Details
| Original language | English |
|---|---|
| Article number | 96 |
| Journal | Journal of nanobiotechnology |
| Volume | 16 |
| Publication status | Published - 27 Nov 2018 |
Abstract
Background: In orthopedic surgery, implant-associated infections are still a major problem. For the improvement of the selective therapy in the infection area, magnetic nanoparticles as drug carriers are promising when used in combination with magnetizable implants and an externally applied magnetic field. These implants principally increase the strength of the magnetic field resulting in an enhanced accumulation of the drug loaded particles in the target area and therewith a reduction of the needed amount and the risk of undesirable side effects. In the present study magnetic nanoporous silica core-shell nanoparticles, modified with fluorophores (fluorescein isothiocyanate/FITC or rhodamine B isothiocyanate/RITC) and poly(ethylene glycol) (PEG), were used in combination with metallic plates of different magnetic properties and with a magnetic field. In vitro and in vivo experiments were performed to investigate particle accumulation and retention and their biocompatibility. Results: Spherical magnetic silica core-shell nanoparticles with reproducible superparamagnetic behavior and high porosity were synthesized. Based on in vitro proliferation and viability tests the modification with organic fluorophores and PEG led to highly biocompatible fluorescent particles, and good dispersibility. In a circular tube system martensitic steel 1.4112 showed superior accumulation and retention of the magnetic particles in comparison to ferritic steel 1.4521 and a Ti90Al6V4 control. In vivo tests in a mouse model where the nanoparticles were injected subcutaneously showed the good biocompatibility of the magnetic silica nanoparticles and their accumulation on the surface of a metallic plate, which had been implanted before, and in the surrounding tissue. Conclusion: With their superparamagnetic properties and their high porosity, multifunctional magnetic nanoporous silica nanoparticles are ideal candidates as drug carriers. In combination with their good biocompatibility in vitro, they have ideal properties for an implant directed magnetic drug targeting. Missing adverse clinical and histological effects proved the good biocompatibility in vivo. Accumulation and retention of the nanoparticles could be influenced by the magnetic properties of the implanted plates; a remanent martensitic steel plate significantly improved both values in vitro. Therefore, the use of magnetizable implant materials in combination with the magnetic nanoparticles has promising potential for the selective treatment of implant-associated infections.
Keywords
- Biocompatibility, Core-shell nanoparticles, Drug targeting, Ferritic steel, Martensitic steel, Mouse model, Nanoporous silica, PEGylation, Superparamagnetic FeO, NIH 3T3 Cells, Silicon Dioxide/chemistry, Biocompatible Materials/chemistry, Humans, Magnetite Nanoparticles/chemistry, Prostheses and Implants, Nanopores, Hep G2 Cells, Drug Carriers/chemistry, Animals, Female, Mice, Mice, Inbred BALB C, Magnetic Fields
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Engineering(all)
- Biomedical Engineering
- Medicine(all)
- Medicine (miscellaneous)
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
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In: Journal of nanobiotechnology, Vol. 16, 96, 27.11.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In vitro and in vivo accumulation of magnetic nanoporous silica nanoparticles on implant materials with different magnetic properties
AU - Janßen, Hilke Catherina
AU - Warwas, Dawid Peter
AU - Dahlhaus, David
AU - Meißner, Jessica
AU - Taptimthong, Piriya
AU - Kietzmann, Manfred
AU - Behrens, Peter
AU - Reifenrath, Janin
AU - Angrisani, Nina
N1 - Funding information: This work was supported by the project “Implant-Directed Magnetic Drug Targeting: Antibiotic therapy of peri-implant infections”, Project Number: 280642759, which was funded by Deutsche Forschungsgemeinschaft (DE): RE 3456/2-1.
PY - 2018/11/27
Y1 - 2018/11/27
N2 - Background: In orthopedic surgery, implant-associated infections are still a major problem. For the improvement of the selective therapy in the infection area, magnetic nanoparticles as drug carriers are promising when used in combination with magnetizable implants and an externally applied magnetic field. These implants principally increase the strength of the magnetic field resulting in an enhanced accumulation of the drug loaded particles in the target area and therewith a reduction of the needed amount and the risk of undesirable side effects. In the present study magnetic nanoporous silica core-shell nanoparticles, modified with fluorophores (fluorescein isothiocyanate/FITC or rhodamine B isothiocyanate/RITC) and poly(ethylene glycol) (PEG), were used in combination with metallic plates of different magnetic properties and with a magnetic field. In vitro and in vivo experiments were performed to investigate particle accumulation and retention and their biocompatibility. Results: Spherical magnetic silica core-shell nanoparticles with reproducible superparamagnetic behavior and high porosity were synthesized. Based on in vitro proliferation and viability tests the modification with organic fluorophores and PEG led to highly biocompatible fluorescent particles, and good dispersibility. In a circular tube system martensitic steel 1.4112 showed superior accumulation and retention of the magnetic particles in comparison to ferritic steel 1.4521 and a Ti90Al6V4 control. In vivo tests in a mouse model where the nanoparticles were injected subcutaneously showed the good biocompatibility of the magnetic silica nanoparticles and their accumulation on the surface of a metallic plate, which had been implanted before, and in the surrounding tissue. Conclusion: With their superparamagnetic properties and their high porosity, multifunctional magnetic nanoporous silica nanoparticles are ideal candidates as drug carriers. In combination with their good biocompatibility in vitro, they have ideal properties for an implant directed magnetic drug targeting. Missing adverse clinical and histological effects proved the good biocompatibility in vivo. Accumulation and retention of the nanoparticles could be influenced by the magnetic properties of the implanted plates; a remanent martensitic steel plate significantly improved both values in vitro. Therefore, the use of magnetizable implant materials in combination with the magnetic nanoparticles has promising potential for the selective treatment of implant-associated infections.
AB - Background: In orthopedic surgery, implant-associated infections are still a major problem. For the improvement of the selective therapy in the infection area, magnetic nanoparticles as drug carriers are promising when used in combination with magnetizable implants and an externally applied magnetic field. These implants principally increase the strength of the magnetic field resulting in an enhanced accumulation of the drug loaded particles in the target area and therewith a reduction of the needed amount and the risk of undesirable side effects. In the present study magnetic nanoporous silica core-shell nanoparticles, modified with fluorophores (fluorescein isothiocyanate/FITC or rhodamine B isothiocyanate/RITC) and poly(ethylene glycol) (PEG), were used in combination with metallic plates of different magnetic properties and with a magnetic field. In vitro and in vivo experiments were performed to investigate particle accumulation and retention and their biocompatibility. Results: Spherical magnetic silica core-shell nanoparticles with reproducible superparamagnetic behavior and high porosity were synthesized. Based on in vitro proliferation and viability tests the modification with organic fluorophores and PEG led to highly biocompatible fluorescent particles, and good dispersibility. In a circular tube system martensitic steel 1.4112 showed superior accumulation and retention of the magnetic particles in comparison to ferritic steel 1.4521 and a Ti90Al6V4 control. In vivo tests in a mouse model where the nanoparticles were injected subcutaneously showed the good biocompatibility of the magnetic silica nanoparticles and their accumulation on the surface of a metallic plate, which had been implanted before, and in the surrounding tissue. Conclusion: With their superparamagnetic properties and their high porosity, multifunctional magnetic nanoporous silica nanoparticles are ideal candidates as drug carriers. In combination with their good biocompatibility in vitro, they have ideal properties for an implant directed magnetic drug targeting. Missing adverse clinical and histological effects proved the good biocompatibility in vivo. Accumulation and retention of the nanoparticles could be influenced by the magnetic properties of the implanted plates; a remanent martensitic steel plate significantly improved both values in vitro. Therefore, the use of magnetizable implant materials in combination with the magnetic nanoparticles has promising potential for the selective treatment of implant-associated infections.
KW - Biocompatibility
KW - Core-shell nanoparticles
KW - Drug targeting
KW - Ferritic steel
KW - Martensitic steel
KW - Mouse model
KW - Nanoporous silica
KW - PEGylation
KW - Superparamagnetic FeO
KW - NIH 3T3 Cells
KW - Silicon Dioxide/chemistry
KW - Biocompatible Materials/chemistry
KW - Humans
KW - Magnetite Nanoparticles/chemistry
KW - Prostheses and Implants
KW - Nanopores
KW - Hep G2 Cells
KW - Drug Carriers/chemistry
KW - Animals
KW - Female
KW - Mice
KW - Mice, Inbred BALB C
KW - Magnetic Fields
UR - http://www.scopus.com/inward/record.url?scp=85057217312&partnerID=8YFLogxK
U2 - 10.1186/s12951-018-0422-6
DO - 10.1186/s12951-018-0422-6
M3 - Article
C2 - 30482189
AN - SCOPUS:85057217312
VL - 16
JO - Journal of nanobiotechnology
JF - Journal of nanobiotechnology
SN - 1477-3155
M1 - 96
ER -