Details
| Original language | English |
|---|---|
| Article number | 412 |
| Number of pages | 14 |
| Journal | Cells |
| Volume | 11 |
| Issue number | 3 |
| Publication status | Published - 25 Jan 2022 |
| Externally published | Yes |
Abstract
The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation.
Keywords
- 3D culture, 3R, Analytics, Cells, Microenvironment, Monitoring platform, Open flow microperfusion, Perfusion bioreactor, Sampling, Sensors
ASJC Scopus subject areas
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Cells, Vol. 11, No. 3, 412, 25.01.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures
AU - Kreß, Sebastian
AU - Schaller-Ammann, Roland
AU - Feiel, Jürgen
AU - Wegener, Joachim
AU - Priedl, Joachim
AU - Dietrich, Wolf
AU - Kasper, Cornelia
AU - Egger, Dominik
N1 - Funding Information: Funding: This research was funded by Österreichische Forschungsförderungsgesellschaft, grant number 859279.
PY - 2022/1/25
Y1 - 2022/1/25
N2 - The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation.
AB - The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation.
KW - 3D culture
KW - 3R
KW - Analytics
KW - Cells
KW - Microenvironment
KW - Monitoring platform
KW - Open flow microperfusion
KW - Perfusion bioreactor
KW - Sampling
KW - Sensors
UR - http://www.scopus.com/inward/record.url?scp=85123301631&partnerID=8YFLogxK
U2 - 10.3390/cells11030412
DO - 10.3390/cells11030412
M3 - Article
AN - SCOPUS:85123301631
VL - 11
JO - Cells
JF - Cells
SN - 2073-4409
IS - 3
M1 - 412
ER -