Details
| Original language | English |
|---|---|
| Title of host publication | Applied Biocatalysis |
| Subtitle of host publication | From Fundamental Science to Industrial Applications |
| Publisher | Wiley-VCH Verlag |
| Pages | 297-316 |
| Number of pages | 20 |
| ISBN (electronic) | 9783527677122 |
| ISBN (print) | 9783527336692 |
| Publication status | Published - 16 Aug 2016 |
Abstract
Very often, Chromatographic methods are used especially for the separation and purification of biomolecules as they typically allow high selectivity and process robustness. Many downstream processes consist of at least two or more chromatographic steps to produce the pure biomolecule. The basic membrane adsorber consists of functionalized macroporous membranes with ligands coupled on the inner specific surface area. This chapter shows all variants of membrane adsorbers that used for the evaluation of the basic binding conditions of the model enzymes. It evaluates the potential of membrane adsorbers for the isolation and purification of industrial relevant enzymes. The chapter presents the three exemplary case studies that demonstrate the suitability of the membrane adsorber technology for enzyme purification. Various membrane adsorbers were tested and a successful purification strategy could be developed for each of the employed model enzymes penicillin G amidase (PGA), cellulase Cel5a, and lipase aGTL.
Keywords
- Biocatalysis, Cellulase, Downstream processing, Enzyme purification, Lipase, Membrane chromatography, Penicillin G amidase
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
Cite this
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Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag, 2016. p. 297-316.
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Industrial Application of Membrane Chromatography for the Purification of Enzymes
AU - Beutel, Sascha
AU - Villain, Louis
AU - Scheper, Thomas
PY - 2016/8/16
Y1 - 2016/8/16
N2 - Very often, Chromatographic methods are used especially for the separation and purification of biomolecules as they typically allow high selectivity and process robustness. Many downstream processes consist of at least two or more chromatographic steps to produce the pure biomolecule. The basic membrane adsorber consists of functionalized macroporous membranes with ligands coupled on the inner specific surface area. This chapter shows all variants of membrane adsorbers that used for the evaluation of the basic binding conditions of the model enzymes. It evaluates the potential of membrane adsorbers for the isolation and purification of industrial relevant enzymes. The chapter presents the three exemplary case studies that demonstrate the suitability of the membrane adsorber technology for enzyme purification. Various membrane adsorbers were tested and a successful purification strategy could be developed for each of the employed model enzymes penicillin G amidase (PGA), cellulase Cel5a, and lipase aGTL.
AB - Very often, Chromatographic methods are used especially for the separation and purification of biomolecules as they typically allow high selectivity and process robustness. Many downstream processes consist of at least two or more chromatographic steps to produce the pure biomolecule. The basic membrane adsorber consists of functionalized macroporous membranes with ligands coupled on the inner specific surface area. This chapter shows all variants of membrane adsorbers that used for the evaluation of the basic binding conditions of the model enzymes. It evaluates the potential of membrane adsorbers for the isolation and purification of industrial relevant enzymes. The chapter presents the three exemplary case studies that demonstrate the suitability of the membrane adsorber technology for enzyme purification. Various membrane adsorbers were tested and a successful purification strategy could be developed for each of the employed model enzymes penicillin G amidase (PGA), cellulase Cel5a, and lipase aGTL.
KW - Biocatalysis
KW - Cellulase
KW - Downstream processing
KW - Enzyme purification
KW - Lipase
KW - Membrane chromatography
KW - Penicillin G amidase
UR - http://www.scopus.com/inward/record.url?scp=85017442315&partnerID=8YFLogxK
U2 - 10.1002/9783527677122.ch13
DO - 10.1002/9783527677122.ch13
M3 - Contribution to book/anthology
AN - SCOPUS:85017442315
SN - 9783527336692
SP - 297
EP - 316
BT - Applied Biocatalysis
PB - Wiley-VCH Verlag
ER -