Details
| Original language | English |
|---|---|
| Title of host publication | Applied Biocatalysis |
| Subtitle of host publication | From Fundamental Science to Industrial Applications |
| Publisher | Wiley-VCH Verlag |
| Pages | 253-295 |
| Number of pages | 43 |
| ISBN (electronic) | 9783527677122 |
| ISBN (print) | 9783527336692 |
| Publication status | Published - 16 Aug 2016 |
Abstract
This chapter introduces the employed inorganic adsorbents and presents the results about enzyme purification and isolation on aluminum oxides and aluminosilicates. The most common adsorbents for biotechnological processes are especially for protein or enzyme purification of polymeric resin particles as well as cross-linked dextran gels. The chapter discusses the immobilization of phospholipases A by physisorption on precipitated silica and continues with immobilization of lipase and alcohol dehydrogenase (ADH) on precipitated silica, modified by the layer-by-layer (LbL) technology. It also addresses the development of a reaction concept using immobilized ADH in columns in a solvent-based system. It describes where a phospholipase enzyme can be used in several recycling steps in the degumming of crude oils. For the industrialization of biotechnological processes, there are still numerous challenges to be overcome associated with downstream processing. A powerful way to cope with the challenges is selective adsorption on specific solid adsorbents.
Keywords
- Alcohol dehydrogenase, Aluminum oxides, Biocatalytic transformations, Enzyme purification, Inorganic adsorbents, Layer-by-layer technology, Phospholipase enzyme
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
Cite this
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Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag, 2016. p. 253-295.
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Inorganic Adsorbents in Enzymatic Processes
AU - Sohling, Ulrich
AU - Suck, Kirstin
AU - Jonczyk, Patrick
AU - Sander, Friederike
AU - Beutel, Sascha
AU - Scheper, Thomas
AU - Thiefes, Axel
AU - Schuldt, Ute
AU - Aldenhoven, Claudia
AU - Egri, Gabriella
AU - Dähne, Lars
AU - Fiethen, Annamaria
AU - Kuhn, Hubert
AU - Wenzel, Oliver
AU - Temme, Heike
AU - Niemeyer, Bernd
AU - Bubenheim, Paul
AU - Liese, Andreas
PY - 2016/8/16
Y1 - 2016/8/16
N2 - This chapter introduces the employed inorganic adsorbents and presents the results about enzyme purification and isolation on aluminum oxides and aluminosilicates. The most common adsorbents for biotechnological processes are especially for protein or enzyme purification of polymeric resin particles as well as cross-linked dextran gels. The chapter discusses the immobilization of phospholipases A by physisorption on precipitated silica and continues with immobilization of lipase and alcohol dehydrogenase (ADH) on precipitated silica, modified by the layer-by-layer (LbL) technology. It also addresses the development of a reaction concept using immobilized ADH in columns in a solvent-based system. It describes where a phospholipase enzyme can be used in several recycling steps in the degumming of crude oils. For the industrialization of biotechnological processes, there are still numerous challenges to be overcome associated with downstream processing. A powerful way to cope with the challenges is selective adsorption on specific solid adsorbents.
AB - This chapter introduces the employed inorganic adsorbents and presents the results about enzyme purification and isolation on aluminum oxides and aluminosilicates. The most common adsorbents for biotechnological processes are especially for protein or enzyme purification of polymeric resin particles as well as cross-linked dextran gels. The chapter discusses the immobilization of phospholipases A by physisorption on precipitated silica and continues with immobilization of lipase and alcohol dehydrogenase (ADH) on precipitated silica, modified by the layer-by-layer (LbL) technology. It also addresses the development of a reaction concept using immobilized ADH in columns in a solvent-based system. It describes where a phospholipase enzyme can be used in several recycling steps in the degumming of crude oils. For the industrialization of biotechnological processes, there are still numerous challenges to be overcome associated with downstream processing. A powerful way to cope with the challenges is selective adsorption on specific solid adsorbents.
KW - Alcohol dehydrogenase
KW - Aluminum oxides
KW - Biocatalytic transformations
KW - Enzyme purification
KW - Inorganic adsorbents
KW - Layer-by-layer technology
KW - Phospholipase enzyme
UR - http://www.scopus.com/inward/record.url?scp=85017479386&partnerID=8YFLogxK
U2 - 10.1002/9783527677122.ch12
DO - 10.1002/9783527677122.ch12
M3 - Contribution to book/anthology
AN - SCOPUS:85017479386
SN - 9783527336692
SP - 253
EP - 295
BT - Applied Biocatalysis
PB - Wiley-VCH Verlag
ER -