Industrial Application of Membrane Chromatography for the Purification of Enzymes

Research output: Chapter in book/report/conference proceedingContribution to book/anthologyResearchpeer review

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  • Sartorius AG
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Details

Original languageEnglish
Title of host publicationApplied Biocatalysis
Subtitle of host publicationFrom Fundamental Science to Industrial Applications
PublisherWiley-VCH Verlag
Pages297-316
Number of pages20
ISBN (electronic)9783527677122
ISBN (print)9783527336692
Publication statusPublished - 16 Aug 2016

Abstract

Very often, Chromatographic methods are used especially for the separation and purification of biomolecules as they typically allow high selectivity and process robustness. Many downstream processes consist of at least two or more chromatographic steps to produce the pure biomolecule. The basic membrane adsorber consists of functionalized macroporous membranes with ligands coupled on the inner specific surface area. This chapter shows all variants of membrane adsorbers that used for the evaluation of the basic binding conditions of the model enzymes. It evaluates the potential of membrane adsorbers for the isolation and purification of industrial relevant enzymes. The chapter presents the three exemplary case studies that demonstrate the suitability of the membrane adsorber technology for enzyme purification. Various membrane adsorbers were tested and a successful purification strategy could be developed for each of the employed model enzymes penicillin G amidase (PGA), cellulase Cel5a, and lipase aGTL.

Keywords

    Biocatalysis, Cellulase, Downstream processing, Enzyme purification, Lipase, Membrane chromatography, Penicillin G amidase

ASJC Scopus subject areas

Cite this

Industrial Application of Membrane Chromatography for the Purification of Enzymes. / Beutel, Sascha; Villain, Louis; Scheper, Thomas.
Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag, 2016. p. 297-316.

Research output: Chapter in book/report/conference proceedingContribution to book/anthologyResearchpeer review

Beutel, S, Villain, L & Scheper, T 2016, Industrial Application of Membrane Chromatography for the Purification of Enzymes. in Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag, pp. 297-316. https://doi.org/10.1002/9783527677122.ch13
Beutel, S., Villain, L., & Scheper, T. (2016). Industrial Application of Membrane Chromatography for the Purification of Enzymes. In Applied Biocatalysis: From Fundamental Science to Industrial Applications (pp. 297-316). Wiley-VCH Verlag. https://doi.org/10.1002/9783527677122.ch13
Beutel S, Villain L, Scheper T. Industrial Application of Membrane Chromatography for the Purification of Enzymes. In Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag. 2016. p. 297-316 doi: 10.1002/9783527677122.ch13
Beutel, Sascha ; Villain, Louis ; Scheper, Thomas. / Industrial Application of Membrane Chromatography for the Purification of Enzymes. Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag, 2016. pp. 297-316
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