Details
| Original language | English |
|---|---|
| Pages (from-to) | 589-598 |
| Number of pages | 10 |
| Journal | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| Volume | 43 |
| Issue number | 2 |
| Publication status | Published - 1 Jan 2015 |
Abstract
Adverse environmental conditions limit crop yield and better understanding of plant response to stress will assist the development of more tolerant cultivars. Maize and common bean plantlets were evaluated under salinity, high temperature, drought and waterlogged conditions to identify biochemical markers which could be useful for rapid identification of putative stress tolerant plants. The levels of phenolics (free, cell wall-linked, total), aldehydes including malondialdehyde and chlorophylls (a, b, total) were measured on stressed plantlets. Only two indicators were statistically non-significant: chlorophyll b in maize plantlets stressed with sodium chloride and malondialdehyde content in drought stressed maize. The most remarkable effects of abiotic stresses can be summarized as follows: (i) salinity increased levels of free phenolics in maize plantlets and chlorophylls (a, b, total) in common bean; (ii) high temperature (40 °C) elevated levels of chlorophylls (a, b, total) in maize but decreased chlorophylls (a, b, total) and free phenolics in common bean; (iii) drought increased phenolics and decreased chlorophylls (a, b, total) in maize and increased chlorophyll pigments (a, b, total) in common bean; (iv) waterlogging increased free phenolics and decreased chlorophylls (a, b, total) in maize and increased chlorophyll (a, total) in common bean. Free phenolics and chlorophylls, especially a, were the most responsive indicators to stress and can, therefore, be considered putative biochemical markers for abiotic stress tolerance in maize and common bean. The use of Fisher's linear discriminant analysis to differentiate non-stressed and stressed plants in breeding programs is also a novel aspect of this report. Fisher's linear discriminant functions classified correctly 100% of non-stressed or stressed originally grouped plants.
Keywords
- Abiotic stress, Biochemical markers, Genetic improvement, Phaseolus vulgaris, Zea mays
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Vol. 43, No. 2, 01.01.2015, p. 589-598.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Identification of discriminant factors after exposure of maize and common bean plantlets to abiotic stresses
AU - Hernández, Lázaro
AU - Loyola-González, Octavio
AU - Valle, Bárbara
AU - Martínez, Julia
AU - Díaz-López, Leyanes
AU - Aragón, Carlos
AU - Vicente, Oscar
AU - Papenbrock, Jutta
AU - Trethowan, Richard
AU - Yabor, Lourdes
AU - Lorenzo, José Carlos
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Adverse environmental conditions limit crop yield and better understanding of plant response to stress will assist the development of more tolerant cultivars. Maize and common bean plantlets were evaluated under salinity, high temperature, drought and waterlogged conditions to identify biochemical markers which could be useful for rapid identification of putative stress tolerant plants. The levels of phenolics (free, cell wall-linked, total), aldehydes including malondialdehyde and chlorophylls (a, b, total) were measured on stressed plantlets. Only two indicators were statistically non-significant: chlorophyll b in maize plantlets stressed with sodium chloride and malondialdehyde content in drought stressed maize. The most remarkable effects of abiotic stresses can be summarized as follows: (i) salinity increased levels of free phenolics in maize plantlets and chlorophylls (a, b, total) in common bean; (ii) high temperature (40 °C) elevated levels of chlorophylls (a, b, total) in maize but decreased chlorophylls (a, b, total) and free phenolics in common bean; (iii) drought increased phenolics and decreased chlorophylls (a, b, total) in maize and increased chlorophyll pigments (a, b, total) in common bean; (iv) waterlogging increased free phenolics and decreased chlorophylls (a, b, total) in maize and increased chlorophyll (a, total) in common bean. Free phenolics and chlorophylls, especially a, were the most responsive indicators to stress and can, therefore, be considered putative biochemical markers for abiotic stress tolerance in maize and common bean. The use of Fisher's linear discriminant analysis to differentiate non-stressed and stressed plants in breeding programs is also a novel aspect of this report. Fisher's linear discriminant functions classified correctly 100% of non-stressed or stressed originally grouped plants.
AB - Adverse environmental conditions limit crop yield and better understanding of plant response to stress will assist the development of more tolerant cultivars. Maize and common bean plantlets were evaluated under salinity, high temperature, drought and waterlogged conditions to identify biochemical markers which could be useful for rapid identification of putative stress tolerant plants. The levels of phenolics (free, cell wall-linked, total), aldehydes including malondialdehyde and chlorophylls (a, b, total) were measured on stressed plantlets. Only two indicators were statistically non-significant: chlorophyll b in maize plantlets stressed with sodium chloride and malondialdehyde content in drought stressed maize. The most remarkable effects of abiotic stresses can be summarized as follows: (i) salinity increased levels of free phenolics in maize plantlets and chlorophylls (a, b, total) in common bean; (ii) high temperature (40 °C) elevated levels of chlorophylls (a, b, total) in maize but decreased chlorophylls (a, b, total) and free phenolics in common bean; (iii) drought increased phenolics and decreased chlorophylls (a, b, total) in maize and increased chlorophyll pigments (a, b, total) in common bean; (iv) waterlogging increased free phenolics and decreased chlorophylls (a, b, total) in maize and increased chlorophyll (a, total) in common bean. Free phenolics and chlorophylls, especially a, were the most responsive indicators to stress and can, therefore, be considered putative biochemical markers for abiotic stress tolerance in maize and common bean. The use of Fisher's linear discriminant analysis to differentiate non-stressed and stressed plants in breeding programs is also a novel aspect of this report. Fisher's linear discriminant functions classified correctly 100% of non-stressed or stressed originally grouped plants.
KW - Abiotic stress
KW - Biochemical markers
KW - Genetic improvement
KW - Phaseolus vulgaris
KW - Zea mays
UR - http://www.scopus.com/inward/record.url?scp=84952321400&partnerID=8YFLogxK
U2 - 10.15835/nbha4329916
DO - 10.15835/nbha4329916
M3 - Article
AN - SCOPUS:84952321400
VL - 43
SP - 589
EP - 598
JO - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
SN - 0255-965X
IS - 2
ER -