Details
| Original language | English |
|---|---|
| Pages (from-to) | 301-305 |
| Number of pages | 5 |
| Journal | Analytica chimica acta |
| Volume | 309 |
| Issue number | 1-3 |
| Publication status | Published - 20 Jun 1995 |
| Externally published | Yes |
Abstract
A flow-injection analysis (FIA) system with an integrated immunobased elution assay was developed for the on-line determination of biotechnological products. This paper describes its application for tissue-type plasminogen activator (rt-PA) and recombinant antithrombin III (r-AT III). The short response time of the immunochemical system allows its use for measurement and control of biotechnological production processes. Immobilized polyclonal antibodies were found to bind rt-PA in the cartridge better than monoclonal antibodies. Sepharose-4B was found to be the best support for the antibodies. The cartridge with immobilized antibodies can be prepared easily, has a high reliability and is stable for more than two weeks. The total time necessary for one assay cycle is in the range of 6-8 min, depending on the conditions. The whole procedure is controlled by a personal computer. The FIA system can be used for automatic analysis as a stand-alone version with a frequency of 7 samples per hour. The standard operation range is from 500 μg/ml down to 2 μg/ml. A comparison with the conventional microtiter ELISA assay shows that this assay can be applied for an accurate determination of undiluted cultivation samples.
Keywords
- Flow injection, Immunoassay, Process analysis
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Analytica chimica acta, Vol. 309, No. 1-3, 20.06.1995, p. 301-305.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Immunobased elution assay for process control
AU - Beyer, Kerstin
AU - Reinecke, Martin
AU - Noe, Wolfgang
AU - Scheper, Thomas
PY - 1995/6/20
Y1 - 1995/6/20
N2 - A flow-injection analysis (FIA) system with an integrated immunobased elution assay was developed for the on-line determination of biotechnological products. This paper describes its application for tissue-type plasminogen activator (rt-PA) and recombinant antithrombin III (r-AT III). The short response time of the immunochemical system allows its use for measurement and control of biotechnological production processes. Immobilized polyclonal antibodies were found to bind rt-PA in the cartridge better than monoclonal antibodies. Sepharose-4B was found to be the best support for the antibodies. The cartridge with immobilized antibodies can be prepared easily, has a high reliability and is stable for more than two weeks. The total time necessary for one assay cycle is in the range of 6-8 min, depending on the conditions. The whole procedure is controlled by a personal computer. The FIA system can be used for automatic analysis as a stand-alone version with a frequency of 7 samples per hour. The standard operation range is from 500 μg/ml down to 2 μg/ml. A comparison with the conventional microtiter ELISA assay shows that this assay can be applied for an accurate determination of undiluted cultivation samples.
AB - A flow-injection analysis (FIA) system with an integrated immunobased elution assay was developed for the on-line determination of biotechnological products. This paper describes its application for tissue-type plasminogen activator (rt-PA) and recombinant antithrombin III (r-AT III). The short response time of the immunochemical system allows its use for measurement and control of biotechnological production processes. Immobilized polyclonal antibodies were found to bind rt-PA in the cartridge better than monoclonal antibodies. Sepharose-4B was found to be the best support for the antibodies. The cartridge with immobilized antibodies can be prepared easily, has a high reliability and is stable for more than two weeks. The total time necessary for one assay cycle is in the range of 6-8 min, depending on the conditions. The whole procedure is controlled by a personal computer. The FIA system can be used for automatic analysis as a stand-alone version with a frequency of 7 samples per hour. The standard operation range is from 500 μg/ml down to 2 μg/ml. A comparison with the conventional microtiter ELISA assay shows that this assay can be applied for an accurate determination of undiluted cultivation samples.
KW - Flow injection
KW - Immunoassay
KW - Process analysis
UR - http://www.scopus.com/inward/record.url?scp=0029008499&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(95)00092-E
DO - 10.1016/0003-2670(95)00092-E
M3 - Article
AN - SCOPUS:0029008499
VL - 309
SP - 301
EP - 305
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - 1-3
ER -