Details
| Original language | English |
|---|---|
| Pages (from-to) | 6777-6780 |
| Number of pages | 4 |
| Journal | Chemical communications |
| Volume | 52 |
| Issue number | 41 |
| Publication status | Published - 1 Jan 2016 |
Abstract
A gene cluster responsible for the biosynthesis of squalestatin S1 (SQS1, 1) was identified by full genome sequencing of two SQS1-producing ascomycetes: Phoma sp. C2932 and unidentified fungus MF5453. A transformation protocol was established and a subsequent knockout of one PKS gene from the cluster led to loss of SQS1 production and enhanced concentration of an SQS1 precursor. An acyltransferase gene from the cluster was expressed in E. coli and the expressed protein MfM4 shown to be responsible for loading acyl groups from CoA onto the squalestatin core as the final step of biosynthesis. MfM4 appears to have a broad substrate selectivity for its acyl CoA substrate, allowing the in vitro synthesis of novel squalestatins.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Ceramics and Composites
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Materials Science(all)
- Materials Chemistry
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In: Chemical communications, Vol. 52, No. 41, 01.01.2016, p. 6777-6780.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Identification of genes encoding squalestatin S1 biosynthesis and
T2 - In vitro production of new squalestatin analogues
AU - Bonsch, B.
AU - Belt, V.
AU - Bartel, C.
AU - Duensing, N.
AU - Koziol, M.
AU - Lazarus, C. M.
AU - Bailey, A. M.
AU - Simpson, T. J.
AU - Cox, R. J.
N1 - Funding information: We thank EPSRC (EP/F066104/1), Leibniz Universitat Hannover and DFG (INST 187/621) for funding LCMS instruments. CB Thanks the MINAS programme of Lower Saxony for funding. We thank the University of Bristol Genomics facility for Illumina genome sequencing.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - A gene cluster responsible for the biosynthesis of squalestatin S1 (SQS1, 1) was identified by full genome sequencing of two SQS1-producing ascomycetes: Phoma sp. C2932 and unidentified fungus MF5453. A transformation protocol was established and a subsequent knockout of one PKS gene from the cluster led to loss of SQS1 production and enhanced concentration of an SQS1 precursor. An acyltransferase gene from the cluster was expressed in E. coli and the expressed protein MfM4 shown to be responsible for loading acyl groups from CoA onto the squalestatin core as the final step of biosynthesis. MfM4 appears to have a broad substrate selectivity for its acyl CoA substrate, allowing the in vitro synthesis of novel squalestatins.
AB - A gene cluster responsible for the biosynthesis of squalestatin S1 (SQS1, 1) was identified by full genome sequencing of two SQS1-producing ascomycetes: Phoma sp. C2932 and unidentified fungus MF5453. A transformation protocol was established and a subsequent knockout of one PKS gene from the cluster led to loss of SQS1 production and enhanced concentration of an SQS1 precursor. An acyltransferase gene from the cluster was expressed in E. coli and the expressed protein MfM4 shown to be responsible for loading acyl groups from CoA onto the squalestatin core as the final step of biosynthesis. MfM4 appears to have a broad substrate selectivity for its acyl CoA substrate, allowing the in vitro synthesis of novel squalestatins.
UR - http://www.scopus.com/inward/record.url?scp=84970005923&partnerID=8YFLogxK
U2 - 10.1039/c6cc02130a
DO - 10.1039/c6cc02130a
M3 - Article
C2 - 27056201
AN - SCOPUS:84970005923
VL - 52
SP - 6777
EP - 6780
JO - Chemical communications
JF - Chemical communications
SN - 1359-7345
IS - 41
ER -