Details
| Original language | English |
|---|---|
| Pages (from-to) | 2555-2566 |
| Number of pages | 12 |
| Journal | Theoretical and applied genetics |
| Volume | 131 |
| Issue number | 12 |
| Early online date | 29 Aug 2018 |
| Publication status | Published - Dec 2018 |
Abstract
KEY MESSAGE: We achieved improved mapping resolution of the major wart resistance locus Xla-TNL containing also Sen1 in a dihaploid population using SNP data and developed additional markers with diagnostic value in tetraploid varieties. We analyzed a segregating monoparental dihaploid potato population comprising 215 genotypes derived from a tetraploid variety that is highly resistant to Synchytrium endobioticum pathotypes 18 and 6. The clear bimodal segregation for both pathotypes indicated that a major dominant resistance factor in a simplex allele configuration was present in the tetraploid donor genotype. Compared to that in previous analyses of the same tetraploid donor in conventional crosses with susceptible tetraploid genotypes, a segregation pattern with a reduced genetic complexity of resistance in dihaploids was observed here. Using the 12.8 k SolCAP SNP array, we mapped a resistance locus to the Xla-TNL region containing also Sen1 on potato chromosome 11. The improved mapping resolution provided by the monoparental dihaploids allowed for the localization of the genes responsible for the resistance to both pathotypes in an interval spanning less than 800 kbp on the reference genome. Furthermore, we identified eight molecular markers segregating without recombination to pathotype 18 and pathotype 6 resistance. Also, two developed markers display improved diagnostic properties in an independent panel of tetraploid varieties. Overall, our data provide the highest resolution mapping of wart resistance genes at the Xla-TNL locus thus far.
Keywords
- Alleles, Chromosome Mapping, Chytridiomycota/pathogenicity, Disease Resistance/genetics, Genes, Plant, Genetic Markers, Genotype, Microsatellite Repeats, Phenotype, Plant Diseases/genetics, Plant Tumors/genetics, Polymorphism, Single Nucleotide, Polymorphism, Single-Stranded Conformational, Solanum tuberosum/genetics, Tetraploidy
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Theoretical and applied genetics, Vol. 131, No. 12, 12.2018, p. 2555-2566.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Improved genetic resolution for linkage mapping of resistance to potato wart in monoparental dihaploids with potential diagnostic value in tetraploid potato varieties
AU - Bartkiewicz, Annette
AU - Chilla, Friederike
AU - Terefe-Ayana, Diro
AU - Lübeck, Jens
AU - Strahwald, Josef
AU - Tacke, Eckhard
AU - Hofferbert, Hans Reinhard
AU - Flath, Kerstin
AU - Linde, Marcus
AU - Debener, Thomas
N1 - Funding Information: Acknowledgements The study was supported by the Federal Ministry of Food and Agriculture via the Fachagentur Nachwachsende Rohstoffe e.V. (FNR). We thank Dr. Frank Schaarschmidt from the Institute for Biostatistics at the Leibniz University Hannover for helping with the statistical analysis of the SNP marker data.
PY - 2018/12
Y1 - 2018/12
N2 - KEY MESSAGE: We achieved improved mapping resolution of the major wart resistance locus Xla-TNL containing also Sen1 in a dihaploid population using SNP data and developed additional markers with diagnostic value in tetraploid varieties. We analyzed a segregating monoparental dihaploid potato population comprising 215 genotypes derived from a tetraploid variety that is highly resistant to Synchytrium endobioticum pathotypes 18 and 6. The clear bimodal segregation for both pathotypes indicated that a major dominant resistance factor in a simplex allele configuration was present in the tetraploid donor genotype. Compared to that in previous analyses of the same tetraploid donor in conventional crosses with susceptible tetraploid genotypes, a segregation pattern with a reduced genetic complexity of resistance in dihaploids was observed here. Using the 12.8 k SolCAP SNP array, we mapped a resistance locus to the Xla-TNL region containing also Sen1 on potato chromosome 11. The improved mapping resolution provided by the monoparental dihaploids allowed for the localization of the genes responsible for the resistance to both pathotypes in an interval spanning less than 800 kbp on the reference genome. Furthermore, we identified eight molecular markers segregating without recombination to pathotype 18 and pathotype 6 resistance. Also, two developed markers display improved diagnostic properties in an independent panel of tetraploid varieties. Overall, our data provide the highest resolution mapping of wart resistance genes at the Xla-TNL locus thus far.
AB - KEY MESSAGE: We achieved improved mapping resolution of the major wart resistance locus Xla-TNL containing also Sen1 in a dihaploid population using SNP data and developed additional markers with diagnostic value in tetraploid varieties. We analyzed a segregating monoparental dihaploid potato population comprising 215 genotypes derived from a tetraploid variety that is highly resistant to Synchytrium endobioticum pathotypes 18 and 6. The clear bimodal segregation for both pathotypes indicated that a major dominant resistance factor in a simplex allele configuration was present in the tetraploid donor genotype. Compared to that in previous analyses of the same tetraploid donor in conventional crosses with susceptible tetraploid genotypes, a segregation pattern with a reduced genetic complexity of resistance in dihaploids was observed here. Using the 12.8 k SolCAP SNP array, we mapped a resistance locus to the Xla-TNL region containing also Sen1 on potato chromosome 11. The improved mapping resolution provided by the monoparental dihaploids allowed for the localization of the genes responsible for the resistance to both pathotypes in an interval spanning less than 800 kbp on the reference genome. Furthermore, we identified eight molecular markers segregating without recombination to pathotype 18 and pathotype 6 resistance. Also, two developed markers display improved diagnostic properties in an independent panel of tetraploid varieties. Overall, our data provide the highest resolution mapping of wart resistance genes at the Xla-TNL locus thus far.
KW - Alleles
KW - Chromosome Mapping
KW - Chytridiomycota/pathogenicity
KW - Disease Resistance/genetics
KW - Genes, Plant
KW - Genetic Markers
KW - Genotype
KW - Microsatellite Repeats
KW - Phenotype
KW - Plant Diseases/genetics
KW - Plant Tumors/genetics
KW - Polymorphism, Single Nucleotide
KW - Polymorphism, Single-Stranded Conformational
KW - Solanum tuberosum/genetics
KW - Tetraploidy
UR - http://www.scopus.com/inward/record.url?scp=85053258874&partnerID=8YFLogxK
U2 - 10.1007/s00122-018-3172-9
DO - 10.1007/s00122-018-3172-9
M3 - Article
C2 - 30159644
AN - SCOPUS:85053258874
VL - 131
SP - 2555
EP - 2566
JO - Theoretical and applied genetics
JF - Theoretical and applied genetics
SN - 0040-5752
IS - 12
ER -