Details
| Original language | English |
|---|---|
| Title of host publication | IIR Workshop on Cold Applications in Life Sciences - Proceedings |
| Pages | 84-90 |
| Number of pages | 7 |
| ISBN (electronic) | 9782362150197 |
| Publication status | Published - 2016 |
| Event | 2016 IIR Workshop on Cold Applications in Life Sciences - Dresden, Germany Duration: 8 Sept 2016 → 9 Sept 2016 |
Publication series
| Name | Refrigeration Science and Technology |
|---|---|
| ISSN (Print) | 0151-1637 |
Abstract
Effective long-term storage of rare and clinically relevant cells depends on the cell type and thus requires optimization of the main process parameters involved in cryopreservation. Among these parameters, the cooling and thawing rates, as well as the temperature of nucleation can be adjusted by a specific cryopreservation method. In this work, we reveal the optimal conditions for cryopreservation of human fibroblasts (HF), human pulmonary microvascular endothelial cells (HPMECs), amnion (aMSCs), and bone marrow stem cells (bMSCs) using an electro-freezing method and applying 2.5 %, 5 % or 10 % (v/v) dimethyl sulfoxide (Me2SO) as a cryoprotective agent. The optimal nucleation temperature for freezing of HF was -10°C for a cooling rate of 1 K·min-1 and -7.5°C for 5 K·min-1 using 5 % Me2SO. Application of a cooling rate of 5 K·min-1 and induction of the ice formation at -12°C resulted in 90 % of viable HPMECs. The aMSCs and bMSCs reflected the highest viability of 75 % after freezing using a two-step freezing protocol utilizing a cooling rate of 7.5 K·min-1 down to -30°C and 3 K·min-1 down to -80°C. The highest cell viability was observed while inducing the ice formation at -10°C for both aMSCs and bMSCs.
ASJC Scopus subject areas
- Engineering(all)
- Control and Systems Engineering
- Engineering(all)
- Electrical and Electronic Engineering
- Engineering(all)
- Mechanical Engineering
- Physics and Astronomy(all)
- Condensed Matter Physics
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IIR Workshop on Cold Applications in Life Sciences - Proceedings. 2016. p. 84-90 (Refrigeration Science and Technology).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Importance of controlled ice formation for efficient cell biobanking
AU - Lauterboeck, L.
AU - Gryshkov, O.
AU - Hofmann, N.
AU - Glasmacher, B.
N1 - Funding Information: This work was partially supported by the German Research Foundation through the cluster of excellence REBIRTH (from REgenerative BIology to Reconstructive THerapy) (DFG, EXC 62/1). The authors want to acknowledge PD Dr. Müller and Prof. Kirkpatrick for a kind donation of the cells.
PY - 2016
Y1 - 2016
N2 - Effective long-term storage of rare and clinically relevant cells depends on the cell type and thus requires optimization of the main process parameters involved in cryopreservation. Among these parameters, the cooling and thawing rates, as well as the temperature of nucleation can be adjusted by a specific cryopreservation method. In this work, we reveal the optimal conditions for cryopreservation of human fibroblasts (HF), human pulmonary microvascular endothelial cells (HPMECs), amnion (aMSCs), and bone marrow stem cells (bMSCs) using an electro-freezing method and applying 2.5 %, 5 % or 10 % (v/v) dimethyl sulfoxide (Me2SO) as a cryoprotective agent. The optimal nucleation temperature for freezing of HF was -10°C for a cooling rate of 1 K·min-1 and -7.5°C for 5 K·min-1 using 5 % Me2SO. Application of a cooling rate of 5 K·min-1 and induction of the ice formation at -12°C resulted in 90 % of viable HPMECs. The aMSCs and bMSCs reflected the highest viability of 75 % after freezing using a two-step freezing protocol utilizing a cooling rate of 7.5 K·min-1 down to -30°C and 3 K·min-1 down to -80°C. The highest cell viability was observed while inducing the ice formation at -10°C for both aMSCs and bMSCs.
AB - Effective long-term storage of rare and clinically relevant cells depends on the cell type and thus requires optimization of the main process parameters involved in cryopreservation. Among these parameters, the cooling and thawing rates, as well as the temperature of nucleation can be adjusted by a specific cryopreservation method. In this work, we reveal the optimal conditions for cryopreservation of human fibroblasts (HF), human pulmonary microvascular endothelial cells (HPMECs), amnion (aMSCs), and bone marrow stem cells (bMSCs) using an electro-freezing method and applying 2.5 %, 5 % or 10 % (v/v) dimethyl sulfoxide (Me2SO) as a cryoprotective agent. The optimal nucleation temperature for freezing of HF was -10°C for a cooling rate of 1 K·min-1 and -7.5°C for 5 K·min-1 using 5 % Me2SO. Application of a cooling rate of 5 K·min-1 and induction of the ice formation at -12°C resulted in 90 % of viable HPMECs. The aMSCs and bMSCs reflected the highest viability of 75 % after freezing using a two-step freezing protocol utilizing a cooling rate of 7.5 K·min-1 down to -30°C and 3 K·min-1 down to -80°C. The highest cell viability was observed while inducing the ice formation at -10°C for both aMSCs and bMSCs.
UR - http://www.scopus.com/inward/record.url?scp=85017603076&partnerID=8YFLogxK
U2 - 10.18462/iir.cals.2016.0007
DO - 10.18462/iir.cals.2016.0007
M3 - Conference contribution
AN - SCOPUS:85017603076
T3 - Refrigeration Science and Technology
SP - 84
EP - 90
BT - IIR Workshop on Cold Applications in Life Sciences - Proceedings
T2 - 2016 IIR Workshop on Cold Applications in Life Sciences
Y2 - 8 September 2016 through 9 September 2016
ER -