Details
| Original language | English |
|---|---|
| Pages (from-to) | 295-302 |
| Number of pages | 8 |
| Journal | CHEMOSPHERE |
| Volume | 117 |
| Issue number | 1 |
| Early online date | 13 Aug 2014 |
| Publication status | Published - Dec 2014 |
Abstract
The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL-1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.
Keywords
- Antibiotics, Bacterial community, Nitrifying biofilms, Resistance genes, Tetracycline removal
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Engineering
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- General Chemistry
- Environmental Science(all)
- Pollution
- Environmental Science(all)
- Health, Toxicology and Mutagenesis
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In: CHEMOSPHERE, Vol. 117, No. 1, 12.2014, p. 295-302.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms
AU - Matos, Maria
AU - Pereira, Maria A.
AU - Parpot, Pier
AU - Brito, António G.
AU - Nogueira, Regina
N1 - Funding Information: The work described in this paper was financially supported by FCT – Foundation for Science and Technology (Portugal) – by the PhD student grant SFRH/BD/44596/2008 .
PY - 2014/12
Y1 - 2014/12
N2 - The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL-1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.
AB - The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50μgL-1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.
KW - Antibiotics
KW - Bacterial community
KW - Nitrifying biofilms
KW - Resistance genes
KW - Tetracycline removal
UR - http://www.scopus.com/inward/record.url?scp=84922240302&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2014.06.094
DO - 10.1016/j.chemosphere.2014.06.094
M3 - Article
C2 - 25127228
AN - SCOPUS:84922240302
VL - 117
SP - 295
EP - 302
JO - CHEMOSPHERE
JF - CHEMOSPHERE
SN - 0045-6535
IS - 1
ER -