Details
| Original language | English |
|---|---|
| Article number | 668 |
| Journal | Foods |
| Volume | 12 |
| Issue number | 3 |
| Publication status | Published - 3 Feb 2023 |
Abstract
The quality and harvest of essential oils depend on a large number of factors, most of which are hard to control in an open-field environment. Therefore, Basidiomycota have gained attention as a source for biotechnologically produced terpenoids. The basidiomycete Cerrena unicolor (Cun) was cultivated in submerged culture, and the production of sesquiterpenoids was analyzed via stir bar sorptive extraction (SBSE), followed by thermo-desorption gas chromatography coupled with mass spectrometry (TDS-GC-MS). Identification of aroma-active sesquiterpenoids was supported by GC, coupled with an olfactory detection port (ODP). Following the ideal of a circular bioeconomy, Cun was submerged (up-scalable) cultivated, and supplemented with a variety of food industrial side-streams. The effects of the different supplementations and of pure fatty acids were evaluated by liquid extraction and analysis of the terpenoids via GC-MS. As sesquiterpenoid production was enhanced by the most by lipid-rich side-streams, a cultivation with 13C-labeled acetate was conducted. Data confirmed that lipid-rich side-streams enhanced the sesquiterpene production through an increased acetyl-CoA pool.
Keywords
- Basidiomycota, bioeconomy, Cerrena unicolor, medium supplementation, rapeseed press cake, sesquiterpene synthesis, side-streams
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Immunology and Microbiology(all)
- Microbiology
- Social Sciences(all)
- Health(social science)
- Health Professions(all)
- Health Professions (miscellaneous)
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Foods, Vol. 12, No. 3, 668, 03.02.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Impact of Agro-Industrial Side-Streams on Sesquiterpene Production by Submerged Cultured Cerrena unicolor
AU - Püth, Nils
AU - Ersoy, Franziska
AU - Berger, Ralf G.
AU - Krings, Ulrich
N1 - Funding Information: The APC was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2023/2/3
Y1 - 2023/2/3
N2 - The quality and harvest of essential oils depend on a large number of factors, most of which are hard to control in an open-field environment. Therefore, Basidiomycota have gained attention as a source for biotechnologically produced terpenoids. The basidiomycete Cerrena unicolor (Cun) was cultivated in submerged culture, and the production of sesquiterpenoids was analyzed via stir bar sorptive extraction (SBSE), followed by thermo-desorption gas chromatography coupled with mass spectrometry (TDS-GC-MS). Identification of aroma-active sesquiterpenoids was supported by GC, coupled with an olfactory detection port (ODP). Following the ideal of a circular bioeconomy, Cun was submerged (up-scalable) cultivated, and supplemented with a variety of food industrial side-streams. The effects of the different supplementations and of pure fatty acids were evaluated by liquid extraction and analysis of the terpenoids via GC-MS. As sesquiterpenoid production was enhanced by the most by lipid-rich side-streams, a cultivation with 13C-labeled acetate was conducted. Data confirmed that lipid-rich side-streams enhanced the sesquiterpene production through an increased acetyl-CoA pool.
AB - The quality and harvest of essential oils depend on a large number of factors, most of which are hard to control in an open-field environment. Therefore, Basidiomycota have gained attention as a source for biotechnologically produced terpenoids. The basidiomycete Cerrena unicolor (Cun) was cultivated in submerged culture, and the production of sesquiterpenoids was analyzed via stir bar sorptive extraction (SBSE), followed by thermo-desorption gas chromatography coupled with mass spectrometry (TDS-GC-MS). Identification of aroma-active sesquiterpenoids was supported by GC, coupled with an olfactory detection port (ODP). Following the ideal of a circular bioeconomy, Cun was submerged (up-scalable) cultivated, and supplemented with a variety of food industrial side-streams. The effects of the different supplementations and of pure fatty acids were evaluated by liquid extraction and analysis of the terpenoids via GC-MS. As sesquiterpenoid production was enhanced by the most by lipid-rich side-streams, a cultivation with 13C-labeled acetate was conducted. Data confirmed that lipid-rich side-streams enhanced the sesquiterpene production through an increased acetyl-CoA pool.
KW - Basidiomycota
KW - bioeconomy
KW - Cerrena unicolor
KW - medium supplementation
KW - rapeseed press cake
KW - sesquiterpene synthesis
KW - side-streams
UR - http://www.scopus.com/inward/record.url?scp=85147827374&partnerID=8YFLogxK
U2 - 10.3390/foods12030668
DO - 10.3390/foods12030668
M3 - Article
AN - SCOPUS:85147827374
VL - 12
JO - Foods
JF - Foods
IS - 3
M1 - 668
ER -