Details
| Original language | English |
|---|---|
| Pages (from-to) | 106-116 |
| Number of pages | 11 |
| Journal | Biotechnology and Bioengineering |
| Volume | 47 |
| Issue number | 1 |
| Publication status | Published - 5 Jul 1995 |
| Externally published | Yes |
Abstract
A new technique is presented which allows the use of a front‐end sensor head for in situ and on‐line characterization of cell concentration and cell size during fermentation. An epifluorescence microscope is mounted in a port of a bioreactor viewing directly into the agitated broth. Still images from cells are generated using pulsed illumination. They are directly visualized on a monitor and used for automatic image analysis. The cell concentration and morphological information are determined by counting and evaluating the cell images with respect to their depth from focus characteristic. An in situ microscope was successfully tested during yeast fermentations and yielded results which correlated well with results from a hemocytometer. © 1995 John Wiley & Sons, Inc.
Keywords
- cell concentration, depth from focus, image analysis, in situ microscopy, on‐line biomass determination
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Biotechnology and Bioengineering, Vol. 47, No. 1, 05.07.1995, p. 106-116.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In Situ Microscopy for On-Line Characterization of Cell-Populations in Bioreactors, Including Cell-Concentration Measurements by Depth from Focus
AU - Suhr, H.
AU - Wehnert, G.
AU - Schneider, K.
AU - Bittner, C.
AU - Scholz, T.
AU - Geissler, P.
AU - Jähne, B.
AU - Scheper, T.
PY - 1995/7/5
Y1 - 1995/7/5
N2 - A new technique is presented which allows the use of a front‐end sensor head for in situ and on‐line characterization of cell concentration and cell size during fermentation. An epifluorescence microscope is mounted in a port of a bioreactor viewing directly into the agitated broth. Still images from cells are generated using pulsed illumination. They are directly visualized on a monitor and used for automatic image analysis. The cell concentration and morphological information are determined by counting and evaluating the cell images with respect to their depth from focus characteristic. An in situ microscope was successfully tested during yeast fermentations and yielded results which correlated well with results from a hemocytometer. © 1995 John Wiley & Sons, Inc.
AB - A new technique is presented which allows the use of a front‐end sensor head for in situ and on‐line characterization of cell concentration and cell size during fermentation. An epifluorescence microscope is mounted in a port of a bioreactor viewing directly into the agitated broth. Still images from cells are generated using pulsed illumination. They are directly visualized on a monitor and used for automatic image analysis. The cell concentration and morphological information are determined by counting and evaluating the cell images with respect to their depth from focus characteristic. An in situ microscope was successfully tested during yeast fermentations and yielded results which correlated well with results from a hemocytometer. © 1995 John Wiley & Sons, Inc.
KW - cell concentration
KW - depth from focus
KW - image analysis
KW - in situ microscopy
KW - on‐line biomass determination
UR - http://www.scopus.com/inward/record.url?scp=0029636675&partnerID=8YFLogxK
U2 - 10.1002/bit.260470113
DO - 10.1002/bit.260470113
M3 - Article
AN - SCOPUS:0029636675
VL - 47
SP - 106
EP - 116
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 1
ER -