Details
| Original language | English |
|---|---|
| Pages (from-to) | 52-56 |
| Number of pages | 5 |
| Journal | Scientia horticulturae |
| Volume | 220 |
| Early online date | 1 Apr 2017 |
| Publication status | Published - 16 Jun 2017 |
Abstract
The influence of a four-day dark treatment and a 48 h IBA pulse on in vitro root formation of Prunus avium microshoots was examined. Both treatments led to significantly higher rooting percentages in the majority of the six genotypes. While the IBA pulse led to a disproportional growth of roots compared to shoots, the plantlets under the dark treatment during root induction were healthy and were not visually distinct from the control plants. Further data collected during the commercial production of 17 genotypes verified the improved root induction using the dark treatment and identified the gelling agent Gelcarin (a carrageenan) as an additional factor with positive effects on rooting. In addition, the importance of in vitro rooting for the successful transfer to greenhouse conditions was confirmed. Rooted plantlets (>3 roots) had a significantly higher survival percentage and showed improved growth during acclimatization compared to unrooted microshoots.
Keywords
- Acclimatization, Adventitious rooting, Cherry, Commercial in vitro production, IBA, Micropropagation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Scientia horticulturae, Vol. 220, 16.06.2017, p. 52-56.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Improved in vitro rooting of Prunus avium microshoots using a dark treatment and an auxin pulse
AU - Quambusch, Mona
AU - Gruß, Silke
AU - Pscherer, Toni
AU - Winkelmann, Traud
AU - Bartsch, Melanie
N1 - Publisher Copyright: © 2017 Elsevier B.V. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/6/16
Y1 - 2017/6/16
N2 - The influence of a four-day dark treatment and a 48 h IBA pulse on in vitro root formation of Prunus avium microshoots was examined. Both treatments led to significantly higher rooting percentages in the majority of the six genotypes. While the IBA pulse led to a disproportional growth of roots compared to shoots, the plantlets under the dark treatment during root induction were healthy and were not visually distinct from the control plants. Further data collected during the commercial production of 17 genotypes verified the improved root induction using the dark treatment and identified the gelling agent Gelcarin (a carrageenan) as an additional factor with positive effects on rooting. In addition, the importance of in vitro rooting for the successful transfer to greenhouse conditions was confirmed. Rooted plantlets (>3 roots) had a significantly higher survival percentage and showed improved growth during acclimatization compared to unrooted microshoots.
AB - The influence of a four-day dark treatment and a 48 h IBA pulse on in vitro root formation of Prunus avium microshoots was examined. Both treatments led to significantly higher rooting percentages in the majority of the six genotypes. While the IBA pulse led to a disproportional growth of roots compared to shoots, the plantlets under the dark treatment during root induction were healthy and were not visually distinct from the control plants. Further data collected during the commercial production of 17 genotypes verified the improved root induction using the dark treatment and identified the gelling agent Gelcarin (a carrageenan) as an additional factor with positive effects on rooting. In addition, the importance of in vitro rooting for the successful transfer to greenhouse conditions was confirmed. Rooted plantlets (>3 roots) had a significantly higher survival percentage and showed improved growth during acclimatization compared to unrooted microshoots.
KW - Acclimatization
KW - Adventitious rooting
KW - Cherry
KW - Commercial in vitro production
KW - IBA
KW - Micropropagation
UR - http://www.scopus.com/inward/record.url?scp=85016556420&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2017.03.020
DO - 10.1016/j.scienta.2017.03.020
M3 - Article
AN - SCOPUS:85016556420
VL - 220
SP - 52
EP - 56
JO - Scientia horticulturae
JF - Scientia horticulturae
SN - 0304-4238
ER -