Details
| Original language | English |
|---|---|
| Title of host publication | Photonic Therapeutics and Diagnostics IX |
| Publication status | Published - 8 Mar 2013 |
| Externally published | Yes |
| Event | Photonic Therapeutics and Diagnostics IX - San Francisco, CA, United States Duration: 2 Feb 2013 → 7 Feb 2013 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 8565 |
| ISSN (Print) | 1605-7422 |
Abstract
New optical techniques have the potential to fill the gap between radiological and microscopic approaches to assess the lung's internal structure. Since its quantitative assessment requires unbiased sampling and measurement principles, imaging of the whole lung with sufficient resolution for visualizing details is important. To address this request, we applied scanning laser optical tomography (SLOT) for the three dimensional imaging of mouse lung ex vivo. SLOT is a highly efficient flourescence and transmission microscopy technique allowing for 3D imaging of specimen of sizes up to several millimeters. Previously fixed lung lobes and whole lungs were optically cleared and subsequently imaged with SLOT while making use of intrinsic contrast mechanisms like absorption and autofluorescence. Imaging of airways, blood vessels and parenchyma is demonstrated. Volumetric SLOT datasets of the lung's internal structure can be analyzed in any preferred planar orientation. Moreover, the sample preparation preserves microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, SLOT is a useful technique to visualize and survey the internal structure of mouse lung at different scales and with various contrast mechanisms. Potential applications of SLOT in lung research are e.g. quantitative phenotype analysis of mouse models of human lung disease in combination with stereological methods.
Keywords
- 3D fluorescence microscopy, Acini, Airways, Alveoli, Blood vessels, Optical projection tomography
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Sustainable Development Goals
Cite this
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Photonic Therapeutics and Diagnostics IX. 2013. 856535 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8565).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Imaging of the mouse lung with scanning laser optical tomography (SLOT)
AU - Heidrich, Marko
AU - Kellner, Manuela
AU - Beigel, Rebecca
AU - Lorbeer, Raoul Amadeus
AU - Knudsen, Lars
AU - Heisterkamp, Alexander
AU - Ripken, Tammo
AU - Ochs, Matthias
AU - Kühnel, Mark Philipp
AU - Meyer, Heiko
PY - 2013/3/8
Y1 - 2013/3/8
N2 - New optical techniques have the potential to fill the gap between radiological and microscopic approaches to assess the lung's internal structure. Since its quantitative assessment requires unbiased sampling and measurement principles, imaging of the whole lung with sufficient resolution for visualizing details is important. To address this request, we applied scanning laser optical tomography (SLOT) for the three dimensional imaging of mouse lung ex vivo. SLOT is a highly efficient flourescence and transmission microscopy technique allowing for 3D imaging of specimen of sizes up to several millimeters. Previously fixed lung lobes and whole lungs were optically cleared and subsequently imaged with SLOT while making use of intrinsic contrast mechanisms like absorption and autofluorescence. Imaging of airways, blood vessels and parenchyma is demonstrated. Volumetric SLOT datasets of the lung's internal structure can be analyzed in any preferred planar orientation. Moreover, the sample preparation preserves microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, SLOT is a useful technique to visualize and survey the internal structure of mouse lung at different scales and with various contrast mechanisms. Potential applications of SLOT in lung research are e.g. quantitative phenotype analysis of mouse models of human lung disease in combination with stereological methods.
AB - New optical techniques have the potential to fill the gap between radiological and microscopic approaches to assess the lung's internal structure. Since its quantitative assessment requires unbiased sampling and measurement principles, imaging of the whole lung with sufficient resolution for visualizing details is important. To address this request, we applied scanning laser optical tomography (SLOT) for the three dimensional imaging of mouse lung ex vivo. SLOT is a highly efficient flourescence and transmission microscopy technique allowing for 3D imaging of specimen of sizes up to several millimeters. Previously fixed lung lobes and whole lungs were optically cleared and subsequently imaged with SLOT while making use of intrinsic contrast mechanisms like absorption and autofluorescence. Imaging of airways, blood vessels and parenchyma is demonstrated. Volumetric SLOT datasets of the lung's internal structure can be analyzed in any preferred planar orientation. Moreover, the sample preparation preserves microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, SLOT is a useful technique to visualize and survey the internal structure of mouse lung at different scales and with various contrast mechanisms. Potential applications of SLOT in lung research are e.g. quantitative phenotype analysis of mouse models of human lung disease in combination with stereological methods.
KW - 3D fluorescence microscopy
KW - Acini
KW - Airways
KW - Alveoli
KW - Blood vessels
KW - Optical projection tomography
UR - http://www.scopus.com/inward/record.url?scp=84878208372&partnerID=8YFLogxK
U2 - 10.1117/12.2003955
DO - 10.1117/12.2003955
M3 - Conference contribution
AN - SCOPUS:84878208372
SN - 9780819493347
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photonic Therapeutics and Diagnostics IX
T2 - Photonic Therapeutics and Diagnostics IX
Y2 - 2 February 2013 through 7 February 2013
ER -