Details
| Original language | English |
|---|---|
| Article number | 298 |
| Journal | Journal of nanoparticle research |
| Volume | 17 |
| Issue number | 7 |
| Publication status | Published - 11 Jul 2015 |
Abstract
The use of nanoparticles is becoming increasingly common in industry and everyday objects. Thus, extensive risk management concerning the potential health risk of nanoparticles is important. Currently, in vitro nanoparticle testing is mainly performed under static culture conditions without any shear stress. However, shear stress is an important biomechanical parameter. Therefore, in this study, a defined physiological flow to different mammalian cell lines such as A549 cells and NIH-3T3 cells has been applied. The effects of zinc oxide and titanium dioxide nanoparticles (TiO2-NP), respectively, were investigated under both static and dynamic conditions. Cell viability, cell morphology, and adhesion were proven and compared to the static cell culture. Flow exposure had an impact on the cellular morphology of the cells. NIH-3T3 cells were elongated in the direction of flow and A549 cells exhibited vesicles inside the cells. Zinc oxide nanoparticles reduced the cell viability in the static and in the dynamic culture; however, the dynamic cultures were more sensitive. In the static culture and in the dynamic culture, TiO2-NP did not affect cell viability. In conclusion, dynamic culture conditions are important for further in vitro investigations and provide more relevant results than static culture conditions.
Keywords
- Mammalian cells, Physiological flow, Shear stress, Titanium dioxide nanoparticles, Zinc oxide nanoparticles
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Chemistry(all)
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Mathematics(all)
- Modelling and Simulation
- Materials Science(all)
- Physics and Astronomy(all)
- Condensed Matter Physics
Sustainable Development Goals
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In: Journal of nanoparticle research, Vol. 17, No. 7, 298, 11.07.2015.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In vitro toxicological nanoparticle studies under flow exposure
AU - Sambale, Franziska
AU - Stahl, Frank
AU - Bahnemann, Detlef
AU - Scheper, Thomas
N1 - Funding information: This work was supported by the European Regional Development Fund (EFRE Project “Nanokomp”, Grant No.: 60421066). Detlef Bahnemann kindly acknowledges support by the project “Establishment of the Laboratory of Photoactive Nanocomposites Materials” (No. 14.750.31.0016) supported by a Grant from the Government of the Russian Federation.
PY - 2015/7/11
Y1 - 2015/7/11
N2 - The use of nanoparticles is becoming increasingly common in industry and everyday objects. Thus, extensive risk management concerning the potential health risk of nanoparticles is important. Currently, in vitro nanoparticle testing is mainly performed under static culture conditions without any shear stress. However, shear stress is an important biomechanical parameter. Therefore, in this study, a defined physiological flow to different mammalian cell lines such as A549 cells and NIH-3T3 cells has been applied. The effects of zinc oxide and titanium dioxide nanoparticles (TiO2-NP), respectively, were investigated under both static and dynamic conditions. Cell viability, cell morphology, and adhesion were proven and compared to the static cell culture. Flow exposure had an impact on the cellular morphology of the cells. NIH-3T3 cells were elongated in the direction of flow and A549 cells exhibited vesicles inside the cells. Zinc oxide nanoparticles reduced the cell viability in the static and in the dynamic culture; however, the dynamic cultures were more sensitive. In the static culture and in the dynamic culture, TiO2-NP did not affect cell viability. In conclusion, dynamic culture conditions are important for further in vitro investigations and provide more relevant results than static culture conditions.
AB - The use of nanoparticles is becoming increasingly common in industry and everyday objects. Thus, extensive risk management concerning the potential health risk of nanoparticles is important. Currently, in vitro nanoparticle testing is mainly performed under static culture conditions without any shear stress. However, shear stress is an important biomechanical parameter. Therefore, in this study, a defined physiological flow to different mammalian cell lines such as A549 cells and NIH-3T3 cells has been applied. The effects of zinc oxide and titanium dioxide nanoparticles (TiO2-NP), respectively, were investigated under both static and dynamic conditions. Cell viability, cell morphology, and adhesion were proven and compared to the static cell culture. Flow exposure had an impact on the cellular morphology of the cells. NIH-3T3 cells were elongated in the direction of flow and A549 cells exhibited vesicles inside the cells. Zinc oxide nanoparticles reduced the cell viability in the static and in the dynamic culture; however, the dynamic cultures were more sensitive. In the static culture and in the dynamic culture, TiO2-NP did not affect cell viability. In conclusion, dynamic culture conditions are important for further in vitro investigations and provide more relevant results than static culture conditions.
KW - Mammalian cells
KW - Physiological flow
KW - Shear stress
KW - Titanium dioxide nanoparticles
KW - Zinc oxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84937440163&partnerID=8YFLogxK
U2 - 10.1007/s11051-015-3106-2
DO - 10.1007/s11051-015-3106-2
M3 - Article
AN - SCOPUS:84937440163
VL - 17
JO - Journal of nanoparticle research
JF - Journal of nanoparticle research
SN - 1388-0764
IS - 7
M1 - 298
ER -