Details
| Original language | English |
|---|---|
| Article number | e02218-17 |
| Journal | Applied and Environmental Microbiology |
| Volume | 84 |
| Issue number | 3 |
| Publication status | Published - 1 Feb 2018 |
| Externally published | Yes |
Abstract
Ombrotrophic peatlands are a recognized global carbon reservoir. Without restoration and peat regrowth, harvested peatlands are dramatically altered, impairing their carbon sink function, with consequences for methane turnover. Previous studies determined the impact of commercial mining on the physicochemical properties of peat and the effects on methane turnover. However, the response of the underlying microbial communities catalyzing methane production and oxidation have so far received little attention. We hypothesize that with the return of Sphagnum spp. postharvest, methane turnover potential and the corresponding microbial communities will converge in a natural and restored peatland. To address our hypothesis, we determined the potential methane production and oxidation rates in natural (as a reference), actively mined, abandoned, and restored peatlands over two consecutive years. In all sites, the methanogenic and methanotrophic population sizes were enumerated using quantitative PCR (qPCR) assays targeting the mcrA and pmoA genes, respectively. Shifts in the community composition were determined using Illumina MiSeq sequencing of the mcrA gene and a pmoA-based terminal restriction fragment length polymorphism (t-RFLP) analysis, complemented by cloning and sequence analysis of the mmoX gene. Peat mining adversely affected methane turnover potential, but the rates recovered in the restored site. The recovery in potential activity was reflected in the methanogenic and methanotrophic abundances. However, the microbial community composition was altered, being more pronounced for the methanotrophs. Overall, we observed a lag between the recovery of the methanogenic/ methanotrophic activity and the return of the corresponding microbial communities, suggesting that a longer duration (> 15 years) is needed to reverse mining-induced effects on the methane-cycling microbial communities.
Keywords
- Land use change, Methane oxidation, Methanogenesis, NifH, Nitrogen fixation, Sphagnum
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Food Science
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Environmental Science(all)
- Ecology
Sustainable Development Goals
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In: Applied and Environmental Microbiology, Vol. 84, No. 3, e02218-17, 01.02.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Impact of peat mining and restoration on methane turnover potential and methane-cycling microorganisms in a northern bog
AU - Reumer, Max
AU - Harnisz, Monika
AU - Lee, Hyo Jung
AU - Reim, Andreas
AU - Grunert, Oliver
AU - Putkinen, Anuliina
AU - Fritze, Hannu
AU - Bodelier, Paul L.E.
AU - Ho, Adrian
N1 - Publisher Copyright: © 2018 American Society for Microbiology. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Ombrotrophic peatlands are a recognized global carbon reservoir. Without restoration and peat regrowth, harvested peatlands are dramatically altered, impairing their carbon sink function, with consequences for methane turnover. Previous studies determined the impact of commercial mining on the physicochemical properties of peat and the effects on methane turnover. However, the response of the underlying microbial communities catalyzing methane production and oxidation have so far received little attention. We hypothesize that with the return of Sphagnum spp. postharvest, methane turnover potential and the corresponding microbial communities will converge in a natural and restored peatland. To address our hypothesis, we determined the potential methane production and oxidation rates in natural (as a reference), actively mined, abandoned, and restored peatlands over two consecutive years. In all sites, the methanogenic and methanotrophic population sizes were enumerated using quantitative PCR (qPCR) assays targeting the mcrA and pmoA genes, respectively. Shifts in the community composition were determined using Illumina MiSeq sequencing of the mcrA gene and a pmoA-based terminal restriction fragment length polymorphism (t-RFLP) analysis, complemented by cloning and sequence analysis of the mmoX gene. Peat mining adversely affected methane turnover potential, but the rates recovered in the restored site. The recovery in potential activity was reflected in the methanogenic and methanotrophic abundances. However, the microbial community composition was altered, being more pronounced for the methanotrophs. Overall, we observed a lag between the recovery of the methanogenic/ methanotrophic activity and the return of the corresponding microbial communities, suggesting that a longer duration (> 15 years) is needed to reverse mining-induced effects on the methane-cycling microbial communities.
AB - Ombrotrophic peatlands are a recognized global carbon reservoir. Without restoration and peat regrowth, harvested peatlands are dramatically altered, impairing their carbon sink function, with consequences for methane turnover. Previous studies determined the impact of commercial mining on the physicochemical properties of peat and the effects on methane turnover. However, the response of the underlying microbial communities catalyzing methane production and oxidation have so far received little attention. We hypothesize that with the return of Sphagnum spp. postharvest, methane turnover potential and the corresponding microbial communities will converge in a natural and restored peatland. To address our hypothesis, we determined the potential methane production and oxidation rates in natural (as a reference), actively mined, abandoned, and restored peatlands over two consecutive years. In all sites, the methanogenic and methanotrophic population sizes were enumerated using quantitative PCR (qPCR) assays targeting the mcrA and pmoA genes, respectively. Shifts in the community composition were determined using Illumina MiSeq sequencing of the mcrA gene and a pmoA-based terminal restriction fragment length polymorphism (t-RFLP) analysis, complemented by cloning and sequence analysis of the mmoX gene. Peat mining adversely affected methane turnover potential, but the rates recovered in the restored site. The recovery in potential activity was reflected in the methanogenic and methanotrophic abundances. However, the microbial community composition was altered, being more pronounced for the methanotrophs. Overall, we observed a lag between the recovery of the methanogenic/ methanotrophic activity and the return of the corresponding microbial communities, suggesting that a longer duration (> 15 years) is needed to reverse mining-induced effects on the methane-cycling microbial communities.
KW - Land use change
KW - Methane oxidation
KW - Methanogenesis
KW - NifH
KW - Nitrogen fixation
KW - Sphagnum
UR - http://www.scopus.com/inward/record.url?scp=85040666032&partnerID=8YFLogxK
U2 - 10.1128/AEM.02218-17
DO - 10.1128/AEM.02218-17
M3 - Article
C2 - 29180368
AN - SCOPUS:85040666032
VL - 84
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 3
M1 - e02218-17
ER -