Details
| Original language | English |
|---|---|
| Pages (from-to) | 53-60 |
| Number of pages | 8 |
| Journal | Journal of biotechnology |
| Volume | 296 |
| Early online date | 18 Mar 2019 |
| Publication status | Published - 20 Apr 2019 |
Abstract
Microbial contamination in mammalian cell cultures causing rejected batches is costly and highly unwanted. Most methods for detecting a contamination are time-consuming and require extensive off-line sampling. To circumvent these efforts and provide a more convenient alternative, we used an online in situ microscope to estimate the cell diameter of the cellular species in the culture to distinguish mammalian cells from microbial cells depending on their size. A warning system was set up to alert the operator if microbial cells were present in the culture. Hybridoma cells were cultured and infected with either Candida utilis or Pichia stipitis as contaminant. The warning system could successfully detect the introduced contamination and alert the operator. The results suggest that in situ microscopy could be used as an efficient online tool for early detection of contaminations in cell cultures.
Keywords
- Candida, Hybridoma cells, Image analysis, Infection, Mammalian cell culture, Pichia
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of biotechnology, Vol. 296, 20.04.2019, p. 53-60.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - In situ microscopy as online tool for detecting microbial contaminations in cell culture
AU - Gustavsson, R.
AU - Mandenius, C. F.
AU - Löfgren, S.
AU - Scheper, Thomas
AU - Lindner, Patrick
N1 - Funding information: We thank Patrik Ekenberg for software communication development and Jenny Joensuu for valuable technical contributions. The authors also acknowledge funding from the Technical Faculty of Linköping University and from German Federal Ministry of Education and Research (BMBF-project: Knowledge-based process intelligence, BMBF-Bioeconomy)
PY - 2019/4/20
Y1 - 2019/4/20
N2 - Microbial contamination in mammalian cell cultures causing rejected batches is costly and highly unwanted. Most methods for detecting a contamination are time-consuming and require extensive off-line sampling. To circumvent these efforts and provide a more convenient alternative, we used an online in situ microscope to estimate the cell diameter of the cellular species in the culture to distinguish mammalian cells from microbial cells depending on their size. A warning system was set up to alert the operator if microbial cells were present in the culture. Hybridoma cells were cultured and infected with either Candida utilis or Pichia stipitis as contaminant. The warning system could successfully detect the introduced contamination and alert the operator. The results suggest that in situ microscopy could be used as an efficient online tool for early detection of contaminations in cell cultures.
AB - Microbial contamination in mammalian cell cultures causing rejected batches is costly and highly unwanted. Most methods for detecting a contamination are time-consuming and require extensive off-line sampling. To circumvent these efforts and provide a more convenient alternative, we used an online in situ microscope to estimate the cell diameter of the cellular species in the culture to distinguish mammalian cells from microbial cells depending on their size. A warning system was set up to alert the operator if microbial cells were present in the culture. Hybridoma cells were cultured and infected with either Candida utilis or Pichia stipitis as contaminant. The warning system could successfully detect the introduced contamination and alert the operator. The results suggest that in situ microscopy could be used as an efficient online tool for early detection of contaminations in cell cultures.
KW - Candida
KW - Hybridoma cells
KW - Image analysis
KW - Infection
KW - Mammalian cell culture
KW - Pichia
UR - http://www.scopus.com/inward/record.url?scp=85063193683&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2019.03.011
DO - 10.1016/j.jbiotec.2019.03.011
M3 - Article
C2 - 30898686
AN - SCOPUS:85063193683
VL - 296
SP - 53
EP - 60
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
ER -