Details
| Original language | English |
|---|---|
| Pages (from-to) | 639-45 |
| Number of pages | 7 |
| Journal | Acta histochemica |
| Volume | 116 |
| Issue number | 4 |
| Publication status | Published - May 2014 |
Abstract
Based on immunohistochemistry, the study demonstrates the varying distribution and reaction intensity of connexins (Cx26 [chicken 31sim], 30 [chicken 31], 31, 32, 43, 45) in the developing feather follicle of the chicken (White Leghorn). The different embryonal stages were identified according to the normal table of Hamburger and Hamilton (1951). The development of the feather follicle complex is closely related to skin layer development, making use of the controlling function of connexins. This was evident during feather follicle differentiation, based on communication between ectomesodermal (fibroblasts) and ectodermal cells (developing epidermis), but also by the subsequent separation of the two cell line types related to their connexin-dependent differentiation degree. With the increase in mesenchymal cell numbers during feather placode development, the multiple connexins Cx26 [chicken 31sim] and 43, supported by Cx30 [chicken 31], 31 and 32, were increasingly activating the fibroblast concentrations as related to epidermal follicle buds, the specific follicle structure, the endothelial cells of capillaries and larger blood vessels, as well as the collagen fiber production and the growing feather musculature shortly before hatching; Cx45 could not be demonstrated. In conclusion, it seems that connexin expression is not only coupled to the origin of embryonic cells, but also connected with tissue formation before the follicle system can be formed.
Keywords
- Animals, Cell Differentiation/physiology, Cell Line, Chick Embryo, Chickens, Connexins/metabolism, Feathers, Fibroblasts/metabolism, Immunohistochemistry/methods, Skin/embryology
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In: Acta histochemica, Vol. 116, No. 4, 05.2014, p. 639-45.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Immunohistochemical demonstration of connexins in the developing feather follicle of the chicken
AU - Meyer, Wilfried
AU - Oberthuer, Anna
AU - Ngezahayo, Anaclet
AU - Neumann, Ulrich
AU - Jacob, Ralf
N1 - Funding information: This work was supported by the Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany (Graduiertenkolleg 1216 and Sonderforschungsbereich 593 to RJ) .
PY - 2014/5
Y1 - 2014/5
N2 - Based on immunohistochemistry, the study demonstrates the varying distribution and reaction intensity of connexins (Cx26 [chicken 31sim], 30 [chicken 31], 31, 32, 43, 45) in the developing feather follicle of the chicken (White Leghorn). The different embryonal stages were identified according to the normal table of Hamburger and Hamilton (1951). The development of the feather follicle complex is closely related to skin layer development, making use of the controlling function of connexins. This was evident during feather follicle differentiation, based on communication between ectomesodermal (fibroblasts) and ectodermal cells (developing epidermis), but also by the subsequent separation of the two cell line types related to their connexin-dependent differentiation degree. With the increase in mesenchymal cell numbers during feather placode development, the multiple connexins Cx26 [chicken 31sim] and 43, supported by Cx30 [chicken 31], 31 and 32, were increasingly activating the fibroblast concentrations as related to epidermal follicle buds, the specific follicle structure, the endothelial cells of capillaries and larger blood vessels, as well as the collagen fiber production and the growing feather musculature shortly before hatching; Cx45 could not be demonstrated. In conclusion, it seems that connexin expression is not only coupled to the origin of embryonic cells, but also connected with tissue formation before the follicle system can be formed.
AB - Based on immunohistochemistry, the study demonstrates the varying distribution and reaction intensity of connexins (Cx26 [chicken 31sim], 30 [chicken 31], 31, 32, 43, 45) in the developing feather follicle of the chicken (White Leghorn). The different embryonal stages were identified according to the normal table of Hamburger and Hamilton (1951). The development of the feather follicle complex is closely related to skin layer development, making use of the controlling function of connexins. This was evident during feather follicle differentiation, based on communication between ectomesodermal (fibroblasts) and ectodermal cells (developing epidermis), but also by the subsequent separation of the two cell line types related to their connexin-dependent differentiation degree. With the increase in mesenchymal cell numbers during feather placode development, the multiple connexins Cx26 [chicken 31sim] and 43, supported by Cx30 [chicken 31], 31 and 32, were increasingly activating the fibroblast concentrations as related to epidermal follicle buds, the specific follicle structure, the endothelial cells of capillaries and larger blood vessels, as well as the collagen fiber production and the growing feather musculature shortly before hatching; Cx45 could not be demonstrated. In conclusion, it seems that connexin expression is not only coupled to the origin of embryonic cells, but also connected with tissue formation before the follicle system can be formed.
KW - Animals
KW - Cell Differentiation/physiology
KW - Cell Line
KW - Chick Embryo
KW - Chickens
KW - Connexins/metabolism
KW - Feathers
KW - Fibroblasts/metabolism
KW - Immunohistochemistry/methods
KW - Skin/embryology
U2 - 10.1016/j.acthis.2013.11.016
DO - 10.1016/j.acthis.2013.11.016
M3 - Article
C2 - 24345685
VL - 116
SP - 639
EP - 645
JO - Acta histochemica
JF - Acta histochemica
SN - 0065-1281
IS - 4
ER -