Details
| Original language | English |
|---|---|
| Pages (from-to) | 8121-8126 |
| Number of pages | 6 |
| Journal | Analytical chemistry |
| Volume | 85 |
| Issue number | 17 |
| Publication status | Published - 29 Jul 2013 |
Abstract
A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
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In: Analytical chemistry, Vol. 85, No. 17, 29.07.2013, p. 8121-8126.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In Situ Microscopy for In-line Monitoring of the Enzymatic Hydrolysis of Cellulose
AU - Opitz, Britta
AU - Prediger, Andreas
AU - Lüder, Christian
AU - Eckstein, Marrit
AU - Hilterhaus, Lutz
AU - Lindner, Patrick
AU - Beutel, Sascha
AU - Scheper, Thomas
AU - Liese, Andreas
PY - 2013/7/29
Y1 - 2013/7/29
N2 - A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography.
AB - A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography.
UR - http://www.scopus.com/inward/record.url?scp=84883485152&partnerID=8YFLogxK
U2 - 10.1021/ac4008495
DO - 10.1021/ac4008495
M3 - Article
C2 - 23889679
AN - SCOPUS:84883485152
VL - 85
SP - 8121
EP - 8126
JO - Analytical chemistry
JF - Analytical chemistry
SN - 0003-2700
IS - 17
ER -