Details
| Original language | English |
|---|---|
| Pages (from-to) | 473-483 |
| Number of pages | 11 |
| Journal | Plant physiology and biochemistry |
| Volume | 43 |
| Issue number | 5 |
| Publication status | Published - 1 May 2005 |
Abstract
The effects of elevated atmospheric hydrogen sulfide (H2S) levels (0.25, 0.5, and 0.75 μl l-1) have been investigated in a short-term exposure experiment (3-48:h) on the model plant Arabidopsis thaliana (L.) Heynh. in comparison to untreated control plants. The most pronounced effects of H2S fumigation could be observed on the metabolite level: The contents of the thiols cysteine and glutathione were increased up to 20- and fourfold, respectively. A direct positive correlation of the thiol contents with the H2S concentrations applied was observed. To elucidate the molecular basis for the increased thiol levels, enzyme activities, messenger RNA and protein steady-state levels of cysteine-synthesizing and degrading pathways have been determined. The enzyme activities of O-acetyl-l-serine(thiol)lyase (OAS-TL) (EC 4.2.99.8) and l-cysteine desulfhydrase (EC 4.4.1.-) proteins were not significantly higher at elevated H2S levels in comparison to untreated control plants. 3-Mercaptopyruvate sulfurtransferase (EC 2.8.1.2) activity was slightly higher after the longest H2S exposure times. Elevated H2S levels of 0.25 and 0.5 μl l-1 had promoting effects on both mRNA and protein levels of cysteine-synthesizing and degrading enzymes whereas the highest H2S concentrations caused lower levels of expression combined with mild symptoms of oxidative stress, as the consequence of its phytotoxicity. The differences in the expression of the three different OAS-TL isoforms (cytoplasmic, plastidic and mitochondrial) by H 2S were very small. Increasing concentrations of H2S and longer exposure times to H2S let to a reduction in the pool of O-acetyl-l-serine, the second precursor of cysteine, and N-acetyl-l-serine in the leaves and shoots, indicating a substrate depletion in agreement with the increased thiol levels.
Keywords
- Arabidopsis thaliana, Cysteine, Desulfhydrase, HS, O-acetyl-l-serine
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant physiology and biochemistry, Vol. 43, No. 5, 01.05.2005, p. 473-483.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Impact of elevated H2S on metabolite levels, activity of enzymes and expression of genes involved in cysteine metabolism
AU - Riemenschneider, Anja
AU - Nikiforova, Victoria
AU - Hoefgen, Rainer
AU - De Kok, Luit J.
AU - Papenbrock, Jutta
N1 - Funding information: We would like to thank P. von Trzebiatowski and J. Volker for their excellent technical assistance. The contribution of Mrs. C.E.E. Stuiver to the project is gratefully acknowledged. Initial H 2 S fumigation experiments were done by Dr. P. Burandt, University of Hannover. The measurements of the sulfurtransferase activities were performed by Dr. M. Bauer. We are thankful to Professor Dr. A. Schmidt, University of Hannover, for stimulating discussions. The work was supported financially by grants of the Deutsche Forschungsgemeinschaft (PA 764/1-3, PA 764/2-1, SCHM 307/15-3), the EU COST action 829, the Max Planck Society and the EU fp5 project QLRT-2000-00103.
PY - 2005/5/1
Y1 - 2005/5/1
N2 - The effects of elevated atmospheric hydrogen sulfide (H2S) levels (0.25, 0.5, and 0.75 μl l-1) have been investigated in a short-term exposure experiment (3-48:h) on the model plant Arabidopsis thaliana (L.) Heynh. in comparison to untreated control plants. The most pronounced effects of H2S fumigation could be observed on the metabolite level: The contents of the thiols cysteine and glutathione were increased up to 20- and fourfold, respectively. A direct positive correlation of the thiol contents with the H2S concentrations applied was observed. To elucidate the molecular basis for the increased thiol levels, enzyme activities, messenger RNA and protein steady-state levels of cysteine-synthesizing and degrading pathways have been determined. The enzyme activities of O-acetyl-l-serine(thiol)lyase (OAS-TL) (EC 4.2.99.8) and l-cysteine desulfhydrase (EC 4.4.1.-) proteins were not significantly higher at elevated H2S levels in comparison to untreated control plants. 3-Mercaptopyruvate sulfurtransferase (EC 2.8.1.2) activity was slightly higher after the longest H2S exposure times. Elevated H2S levels of 0.25 and 0.5 μl l-1 had promoting effects on both mRNA and protein levels of cysteine-synthesizing and degrading enzymes whereas the highest H2S concentrations caused lower levels of expression combined with mild symptoms of oxidative stress, as the consequence of its phytotoxicity. The differences in the expression of the three different OAS-TL isoforms (cytoplasmic, plastidic and mitochondrial) by H 2S were very small. Increasing concentrations of H2S and longer exposure times to H2S let to a reduction in the pool of O-acetyl-l-serine, the second precursor of cysteine, and N-acetyl-l-serine in the leaves and shoots, indicating a substrate depletion in agreement with the increased thiol levels.
AB - The effects of elevated atmospheric hydrogen sulfide (H2S) levels (0.25, 0.5, and 0.75 μl l-1) have been investigated in a short-term exposure experiment (3-48:h) on the model plant Arabidopsis thaliana (L.) Heynh. in comparison to untreated control plants. The most pronounced effects of H2S fumigation could be observed on the metabolite level: The contents of the thiols cysteine and glutathione were increased up to 20- and fourfold, respectively. A direct positive correlation of the thiol contents with the H2S concentrations applied was observed. To elucidate the molecular basis for the increased thiol levels, enzyme activities, messenger RNA and protein steady-state levels of cysteine-synthesizing and degrading pathways have been determined. The enzyme activities of O-acetyl-l-serine(thiol)lyase (OAS-TL) (EC 4.2.99.8) and l-cysteine desulfhydrase (EC 4.4.1.-) proteins were not significantly higher at elevated H2S levels in comparison to untreated control plants. 3-Mercaptopyruvate sulfurtransferase (EC 2.8.1.2) activity was slightly higher after the longest H2S exposure times. Elevated H2S levels of 0.25 and 0.5 μl l-1 had promoting effects on both mRNA and protein levels of cysteine-synthesizing and degrading enzymes whereas the highest H2S concentrations caused lower levels of expression combined with mild symptoms of oxidative stress, as the consequence of its phytotoxicity. The differences in the expression of the three different OAS-TL isoforms (cytoplasmic, plastidic and mitochondrial) by H 2S were very small. Increasing concentrations of H2S and longer exposure times to H2S let to a reduction in the pool of O-acetyl-l-serine, the second precursor of cysteine, and N-acetyl-l-serine in the leaves and shoots, indicating a substrate depletion in agreement with the increased thiol levels.
KW - Arabidopsis thaliana
KW - Cysteine
KW - Desulfhydrase
KW - HS
KW - O-acetyl-l-serine
UR - http://www.scopus.com/inward/record.url?scp=20444414185&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2005.04.001
DO - 10.1016/j.plaphy.2005.04.001
M3 - Article
C2 - 15914014
AN - SCOPUS:20444414185
VL - 43
SP - 473
EP - 483
JO - Plant physiology and biochemistry
JF - Plant physiology and biochemistry
SN - 0981-9428
IS - 5
ER -