Details
| Original language | English |
|---|---|
| Pages (from-to) | 115-144 |
| Number of pages | 30 |
| Journal | Journal of biotechnology |
| Volume | 25 |
| Issue number | 1-2 |
| Publication status | Published - Aug 1992 |
Abstract
In this article, the applications of immunoanalysis and flow cytometry for research and process monitoring in biotechnology are discussed. Brief reviews of the two analytical methods are followed by descriptions of actual applications in various areas of biotechnology. In the case of immunoanalysis, emphasis is placed on systems for on-line bioprocess monitoring, and examples are given for a thermostable pullulanase, a mouse IgG, and antithrombin III. Although flow cytometry is not currently an on-line analytical technique, its value as an off-line method is illustrated by examples of the measurement of shear stress effects, lipid content, and sterol content.
Keywords
- Bioprocess monitoring, Flow injection analysis, Immunoassay, Laser flow cytometry, On-line analysis
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 25, No. 1-2, 08.1992, p. 115-144.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Immuno- and flow cytometric analytical methods for biotechnological research and process monitoring
AU - Degelau, A.
AU - Freitag, R.
AU - Linz, F.
AU - Middendorf, C.
AU - Scheper, T.
AU - Bley, T.
AU - Müller, S.
AU - Stoll, P.
AU - Reardon, K. F.
N1 - Funding information: The authors gratefully acknowledge the support of the DFG and the BMFT (Forschungsschwerpunkt: 'Grundlagen der Bioprozesstechnik') for their cooperation.
PY - 1992/8
Y1 - 1992/8
N2 - In this article, the applications of immunoanalysis and flow cytometry for research and process monitoring in biotechnology are discussed. Brief reviews of the two analytical methods are followed by descriptions of actual applications in various areas of biotechnology. In the case of immunoanalysis, emphasis is placed on systems for on-line bioprocess monitoring, and examples are given for a thermostable pullulanase, a mouse IgG, and antithrombin III. Although flow cytometry is not currently an on-line analytical technique, its value as an off-line method is illustrated by examples of the measurement of shear stress effects, lipid content, and sterol content.
AB - In this article, the applications of immunoanalysis and flow cytometry for research and process monitoring in biotechnology are discussed. Brief reviews of the two analytical methods are followed by descriptions of actual applications in various areas of biotechnology. In the case of immunoanalysis, emphasis is placed on systems for on-line bioprocess monitoring, and examples are given for a thermostable pullulanase, a mouse IgG, and antithrombin III. Although flow cytometry is not currently an on-line analytical technique, its value as an off-line method is illustrated by examples of the measurement of shear stress effects, lipid content, and sterol content.
KW - Bioprocess monitoring
KW - Flow injection analysis
KW - Immunoassay
KW - Laser flow cytometry
KW - On-line analysis
UR - http://www.scopus.com/inward/record.url?scp=0026726386&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(92)90112-M
DO - 10.1016/0168-1656(92)90112-M
M3 - Article
C2 - 1368458
AN - SCOPUS:0026726386
VL - 25
SP - 115
EP - 144
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 1-2
ER -