Details
| Original language | English |
|---|---|
| Pages (from-to) | 766-776 |
| Number of pages | 11 |
| Journal | Journal of plant physiology |
| Volume | 165 |
| Issue number | 7 |
| Publication status | Published - 5 May 2008 |
| Externally published | Yes |
Abstract
Branched 1,6-1,3-β-glucans from Phytophthora sojae cell walls represent pathogen-associated molecular patterns (PAMPs) that have been shown to mediate the activation of plant defence reactions in many legumes. In soybean, a receptor protein complex containing a high affinity β-glucan-binding protein (GBP) was identified and investigated in detail. In the model legume Medicago truncatula, used for functional genomic studies of various plant-microbe interactions, a high-affinity β-glucan-binding site was characterized biochemically. However, to date, none of the genes encoding GBPs from M. truncatula have been described. Here, we report the identification of four full-length clones encoding putative β-glucan-binding proteins from M. truncatula, MtGBP1, 2, 3, and 4, composing a multigene family encoding GBP-related proteins in this plant. Differences in expression patterns as well as in regulation on treatment with two different biotic elicitors are demonstrated for the members of the GBP family and for a selection of defence-related genes.
Keywords
- Elicitors, Medicago truncatula, Microarray, Plant defence, β-Glucan receptor
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of plant physiology, Vol. 165, No. 7, 05.05.2008, p. 766-776.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Identification of a multigene family encoding putative β-glucan-binding proteins in Medicago truncatula
AU - Leclercq, Julie
AU - Fliegmann, Judith
AU - Tellström, Verena
AU - Niebel, Andreas
AU - Cullimore, Julie V.
AU - Niehaus, Karsten
AU - Küster, Helge
AU - Ebel, Jürgen
AU - Mithöfer, Axel
N1 - Funding information: We thank K. Schmieja for excellent technical assistance. We are grateful to M.V. Silber for valuable discussions. This work was supported by the European Community's Human Potential Programme under contract HPRN-CT-2002-00251, SACC-SIG-NET, the Deutsche Forschungsgemeinschaft “DFG-Initiative Bioinformatik und Genomforschung”, and the International NRW Graduate School in Bioinformatics and Genome Research. J.L. benefited from a fellowship on the EC SACC-SIG-NET contract.
PY - 2008/5/5
Y1 - 2008/5/5
N2 - Branched 1,6-1,3-β-glucans from Phytophthora sojae cell walls represent pathogen-associated molecular patterns (PAMPs) that have been shown to mediate the activation of plant defence reactions in many legumes. In soybean, a receptor protein complex containing a high affinity β-glucan-binding protein (GBP) was identified and investigated in detail. In the model legume Medicago truncatula, used for functional genomic studies of various plant-microbe interactions, a high-affinity β-glucan-binding site was characterized biochemically. However, to date, none of the genes encoding GBPs from M. truncatula have been described. Here, we report the identification of four full-length clones encoding putative β-glucan-binding proteins from M. truncatula, MtGBP1, 2, 3, and 4, composing a multigene family encoding GBP-related proteins in this plant. Differences in expression patterns as well as in regulation on treatment with two different biotic elicitors are demonstrated for the members of the GBP family and for a selection of defence-related genes.
AB - Branched 1,6-1,3-β-glucans from Phytophthora sojae cell walls represent pathogen-associated molecular patterns (PAMPs) that have been shown to mediate the activation of plant defence reactions in many legumes. In soybean, a receptor protein complex containing a high affinity β-glucan-binding protein (GBP) was identified and investigated in detail. In the model legume Medicago truncatula, used for functional genomic studies of various plant-microbe interactions, a high-affinity β-glucan-binding site was characterized biochemically. However, to date, none of the genes encoding GBPs from M. truncatula have been described. Here, we report the identification of four full-length clones encoding putative β-glucan-binding proteins from M. truncatula, MtGBP1, 2, 3, and 4, composing a multigene family encoding GBP-related proteins in this plant. Differences in expression patterns as well as in regulation on treatment with two different biotic elicitors are demonstrated for the members of the GBP family and for a selection of defence-related genes.
KW - Elicitors
KW - Medicago truncatula
KW - Microarray
KW - Plant defence
KW - β-Glucan receptor
UR - http://www.scopus.com/inward/record.url?scp=41549147219&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2007.02.008
DO - 10.1016/j.jplph.2007.02.008
M3 - Article
C2 - 17728012
AN - SCOPUS:41549147219
VL - 165
SP - 766
EP - 776
JO - Journal of plant physiology
JF - Journal of plant physiology
SN - 0176-1617
IS - 7
ER -