Details
| Original language | English |
|---|---|
| Pages (from-to) | 1048-1060 |
| Number of pages | 13 |
| Journal | Journal of the American Society for Mass Spectrometry |
| Volume | 33 |
| Issue number | 6 |
| Early online date | 20 May 2022 |
| Publication status | Published - 1 Jun 2022 |
Abstract
High Kinetic Energy Ion Mobility Spectrometers (HiKE-IMS) chemically ionize gaseous samples via reactant ions and separate the generated ions by their motion in a neutral gas under the influence of an electric field. Operation at reduced pressures of 10-40 mbar allows for reaching high reduced electric field strengths (E/N) of up to 120 Td. At these high E/N, the generated ions gain the namesake high kinetic energies, leading to a decrease in cluster size of the reactant ions by increasing the reaction rate of collision-induced cluster dissociation of hydrates. In positive ion polarity and in purified air, H 3O +(H 2O) n, NO +(H 2O) n, and O 2 +•(H 2O) pare the most abundant reactant ions. In this work, we investigate the effect of varying sample gas humidity on product ion formation for several model substances. Results show that increasing the sample gas humidity at high E/N of 120 Td shifts product ion formation from a charge transfer dominated reaction system to a proton transfer dominated reaction system. For HiKE-IMS operated at high E/N, the reduction in cluster size of reactant ions allows ionization of analytes with low proton affinity even at high relative humidity in the sample gas of RH = 75% at 303.15 K and 1013.25 hPa. In contrast to conventional IMS, where increasing the sample gas humidity inhibits ionization for various analytes, increasing sample gas humidity in HiKE-IMS operated at 120 Td is actually beneficial for ionization yield of most analytes investigated in this work as it increases the number of H 3O +(H 2O)n.
Keywords
- high kinetic energy ion mobility spectrometry, humidity, ion mobility spectrometry, ionization, moisture
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Structural Biology
- Chemistry(all)
- Spectroscopy
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In: Journal of the American Society for Mass Spectrometry, Vol. 33, No. 6, 01.06.2022, p. 1048-1060.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Influence of Sample Gas Humidity on Product Ion Formation in High Kinetic Energy Ion Mobility Spectrometry (HiKE-IMS)
AU - Schaefer, Christoph
AU - Schlottmann, Florian
AU - Kirk, Ansgar T.
AU - Zimmermann, Stefan
N1 - Funding Information: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 318063177 and 390583968.
PY - 2022/6/1
Y1 - 2022/6/1
N2 - High Kinetic Energy Ion Mobility Spectrometers (HiKE-IMS) chemically ionize gaseous samples via reactant ions and separate the generated ions by their motion in a neutral gas under the influence of an electric field. Operation at reduced pressures of 10-40 mbar allows for reaching high reduced electric field strengths (E/N) of up to 120 Td. At these high E/N, the generated ions gain the namesake high kinetic energies, leading to a decrease in cluster size of the reactant ions by increasing the reaction rate of collision-induced cluster dissociation of hydrates. In positive ion polarity and in purified air, H 3O +(H 2O) n, NO +(H 2O) n, and O 2 +•(H 2O) pare the most abundant reactant ions. In this work, we investigate the effect of varying sample gas humidity on product ion formation for several model substances. Results show that increasing the sample gas humidity at high E/N of 120 Td shifts product ion formation from a charge transfer dominated reaction system to a proton transfer dominated reaction system. For HiKE-IMS operated at high E/N, the reduction in cluster size of reactant ions allows ionization of analytes with low proton affinity even at high relative humidity in the sample gas of RH = 75% at 303.15 K and 1013.25 hPa. In contrast to conventional IMS, where increasing the sample gas humidity inhibits ionization for various analytes, increasing sample gas humidity in HiKE-IMS operated at 120 Td is actually beneficial for ionization yield of most analytes investigated in this work as it increases the number of H 3O +(H 2O)n.
AB - High Kinetic Energy Ion Mobility Spectrometers (HiKE-IMS) chemically ionize gaseous samples via reactant ions and separate the generated ions by their motion in a neutral gas under the influence of an electric field. Operation at reduced pressures of 10-40 mbar allows for reaching high reduced electric field strengths (E/N) of up to 120 Td. At these high E/N, the generated ions gain the namesake high kinetic energies, leading to a decrease in cluster size of the reactant ions by increasing the reaction rate of collision-induced cluster dissociation of hydrates. In positive ion polarity and in purified air, H 3O +(H 2O) n, NO +(H 2O) n, and O 2 +•(H 2O) pare the most abundant reactant ions. In this work, we investigate the effect of varying sample gas humidity on product ion formation for several model substances. Results show that increasing the sample gas humidity at high E/N of 120 Td shifts product ion formation from a charge transfer dominated reaction system to a proton transfer dominated reaction system. For HiKE-IMS operated at high E/N, the reduction in cluster size of reactant ions allows ionization of analytes with low proton affinity even at high relative humidity in the sample gas of RH = 75% at 303.15 K and 1013.25 hPa. In contrast to conventional IMS, where increasing the sample gas humidity inhibits ionization for various analytes, increasing sample gas humidity in HiKE-IMS operated at 120 Td is actually beneficial for ionization yield of most analytes investigated in this work as it increases the number of H 3O +(H 2O)n.
KW - high kinetic energy ion mobility spectrometry
KW - humidity
KW - ion mobility spectrometry
KW - ionization
KW - moisture
UR - http://www.scopus.com/inward/record.url?scp=85131216835&partnerID=8YFLogxK
U2 - 10.1021/jasms.2c00099
DO - 10.1021/jasms.2c00099
M3 - Article
VL - 33
SP - 1048
EP - 1060
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
SN - 1044-0305
IS - 6
ER -