Indian sewage microbiome has unique community characteristics and potential for population-level disease predictions

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Kumar Siddharth Singh
  • Dhiraj Paul
  • Abhishek Gupta
  • Dhiraj Dhotre
  • Frank Klawonn
  • Yogesh Shouche

Research Organisations

External Research Organisations

  • National Centre for Cell Science
  • Helmholtz Centre for Infection Research (HZI)
  • Azim Premji University
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Details

Original languageEnglish
Article number160178
JournalScience of the Total Environment
Volume858
Early online date13 Nov 2022
Publication statusPublished - 1 Feb 2023

Abstract

Sewage wastewater pollutes water and poses a public health issue but it could also prove useful in certain research domains. Sewage is a complex niche relevant for research concerning ‘one-health’, human health, pollution and antibiotic resistance. Indian gut microbiome is also understudied due to sampling constraints and sewage could be used to explore it. Ostensibly, Indian sewage needs to be studied and here, we performed a cross-sectional pan-India sewage sampling to generate the first comprehensive Indian sewage microbiome. Indian sewage showed predominance of Burkholderiaceae, Rhodocyclaceae, Veillonellaceae, Prevotellaceae, etc. and has high representation of gut microbes. The identified gut microbes have overrepresentation of Veillonellaceae, Rikenellaceae, Streptococcaceae, and Bacillaceae. Imputed metagenomics of sewage microbiome indicated dominance of transport, motility, peptidases, amino acid metabolism, and antibiotic resistance genes. Microbiome-disease associations drawn using simple decision tree and random forest analysis identified specific microbes as potential predictors of diabetes and obesity in a city. Altogether, we generated the first Indian sewage microbiome and our non-invasive, high-throughput workflow could be emulated for future research, wastewater-based epidemiology and designing policies concerning public health.

Keywords

    Indian gut microbiome, Indian sewage microbiome, Next-generation sequencing, One-health and microbiome, Sewage treatment plant, Wastewater-based epidemiology

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Indian sewage microbiome has unique community characteristics and potential for population-level disease predictions. / Singh, Kumar Siddharth; Paul, Dhiraj; Gupta, Abhishek et al.
In: Science of the Total Environment, Vol. 858, 160178, 01.02.2023.

Research output: Contribution to journalArticleResearchpeer review

Singh KS, Paul D, Gupta A, Dhotre D, Klawonn F, Shouche Y. Indian sewage microbiome has unique community characteristics and potential for population-level disease predictions. Science of the Total Environment. 2023 Feb 1;858:160178. Epub 2022 Nov 13. doi: 10.1016/j.scitotenv.2022.160178
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