Details
| Original language | English |
|---|---|
| Pages (from-to) | 14922-14931 |
| Number of pages | 10 |
| Journal | RSC Advances |
| Volume | 11 |
| Issue number | 25 |
| Publication status | Published - 21 Apr 2021 |
Abstract
In vitro assays of enzymes involved in the biosynthesis of maleidrides from polyketides in fungi were performed. The results show that the enzymes are closely related to primary metabolism enzymes of the citric acid cycle in terms of stereochemical preferences, but with an expanded substrate selectivity. A key citrate synthase can react both saturated and unsaturated acyl CoA substrates to give solely anti substituted citrates. This undergoes anti-dehydration to afford an unsaturated precursor which is cyclised in vitro by ketosteroid-isomerase-like enzymes to give byssochlamic acid. This journal is
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Chemical Engineering(all)
- General Chemical Engineering
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In: RSC Advances, Vol. 11, No. 25, 21.04.2021, p. 14922-14931.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In vitro studies of maleidride-forming enzymes
AU - Yin, Sen
AU - Friedrich, Steffen
AU - Hrupins, Vjaceslavs
AU - Cox, Russell J.
N1 - Funding Information: We thank Ben Schleibaum for assistance with the synthetic chemistry. The China Scholarship Council is thanked for funding (SY, CSC201606210136). DFG is thanked for analytical instrumentation (INST 187/686-1) and funding for VH (CO 1328/ 3-1). Prof. Jianqiang Kong is thanked for sending us yeast plasmids and strains. The publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2021/4/21
Y1 - 2021/4/21
N2 - In vitro assays of enzymes involved in the biosynthesis of maleidrides from polyketides in fungi were performed. The results show that the enzymes are closely related to primary metabolism enzymes of the citric acid cycle in terms of stereochemical preferences, but with an expanded substrate selectivity. A key citrate synthase can react both saturated and unsaturated acyl CoA substrates to give solely anti substituted citrates. This undergoes anti-dehydration to afford an unsaturated precursor which is cyclised in vitro by ketosteroid-isomerase-like enzymes to give byssochlamic acid. This journal is
AB - In vitro assays of enzymes involved in the biosynthesis of maleidrides from polyketides in fungi were performed. The results show that the enzymes are closely related to primary metabolism enzymes of the citric acid cycle in terms of stereochemical preferences, but with an expanded substrate selectivity. A key citrate synthase can react both saturated and unsaturated acyl CoA substrates to give solely anti substituted citrates. This undergoes anti-dehydration to afford an unsaturated precursor which is cyclised in vitro by ketosteroid-isomerase-like enzymes to give byssochlamic acid. This journal is
UR - http://www.scopus.com/inward/record.url?scp=85105802947&partnerID=8YFLogxK
U2 - 10.1039/d1ra02118d
DO - 10.1039/d1ra02118d
M3 - Article
VL - 11
SP - 14922
EP - 14931
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 25
ER -