Details
| Original language | English |
|---|---|
| Article number | 160178 |
| Journal | Science of the Total Environment |
| Volume | 858 |
| Early online date | 13 Nov 2022 |
| Publication status | Published - 1 Feb 2023 |
Abstract
Sewage wastewater pollutes water and poses a public health issue but it could also prove useful in certain research domains. Sewage is a complex niche relevant for research concerning ‘one-health’, human health, pollution and antibiotic resistance. Indian gut microbiome is also understudied due to sampling constraints and sewage could be used to explore it. Ostensibly, Indian sewage needs to be studied and here, we performed a cross-sectional pan-India sewage sampling to generate the first comprehensive Indian sewage microbiome. Indian sewage showed predominance of Burkholderiaceae, Rhodocyclaceae, Veillonellaceae, Prevotellaceae, etc. and has high representation of gut microbes. The identified gut microbes have overrepresentation of Veillonellaceae, Rikenellaceae, Streptococcaceae, and Bacillaceae. Imputed metagenomics of sewage microbiome indicated dominance of transport, motility, peptidases, amino acid metabolism, and antibiotic resistance genes. Microbiome-disease associations drawn using simple decision tree and random forest analysis identified specific microbes as potential predictors of diabetes and obesity in a city. Altogether, we generated the first Indian sewage microbiome and our non-invasive, high-throughput workflow could be emulated for future research, wastewater-based epidemiology and designing policies concerning public health.
Keywords
- Indian gut microbiome, Indian sewage microbiome, Next-generation sequencing, One-health and microbiome, Sewage treatment plant, Wastewater-based epidemiology
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Engineering
- Environmental Science(all)
- Environmental Chemistry
- Environmental Science(all)
- Waste Management and Disposal
- Environmental Science(all)
- Pollution
Sustainable Development Goals
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In: Science of the Total Environment, Vol. 858, 160178, 01.02.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Indian sewage microbiome has unique community characteristics and potential for population-level disease predictions
AU - Singh, Kumar Siddharth
AU - Paul, Dhiraj
AU - Gupta, Abhishek
AU - Dhotre, Dhiraj
AU - Klawonn, Frank
AU - Shouche, Yogesh
N1 - Funding Information: KSS received National Postdoctoral Fellowship (Grant ID: PDF/2016/003317 ) from Science and Engineering Research Board , Department of Science & Technology, India .
PY - 2023/2/1
Y1 - 2023/2/1
N2 - Sewage wastewater pollutes water and poses a public health issue but it could also prove useful in certain research domains. Sewage is a complex niche relevant for research concerning ‘one-health’, human health, pollution and antibiotic resistance. Indian gut microbiome is also understudied due to sampling constraints and sewage could be used to explore it. Ostensibly, Indian sewage needs to be studied and here, we performed a cross-sectional pan-India sewage sampling to generate the first comprehensive Indian sewage microbiome. Indian sewage showed predominance of Burkholderiaceae, Rhodocyclaceae, Veillonellaceae, Prevotellaceae, etc. and has high representation of gut microbes. The identified gut microbes have overrepresentation of Veillonellaceae, Rikenellaceae, Streptococcaceae, and Bacillaceae. Imputed metagenomics of sewage microbiome indicated dominance of transport, motility, peptidases, amino acid metabolism, and antibiotic resistance genes. Microbiome-disease associations drawn using simple decision tree and random forest analysis identified specific microbes as potential predictors of diabetes and obesity in a city. Altogether, we generated the first Indian sewage microbiome and our non-invasive, high-throughput workflow could be emulated for future research, wastewater-based epidemiology and designing policies concerning public health.
AB - Sewage wastewater pollutes water and poses a public health issue but it could also prove useful in certain research domains. Sewage is a complex niche relevant for research concerning ‘one-health’, human health, pollution and antibiotic resistance. Indian gut microbiome is also understudied due to sampling constraints and sewage could be used to explore it. Ostensibly, Indian sewage needs to be studied and here, we performed a cross-sectional pan-India sewage sampling to generate the first comprehensive Indian sewage microbiome. Indian sewage showed predominance of Burkholderiaceae, Rhodocyclaceae, Veillonellaceae, Prevotellaceae, etc. and has high representation of gut microbes. The identified gut microbes have overrepresentation of Veillonellaceae, Rikenellaceae, Streptococcaceae, and Bacillaceae. Imputed metagenomics of sewage microbiome indicated dominance of transport, motility, peptidases, amino acid metabolism, and antibiotic resistance genes. Microbiome-disease associations drawn using simple decision tree and random forest analysis identified specific microbes as potential predictors of diabetes and obesity in a city. Altogether, we generated the first Indian sewage microbiome and our non-invasive, high-throughput workflow could be emulated for future research, wastewater-based epidemiology and designing policies concerning public health.
KW - Indian gut microbiome
KW - Indian sewage microbiome
KW - Next-generation sequencing
KW - One-health and microbiome
KW - Sewage treatment plant
KW - Wastewater-based epidemiology
UR - http://www.scopus.com/inward/record.url?scp=85142183161&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2022.160178
DO - 10.1016/j.scitotenv.2022.160178
M3 - Article
C2 - 36379333
AN - SCOPUS:85142183161
VL - 858
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 160178
ER -