Loading [MathJax]/extensions/tex2jax.js

Imaging single glycans

Research output: Contribution to journalArticleResearchpeer review

Authors

  • X. Wu
  • M. Delbianco
  • K. Anggara
  • T. Michnowicz

External Research Organisations

  • Max Planck Institute for Solid State Research (MPI-FKF)
  • Max Planck Institute of Colloids and Interfaces
  • University of Oxford
  • Technische Universität Braunschweig
  • Freie Universität Berlin (FU Berlin)
  • École polytechnique fédérale de Lausanne (EPFL)
Plum Print visual indicator of research metrics
  • Citations
    • Citation Indexes: 82
  • Captures
    • Readers: 221
  • Mentions
    • News Mentions: 2
  • Social Media
    • Shares, Likes & Comments: 238
see details

Details

Original languageEnglish
Pages (from-to)375-378
Number of pages4
JournalNATURE
Volume582
Issue number7812
Early online date17 Jun 2020
Publication statusPublished - 18 Jun 2020
Externally publishedYes

Abstract

Imaging of biomolecules guides our understanding of their diverse structures and functions1,2. Real-space imaging at sub-nanometre resolution using cryo-electron microscopy has provided key insights into proteins and their assemblies3,4. Direct molecular imaging of glycans—the predominant biopolymers on Earth, with a plethora of structural and biological functions5—has not been possible so far6. The inherent glycan complexity and backbone flexibility require single-molecule approaches for real-space imaging. At present, glycan characterization often relies on a combination of mass spectrometry and nuclear magnetic resonance imaging to provide insights into size, sequence, branching and connectivity, and therefore requires structure reconstruction from indirect information7–9. Here we show direct imaging of single glycan molecules that are isolated by mass-selective, soft-landing electrospray ion beam deposition and imaged by low-temperature scanning tunnelling microscopy10. The sub-nanometre resolution of the technique enables the visualization of glycan connectivity and discrimination between regioisomers. Direct glycan imaging is an important step towards a better understanding of the structure of carbohydrates.

ASJC Scopus subject areas

Cite this

Imaging single glycans. / Wu, X.; Delbianco, M.; Anggara, K. et al.
In: NATURE, Vol. 582, No. 7812, 18.06.2020, p. 375-378.

Research output: Contribution to journalArticleResearchpeer review

Wu, X, Delbianco, M, Anggara, K, Michnowicz, T, Pardo-Vargas, A, Bharate, P, Sen, S, Pristl, M, Rauschenbach, S, Schlickum, U, Abb, S, Seeberger, PH & Kern, K 2020, 'Imaging single glycans', NATURE, vol. 582, no. 7812, pp. 375-378. https://doi.org/10.1038/s41586-020-2362-1
Wu, X., Delbianco, M., Anggara, K., Michnowicz, T., Pardo-Vargas, A., Bharate, P., Sen, S., Pristl, M., Rauschenbach, S., Schlickum, U., Abb, S., Seeberger, P. H., & Kern, K. (2020). Imaging single glycans. NATURE, 582(7812), 375-378. https://doi.org/10.1038/s41586-020-2362-1
Wu X, Delbianco M, Anggara K, Michnowicz T, Pardo-Vargas A, Bharate P et al. Imaging single glycans. NATURE. 2020 Jun 18;582(7812):375-378. Epub 2020 Jun 17. doi: 10.1038/s41586-020-2362-1
Wu, X. ; Delbianco, M. ; Anggara, K. et al. / Imaging single glycans. In: NATURE. 2020 ; Vol. 582, No. 7812. pp. 375-378.
Download
@article{4ac4885d95664751a2465599b006dbfe,
title = "Imaging single glycans",
abstract = "Imaging of biomolecules guides our understanding of their diverse structures and functions1,2. Real-space imaging at sub-nanometre resolution using cryo-electron microscopy has provided key insights into proteins and their assemblies3,4. Direct molecular imaging of glycans—the predominant biopolymers on Earth, with a plethora of structural and biological functions5—has not been possible so far6. The inherent glycan complexity and backbone flexibility require single-molecule approaches for real-space imaging. At present, glycan characterization often relies on a combination of mass spectrometry and nuclear magnetic resonance imaging to provide insights into size, sequence, branching and connectivity, and therefore requires structure reconstruction from indirect information7–9. Here we show direct imaging of single glycan molecules that are isolated by mass-selective, soft-landing electrospray ion beam deposition and imaged by low-temperature scanning tunnelling microscopy10. The sub-nanometre resolution of the technique enables the visualization of glycan connectivity and discrimination between regioisomers. Direct glycan imaging is an important step towards a better understanding of the structure of carbohydrates.",
author = "X. Wu and M. Delbianco and K. Anggara and T. Michnowicz and A. Pardo-Vargas and P. Bharate and S. Sen and M. Pristl and S. Rauschenbach and U. Schlickum and S. Abb and Seeberger, {P. H.} and K. Kern",
note = "Funding Information: Acknowledgements We thank the Max Planck Society and Alexander von Humboldt Foundation for financial support. M.D. thanks the Minerva Fast Track Program and the MPG-FhG Cooperation Project Glyco3Dysplay. The authors acknowledge the Emmy-Noether-Program of the Deutsche Forschungsgemeinschaft.",
year = "2020",
month = jun,
day = "18",
doi = "10.1038/s41586-020-2362-1",
language = "English",
volume = "582",
pages = "375--378",
journal = "NATURE",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7812",

}

Download

TY - JOUR

T1 - Imaging single glycans

AU - Wu, X.

AU - Delbianco, M.

AU - Anggara, K.

AU - Michnowicz, T.

AU - Pardo-Vargas, A.

AU - Bharate, P.

AU - Sen, S.

AU - Pristl, M.

AU - Rauschenbach, S.

AU - Schlickum, U.

AU - Abb, S.

AU - Seeberger, P. H.

AU - Kern, K.

N1 - Funding Information: Acknowledgements We thank the Max Planck Society and Alexander von Humboldt Foundation for financial support. M.D. thanks the Minerva Fast Track Program and the MPG-FhG Cooperation Project Glyco3Dysplay. The authors acknowledge the Emmy-Noether-Program of the Deutsche Forschungsgemeinschaft.

PY - 2020/6/18

Y1 - 2020/6/18

N2 - Imaging of biomolecules guides our understanding of their diverse structures and functions1,2. Real-space imaging at sub-nanometre resolution using cryo-electron microscopy has provided key insights into proteins and their assemblies3,4. Direct molecular imaging of glycans—the predominant biopolymers on Earth, with a plethora of structural and biological functions5—has not been possible so far6. The inherent glycan complexity and backbone flexibility require single-molecule approaches for real-space imaging. At present, glycan characterization often relies on a combination of mass spectrometry and nuclear magnetic resonance imaging to provide insights into size, sequence, branching and connectivity, and therefore requires structure reconstruction from indirect information7–9. Here we show direct imaging of single glycan molecules that are isolated by mass-selective, soft-landing electrospray ion beam deposition and imaged by low-temperature scanning tunnelling microscopy10. The sub-nanometre resolution of the technique enables the visualization of glycan connectivity and discrimination between regioisomers. Direct glycan imaging is an important step towards a better understanding of the structure of carbohydrates.

AB - Imaging of biomolecules guides our understanding of their diverse structures and functions1,2. Real-space imaging at sub-nanometre resolution using cryo-electron microscopy has provided key insights into proteins and their assemblies3,4. Direct molecular imaging of glycans—the predominant biopolymers on Earth, with a plethora of structural and biological functions5—has not been possible so far6. The inherent glycan complexity and backbone flexibility require single-molecule approaches for real-space imaging. At present, glycan characterization often relies on a combination of mass spectrometry and nuclear magnetic resonance imaging to provide insights into size, sequence, branching and connectivity, and therefore requires structure reconstruction from indirect information7–9. Here we show direct imaging of single glycan molecules that are isolated by mass-selective, soft-landing electrospray ion beam deposition and imaged by low-temperature scanning tunnelling microscopy10. The sub-nanometre resolution of the technique enables the visualization of glycan connectivity and discrimination between regioisomers. Direct glycan imaging is an important step towards a better understanding of the structure of carbohydrates.

UR - http://www.scopus.com/inward/record.url?scp=85086584571&partnerID=8YFLogxK

U2 - 10.1038/s41586-020-2362-1

DO - 10.1038/s41586-020-2362-1

M3 - Article

C2 - 32555487

AN - SCOPUS:85086584571

VL - 582

SP - 375

EP - 378

JO - NATURE

JF - NATURE

SN - 0028-0836

IS - 7812

ER -