Details
| Original language | English |
|---|---|
| Pages (from-to) | 1058-1066 |
| Number of pages | 9 |
| Journal | RSC Sustainability |
| Volume | 2 |
| Issue number | 4 |
| Early online date | 8 Mar 2024 |
| Publication status | Published - 2024 |
Abstract
Natural Deep Eutectic Solvents (NADESs) are composed of naturally occurring compounds such as organic acids, amines and sugars; they are biodegradable and have low toxicities. NADESs have gained attention not only as environmentally friendly solvents, but also for their enzyme stabilization effects at high temperatures or osmotic stress. Six betaine-based NADESs were tested regarding their impact on enzymatic activity and thermal stability of a dye-decolourizing peroxidase from the basidiomycete Pleurotus sapidus (PsaPOX). Betaine : sucrose : water (2 : 1 : 10) and betaine : trehalose : water (4 : 1 : 14) had the best impact on the thermostability. Coloured silk was discoloured more efficiently by PsaPOX after addition of either NADES, which indicates a potential as stain-removal agents. The yield of the enzymatic transformation of trans-anethole to the aroma compound p-anisaldehyde increased by a factor of three with both tested NADESs. Overall, it was shown that betaine-based NADESs serve as sustainable media for reactions using PsaPOX and increase temperature stability as well as enzymatic activity. They might thus improve the industrial usability of PsaPOX.
ASJC Scopus subject areas
- Chemistry(all)
- Chemistry (miscellaneous)
- Chemistry(all)
- Analytical Chemistry
- Chemistry(all)
- Electrochemistry
- Chemistry(all)
- Organic Chemistry
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In: RSC Sustainability, Vol. 2, No. 4, 2024, p. 1058-1066.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Improving the stability and activity of a dye-decolourizing peroxidase using NADESs
AU - Garbe, Maria
AU - Lutz, Linnea Ute
AU - Lehmann, Leander Tom
AU - Strotmann, Theresa
AU - Berger, Ralf G.
AU - Ersoy, Franziska
PY - 2024
Y1 - 2024
N2 - Natural Deep Eutectic Solvents (NADESs) are composed of naturally occurring compounds such as organic acids, amines and sugars; they are biodegradable and have low toxicities. NADESs have gained attention not only as environmentally friendly solvents, but also for their enzyme stabilization effects at high temperatures or osmotic stress. Six betaine-based NADESs were tested regarding their impact on enzymatic activity and thermal stability of a dye-decolourizing peroxidase from the basidiomycete Pleurotus sapidus (PsaPOX). Betaine : sucrose : water (2 : 1 : 10) and betaine : trehalose : water (4 : 1 : 14) had the best impact on the thermostability. Coloured silk was discoloured more efficiently by PsaPOX after addition of either NADES, which indicates a potential as stain-removal agents. The yield of the enzymatic transformation of trans-anethole to the aroma compound p-anisaldehyde increased by a factor of three with both tested NADESs. Overall, it was shown that betaine-based NADESs serve as sustainable media for reactions using PsaPOX and increase temperature stability as well as enzymatic activity. They might thus improve the industrial usability of PsaPOX.
AB - Natural Deep Eutectic Solvents (NADESs) are composed of naturally occurring compounds such as organic acids, amines and sugars; they are biodegradable and have low toxicities. NADESs have gained attention not only as environmentally friendly solvents, but also for their enzyme stabilization effects at high temperatures or osmotic stress. Six betaine-based NADESs were tested regarding their impact on enzymatic activity and thermal stability of a dye-decolourizing peroxidase from the basidiomycete Pleurotus sapidus (PsaPOX). Betaine : sucrose : water (2 : 1 : 10) and betaine : trehalose : water (4 : 1 : 14) had the best impact on the thermostability. Coloured silk was discoloured more efficiently by PsaPOX after addition of either NADES, which indicates a potential as stain-removal agents. The yield of the enzymatic transformation of trans-anethole to the aroma compound p-anisaldehyde increased by a factor of three with both tested NADESs. Overall, it was shown that betaine-based NADESs serve as sustainable media for reactions using PsaPOX and increase temperature stability as well as enzymatic activity. They might thus improve the industrial usability of PsaPOX.
UR - http://www.scopus.com/inward/record.url?scp=85187491039&partnerID=8YFLogxK
U2 - 10.1039/d3su00359k
DO - 10.1039/d3su00359k
M3 - Article
AN - SCOPUS:85187491039
VL - 2
SP - 1058
EP - 1066
JO - RSC Sustainability
JF - RSC Sustainability
SN - 2753-8125
IS - 4
ER -