Details
| Original language | English |
|---|---|
| Pages (from-to) | 1190-1202 |
| Number of pages | 13 |
| Journal | Journal of Tissue Engineering and Regenerative Medicine |
| Volume | 13 |
| Issue number | 7 |
| Early online date | 26 Apr 2019 |
| Publication status | Published - 15 Jul 2019 |
Abstract
In orthopaedic medicine, connective tissues are often affected by traumatic or degenerative injuries, and surgical intervention is required. Rotator cuff tears are a common cause of shoulder pain and disability among adults. The development of graft materials for bridging the gap between tendon and bone after chronic rotator cuff tears is essentially required. The limiting factor for the clinical success of a tissue engineering construct is a fast and complete vascularization of the construct. Otherwise, immigrating cells are not able to survive for a longer period of time, resulting in the failure of the graft material. The femur chamber allows the observation of microhaemodynamic parameters inside implants located in close vicinity to the femur in repeated measurements in vivo. We compared a porous polymer patch (a commercially available porous polyurethane-based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fibre mats and chitosan (CS)-graft-PCL modified electrospun PCL (CS-g-PCL) fibre mats in vivo. By means of intravital fluorescence microscopy, microhaemodynamic parameters were analysed repetitively over 20 days at intervals of 3 to 4 days. CS-g-PCL modified fibre mats showed a significantly increased vascularization at Day 10 compared with Day 6 and at Day 14 compared with the porous polymer patch and the unmodified PCL fibre mats at the same day. These results could be verified by histology. In conclusion, a clear improvement in terms of vascularization and biocompatibility is achieved by graft-copolymer modification compared with the unmodified material.
Keywords
- angiogenesis, biocompatibility, electrospinning, intravital microscopy, microhaemodynamics, PCL fibre mats
ASJC Scopus subject areas
- Medicine(all)
- Medicine (miscellaneous)
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
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In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 13, No. 7, 15.07.2019, p. 1190-1202.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In vivo analysis of vascularization and biocompatibility of electrospun polycaprolactone fibre mats in the rat femur chamber
AU - Gniesmer, Sarah
AU - Brehm, Ralph
AU - Hoffmann, Andrea
AU - de Cassan, Dominik
AU - Menzel, Henning
AU - Hoheisel, Anna Lena
AU - Glasmacher, Birgit
AU - Willbold, Elmar
AU - Reifenrath, Janin
AU - Wellmann, Mathias
AU - Ludwig, Nils
AU - Tavassol, Frank
AU - Zimmerer, Ruediger
AU - Gellrich, Nils Claudius
AU - Kampmann, Andreas
N1 - Funding Information: This research project has been supported by the DFG FOR 2180 “Gradierte Implantate für Sehnen‐Knochen‐Verbindungen” (KA 4236/1‐1). We acknowledge the excellent technical assistance of Stefanie Rausch.
PY - 2019/7/15
Y1 - 2019/7/15
N2 - In orthopaedic medicine, connective tissues are often affected by traumatic or degenerative injuries, and surgical intervention is required. Rotator cuff tears are a common cause of shoulder pain and disability among adults. The development of graft materials for bridging the gap between tendon and bone after chronic rotator cuff tears is essentially required. The limiting factor for the clinical success of a tissue engineering construct is a fast and complete vascularization of the construct. Otherwise, immigrating cells are not able to survive for a longer period of time, resulting in the failure of the graft material. The femur chamber allows the observation of microhaemodynamic parameters inside implants located in close vicinity to the femur in repeated measurements in vivo. We compared a porous polymer patch (a commercially available porous polyurethane-based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fibre mats and chitosan (CS)-graft-PCL modified electrospun PCL (CS-g-PCL) fibre mats in vivo. By means of intravital fluorescence microscopy, microhaemodynamic parameters were analysed repetitively over 20 days at intervals of 3 to 4 days. CS-g-PCL modified fibre mats showed a significantly increased vascularization at Day 10 compared with Day 6 and at Day 14 compared with the porous polymer patch and the unmodified PCL fibre mats at the same day. These results could be verified by histology. In conclusion, a clear improvement in terms of vascularization and biocompatibility is achieved by graft-copolymer modification compared with the unmodified material.
AB - In orthopaedic medicine, connective tissues are often affected by traumatic or degenerative injuries, and surgical intervention is required. Rotator cuff tears are a common cause of shoulder pain and disability among adults. The development of graft materials for bridging the gap between tendon and bone after chronic rotator cuff tears is essentially required. The limiting factor for the clinical success of a tissue engineering construct is a fast and complete vascularization of the construct. Otherwise, immigrating cells are not able to survive for a longer period of time, resulting in the failure of the graft material. The femur chamber allows the observation of microhaemodynamic parameters inside implants located in close vicinity to the femur in repeated measurements in vivo. We compared a porous polymer patch (a commercially available porous polyurethane-based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fibre mats and chitosan (CS)-graft-PCL modified electrospun PCL (CS-g-PCL) fibre mats in vivo. By means of intravital fluorescence microscopy, microhaemodynamic parameters were analysed repetitively over 20 days at intervals of 3 to 4 days. CS-g-PCL modified fibre mats showed a significantly increased vascularization at Day 10 compared with Day 6 and at Day 14 compared with the porous polymer patch and the unmodified PCL fibre mats at the same day. These results could be verified by histology. In conclusion, a clear improvement in terms of vascularization and biocompatibility is achieved by graft-copolymer modification compared with the unmodified material.
KW - angiogenesis
KW - biocompatibility
KW - electrospinning
KW - intravital microscopy
KW - microhaemodynamics
KW - PCL fibre mats
UR - http://www.scopus.com/inward/record.url?scp=85066481026&partnerID=8YFLogxK
U2 - 10.1002/term.2868
DO - 10.1002/term.2868
M3 - Article
C2 - 31025510
AN - SCOPUS:85066481026
VL - 13
SP - 1190
EP - 1202
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 7
ER -