Details
| Original language | English |
|---|---|
| Pages (from-to) | 43-51 |
| Number of pages | 9 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 72 |
| Issue number | 1 |
| Publication status | Published - 1 Jan 2003 |
Abstract
Objective of the studies was to analyse the mode of inheritance of the regeneration ability of Cyclamen persicum via somatic embryogenesis. Three genotypes inbred to I2 differing concerning their in vitro response were self- and cross-pollinated to produce I3, F1 and F2 progenies. These progenies were tested in vitro by culturing unpollinated ovules on callus induction medium containing 9.05 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 3.94 μM 2iP (6-(γ,γ-dimethylallylamino)purine) for two passages of 8 weeks each, followed by a period of 8 weeks on hormone-free medium for differentiation of somatic embryos. Regarding the regeneration ability, uniformity was confirmed in the I3 and F1 generation indicating the homozygosity of the parental I2 plants. No differences between reciprocal cross combinations were found, so that maternal effects have been excluded. In the F2 progenies, derived from crosses between the regenerating genotypes 3051 and 3738, respectively, and the non-regenerating genotype RS8, the segregation of plants with and without regeneration of somatic embryos fit to a 15:1 ratio. Therefore, a genetic control of the regeneration ability by two dominant major genes with epistatic interaction was postulated. Regarding the regeneration rates, defined as the percentage of calluses with somatic embryos, and the regeneration intensity, as indicated by the number of somatic embryos per callus, a continuous variation between the plants with regeneration ability was noticed. These observations point to the fact that - apart from physiological and environmental influences - additional modifying genes might be involved.
Keywords
- Breeding, Cyclamen persicum, Genetic analysis, In vitro propagation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 72, No. 1, 01.01.2003, p. 43-51.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Inheritance of the ability for regeneration via somatic embryogenesis in Cyclamen persicum
AU - Pueschel, A. K.
AU - Schwenkel, H. G.
AU - Winkelmann, T.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Objective of the studies was to analyse the mode of inheritance of the regeneration ability of Cyclamen persicum via somatic embryogenesis. Three genotypes inbred to I2 differing concerning their in vitro response were self- and cross-pollinated to produce I3, F1 and F2 progenies. These progenies were tested in vitro by culturing unpollinated ovules on callus induction medium containing 9.05 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 3.94 μM 2iP (6-(γ,γ-dimethylallylamino)purine) for two passages of 8 weeks each, followed by a period of 8 weeks on hormone-free medium for differentiation of somatic embryos. Regarding the regeneration ability, uniformity was confirmed in the I3 and F1 generation indicating the homozygosity of the parental I2 plants. No differences between reciprocal cross combinations were found, so that maternal effects have been excluded. In the F2 progenies, derived from crosses between the regenerating genotypes 3051 and 3738, respectively, and the non-regenerating genotype RS8, the segregation of plants with and without regeneration of somatic embryos fit to a 15:1 ratio. Therefore, a genetic control of the regeneration ability by two dominant major genes with epistatic interaction was postulated. Regarding the regeneration rates, defined as the percentage of calluses with somatic embryos, and the regeneration intensity, as indicated by the number of somatic embryos per callus, a continuous variation between the plants with regeneration ability was noticed. These observations point to the fact that - apart from physiological and environmental influences - additional modifying genes might be involved.
AB - Objective of the studies was to analyse the mode of inheritance of the regeneration ability of Cyclamen persicum via somatic embryogenesis. Three genotypes inbred to I2 differing concerning their in vitro response were self- and cross-pollinated to produce I3, F1 and F2 progenies. These progenies were tested in vitro by culturing unpollinated ovules on callus induction medium containing 9.05 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 3.94 μM 2iP (6-(γ,γ-dimethylallylamino)purine) for two passages of 8 weeks each, followed by a period of 8 weeks on hormone-free medium for differentiation of somatic embryos. Regarding the regeneration ability, uniformity was confirmed in the I3 and F1 generation indicating the homozygosity of the parental I2 plants. No differences between reciprocal cross combinations were found, so that maternal effects have been excluded. In the F2 progenies, derived from crosses between the regenerating genotypes 3051 and 3738, respectively, and the non-regenerating genotype RS8, the segregation of plants with and without regeneration of somatic embryos fit to a 15:1 ratio. Therefore, a genetic control of the regeneration ability by two dominant major genes with epistatic interaction was postulated. Regarding the regeneration rates, defined as the percentage of calluses with somatic embryos, and the regeneration intensity, as indicated by the number of somatic embryos per callus, a continuous variation between the plants with regeneration ability was noticed. These observations point to the fact that - apart from physiological and environmental influences - additional modifying genes might be involved.
KW - Breeding
KW - Cyclamen persicum
KW - Genetic analysis
KW - In vitro propagation
UR - http://www.scopus.com/inward/record.url?scp=0037279309&partnerID=8YFLogxK
U2 - 10.1023/A:1021231806838
DO - 10.1023/A:1021231806838
M3 - Article
AN - SCOPUS:0037279309
VL - 72
SP - 43
EP - 51
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -