Details
| Original language | English |
|---|---|
| Article number | 190 |
| Journal | Environmental Monitoring and Assessment |
| Volume | 189 |
| Issue number | 4 |
| Publication status | Published - Apr 2017 |
Abstract
Thousands of gallons of industrial chemicals, crude 4-methylcyclohexanemethanol (MCHM) and propylene glycol phenyl ether (PPh), leaked from industrial tanks into the Elk River in Charleston, West Virginia, USA, on January 9, 2014. A considerable number of people were reported to exhibit symptoms of chemical exposure and an estimated 300,000 residents were advised not to use or drink tap water. At the time of the spill, the existing toxicological data of the chemicals were limited for a full evaluation of the health risks, resulting in concern among those in the impacted regions. In this preliminary study, we assessed cell viability and plasma membrane degradation following a 24-h exposure to varying concentrations (0–1000 μM) of the two compounds, alone and in combination. Evaluation of different cell lines, HEK-293 (kidney), HepG2 (liver), H9c2 (heart), and GT1-7 (brain), provided insight regarding altered cellular responses in varying organ systems. Single exposure to MCHM or PPh did not affect cell viability, except at doses much higher than the estimated exposure levels. Certain co-exposures significantly reduced metabolic activity and increased plasma membrane degradation in GT1-7, HepG2, and H9c2 cells. These findings highlight the importance of examining co-exposures to fully understand the potential toxic effects.
Keywords
- 4-methylcyclohexanemethanol, Chemical spill, Co-exposures, Cytotoxicity, Propylene glycol phenyl ether
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Science(all)
- Pollution
- Environmental Science(all)
- Management, Monitoring, Policy and Law
Sustainable Development Goals
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In: Environmental Monitoring and Assessment, Vol. 189, No. 4, 190, 04.2017.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In vitro cytotoxicity assessment of a West Virginia chemical spill mixture involving 4-methylcyclohexanemethanol and propylene glycol phenyl ether
AU - Han, Alice A.
AU - Fabyanic, Emily B.
AU - Miller, Julie V.
AU - Prediger, Maren S.
AU - Prince, Nicole
AU - Mouch, Julia A.
AU - Boyd, Jonathan
N1 - Publisher Copyright: © 2017, Springer International Publishing Switzerland.
PY - 2017/4
Y1 - 2017/4
N2 - Thousands of gallons of industrial chemicals, crude 4-methylcyclohexanemethanol (MCHM) and propylene glycol phenyl ether (PPh), leaked from industrial tanks into the Elk River in Charleston, West Virginia, USA, on January 9, 2014. A considerable number of people were reported to exhibit symptoms of chemical exposure and an estimated 300,000 residents were advised not to use or drink tap water. At the time of the spill, the existing toxicological data of the chemicals were limited for a full evaluation of the health risks, resulting in concern among those in the impacted regions. In this preliminary study, we assessed cell viability and plasma membrane degradation following a 24-h exposure to varying concentrations (0–1000 μM) of the two compounds, alone and in combination. Evaluation of different cell lines, HEK-293 (kidney), HepG2 (liver), H9c2 (heart), and GT1-7 (brain), provided insight regarding altered cellular responses in varying organ systems. Single exposure to MCHM or PPh did not affect cell viability, except at doses much higher than the estimated exposure levels. Certain co-exposures significantly reduced metabolic activity and increased plasma membrane degradation in GT1-7, HepG2, and H9c2 cells. These findings highlight the importance of examining co-exposures to fully understand the potential toxic effects.
AB - Thousands of gallons of industrial chemicals, crude 4-methylcyclohexanemethanol (MCHM) and propylene glycol phenyl ether (PPh), leaked from industrial tanks into the Elk River in Charleston, West Virginia, USA, on January 9, 2014. A considerable number of people were reported to exhibit symptoms of chemical exposure and an estimated 300,000 residents were advised not to use or drink tap water. At the time of the spill, the existing toxicological data of the chemicals were limited for a full evaluation of the health risks, resulting in concern among those in the impacted regions. In this preliminary study, we assessed cell viability and plasma membrane degradation following a 24-h exposure to varying concentrations (0–1000 μM) of the two compounds, alone and in combination. Evaluation of different cell lines, HEK-293 (kidney), HepG2 (liver), H9c2 (heart), and GT1-7 (brain), provided insight regarding altered cellular responses in varying organ systems. Single exposure to MCHM or PPh did not affect cell viability, except at doses much higher than the estimated exposure levels. Certain co-exposures significantly reduced metabolic activity and increased plasma membrane degradation in GT1-7, HepG2, and H9c2 cells. These findings highlight the importance of examining co-exposures to fully understand the potential toxic effects.
KW - 4-methylcyclohexanemethanol
KW - Chemical spill
KW - Co-exposures
KW - Cytotoxicity
KW - Propylene glycol phenyl ether
UR - http://www.scopus.com/inward/record.url?scp=85016596296&partnerID=8YFLogxK
U2 - 10.1007/s10661-017-5895-5
DO - 10.1007/s10661-017-5895-5
M3 - Article
VL - 189
JO - Environmental Monitoring and Assessment
JF - Environmental Monitoring and Assessment
SN - 0167-6369
IS - 4
M1 - 190
ER -