - 10.1023/A:1021170502775
DO - 10.1023/A:1021170502775
M3 - Article
AN - SCOPUS:0036425993
VL - 38
SP - 129
EP - 134
JO - CYTOTECHNOLOGY
JF - CYTOTECHNOLOGY
SN - 0920-9069
IS - 1-3
ER -