Details
| Original language | English |
|---|---|
| Pages (from-to) | 245-9 |
| Number of pages | 5 |
| Journal | FEBS letters |
| Volume | 527 |
| Issue number | 1-3 |
| Publication status | Published - 11 Sept 2002 |
Abstract
The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.
Keywords
- Amino Acid Sequence, Archaeal Proteins/genetics, Bacterial Proteins, Biological Transport, Cloning, Molecular, Escherichia coli/genetics, Escherichia coli Proteins, Genetic Complementation Test, Hydrogen-Ion Concentration, Membrane Proteins/genetics, Methanococcus/genetics, Molecular Sequence Data, Multigene Family, Mutation, Phylogeny, Sequence Homology, Amino Acid, Sodium/metabolism, Sodium-Hydrogen Exchangers/genetics
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In: FEBS letters, Vol. 527, No. 1-3, 11.09.2002, p. 245-9.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii
AU - Hellmer, Jens
AU - Pätzold, Ralf
AU - Zeilinger, Carsten
PY - 2002/9/11
Y1 - 2002/9/11
N2 - The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.
AB - The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.
KW - Amino Acid Sequence
KW - Archaeal Proteins/genetics
KW - Bacterial Proteins
KW - Biological Transport
KW - Cloning, Molecular
KW - Escherichia coli/genetics
KW - Escherichia coli Proteins
KW - Genetic Complementation Test
KW - Hydrogen-Ion Concentration
KW - Membrane Proteins/genetics
KW - Methanococcus/genetics
KW - Molecular Sequence Data
KW - Multigene Family
KW - Mutation
KW - Phylogeny
KW - Sequence Homology, Amino Acid
KW - Sodium/metabolism
KW - Sodium-Hydrogen Exchangers/genetics
U2 - 10.1016/s0014-5793(02)03244-1
DO - 10.1016/s0014-5793(02)03244-1
M3 - Article
C2 - 12220668
VL - 527
SP - 245
EP - 249
JO - FEBS letters
JF - FEBS letters
SN - 0014-5793
IS - 1-3
ER -