Details
| Original language | English |
|---|---|
| Pages (from-to) | 144-155 |
| Number of pages | 12 |
| Journal | Dental Materials |
| Volume | 35 |
| Issue number | 1 |
| Publication status | Published - Jan 2019 |
| Externally published | Yes |
Abstract
OBJECTIVE: Dental pulp stem cells (DPSCs) can differentiate into tissue specific lineages to support dental pulp regeneration after injuries. Triethylene glycol dimethacrylate (TEGDMA) is a widely used co-monomer in restorative dentistry with adverse effects on cellular metabolism. Aim of this study was to analyze the impact of TEGDMA on the angiogenic differentiation potential of DPSCs.
METHODS: DPSCs were characterized by flow cytometry. Short-term (max. 72h) cytotoxicity of TEGDMA was assessed by MTT assay. To evaluate TEGDMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of short-term non-toxic TEGDMA concentrations (0.1mM and 0.25mM). Subsequently, angiogenic differentiation was analyzed by qRT-PCR analysis of mRNA markers and in vitro spheroid sprouting assays.
RESULTS: DPSCs treated with 0.25mM TEGDMA revealed downregulation of angiogenesis-related marker genes PECAM1 (max. 3.8-fold), VEGF-A (max. 2.4-fold) and FLT1 (max. 2.9-fold) compared to respective untreated control. In addition, a reduction of the sprouting potential of DPSCs cultured in the presence of 0.25mM TEGDMA was detectable. Larger spheroidal structures were detectable in the untreated control in comparison to cells treated with 0.25mM TEGDMA. In contrast, TEGDMA at 0.1mM was not affecting angiogenic potential in the investigated time period (up to 28 days).
SIGNIFICANCE: The results of the present study show that TEGDMA concentration dependently impair the angiogenic differentiation potential of DPSCs and may affect wound healing and the formation of granulation tissue.
Keywords
- Cell Differentiation, Cells, Cultured, Dental Pulp, Polyethylene Glycols, Polymethacrylic Acids, Stem Cells, Dental pulp stem cells, Spheroid sprouting, TEGDMA, Angiogenic differentiation, Gene expression
ASJC Scopus subject areas
- Engineering(all)
- Mechanics of Materials
- Materials Science(all)
- Dentistry(all)
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In: Dental Materials, Vol. 35, No. 1, 01.2019, p. 144-155.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Impaired angiogenic differentiation of dental pulp stem cells during exposure to the resinous monomer triethylene glycol dimethacrylate
AU - Schertl, Peter
AU - Volk, Joachim
AU - Perduns, Renke
AU - Adam, Knut
AU - Leyhausen, Gabriele
AU - Bakopoulou, Athina
AU - Geurtsen, Werner
N1 - Funding information: We thank Ms. R. Bergin, Ms. S. Taubeler-Gerling and Ms. A. Beckedorf for their technical assistance. This study was supported by a grant of the Deutsche Forschungsgemeinschaft/German National Science Foundation (DFG) (GE 455/17-1).
PY - 2019/1
Y1 - 2019/1
N2 - OBJECTIVE: Dental pulp stem cells (DPSCs) can differentiate into tissue specific lineages to support dental pulp regeneration after injuries. Triethylene glycol dimethacrylate (TEGDMA) is a widely used co-monomer in restorative dentistry with adverse effects on cellular metabolism. Aim of this study was to analyze the impact of TEGDMA on the angiogenic differentiation potential of DPSCs.METHODS: DPSCs were characterized by flow cytometry. Short-term (max. 72h) cytotoxicity of TEGDMA was assessed by MTT assay. To evaluate TEGDMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of short-term non-toxic TEGDMA concentrations (0.1mM and 0.25mM). Subsequently, angiogenic differentiation was analyzed by qRT-PCR analysis of mRNA markers and in vitro spheroid sprouting assays.RESULTS: DPSCs treated with 0.25mM TEGDMA revealed downregulation of angiogenesis-related marker genes PECAM1 (max. 3.8-fold), VEGF-A (max. 2.4-fold) and FLT1 (max. 2.9-fold) compared to respective untreated control. In addition, a reduction of the sprouting potential of DPSCs cultured in the presence of 0.25mM TEGDMA was detectable. Larger spheroidal structures were detectable in the untreated control in comparison to cells treated with 0.25mM TEGDMA. In contrast, TEGDMA at 0.1mM was not affecting angiogenic potential in the investigated time period (up to 28 days).SIGNIFICANCE: The results of the present study show that TEGDMA concentration dependently impair the angiogenic differentiation potential of DPSCs and may affect wound healing and the formation of granulation tissue.
AB - OBJECTIVE: Dental pulp stem cells (DPSCs) can differentiate into tissue specific lineages to support dental pulp regeneration after injuries. Triethylene glycol dimethacrylate (TEGDMA) is a widely used co-monomer in restorative dentistry with adverse effects on cellular metabolism. Aim of this study was to analyze the impact of TEGDMA on the angiogenic differentiation potential of DPSCs.METHODS: DPSCs were characterized by flow cytometry. Short-term (max. 72h) cytotoxicity of TEGDMA was assessed by MTT assay. To evaluate TEGDMA effects on angiogenic differentiation, DPSCs were cultivated in angiogenic differentiation medium (ADM) in the presence or absence of short-term non-toxic TEGDMA concentrations (0.1mM and 0.25mM). Subsequently, angiogenic differentiation was analyzed by qRT-PCR analysis of mRNA markers and in vitro spheroid sprouting assays.RESULTS: DPSCs treated with 0.25mM TEGDMA revealed downregulation of angiogenesis-related marker genes PECAM1 (max. 3.8-fold), VEGF-A (max. 2.4-fold) and FLT1 (max. 2.9-fold) compared to respective untreated control. In addition, a reduction of the sprouting potential of DPSCs cultured in the presence of 0.25mM TEGDMA was detectable. Larger spheroidal structures were detectable in the untreated control in comparison to cells treated with 0.25mM TEGDMA. In contrast, TEGDMA at 0.1mM was not affecting angiogenic potential in the investigated time period (up to 28 days).SIGNIFICANCE: The results of the present study show that TEGDMA concentration dependently impair the angiogenic differentiation potential of DPSCs and may affect wound healing and the formation of granulation tissue.
KW - Cell Differentiation
KW - Cells, Cultured
KW - Dental Pulp
KW - Polyethylene Glycols
KW - Polymethacrylic Acids
KW - Stem Cells
KW - Dental pulp stem cells
KW - Spheroid sprouting
KW - TEGDMA
KW - Angiogenic differentiation
KW - Gene expression
UR - http://www.scopus.com/inward/record.url?scp=85057182104&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2018.11.006
DO - 10.1016/j.dental.2018.11.006
M3 - Article
C2 - 30502225
VL - 35
SP - 144
EP - 155
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
IS - 1
ER -