Details
| Original language | English |
|---|---|
| Pages (from-to) | 233-244 |
| Number of pages | 12 |
| Journal | Journal of natural products |
| Volume | 88 |
| Issue number | 2 |
| Early online date | 23 Jan 2025 |
| Publication status | Published - 28 Feb 2025 |
Abstract
Xylindein is a blue-green pigment produced by the fungi Chlorociboria aeruginascens and Chlorociboria aeruginosa. Its stunning color and optoelectronic properties make xylindein valuable for textiles and as a natural semiconductor material. However, producing xylindein from culture broths remains challenging because of the slow growth of the Chlorociboria species and the poor solubility of xylindein in organic solvents. An alternative production route for obtaining pure xylindein is heterologous expression of the xylindein biosynthetic genes. Here, we resequenced the genome of C. aeruginascens and C. aeruginosa, and subsequent genome mining and phylogenetic dereplication identified a unique candidate biosynthetic gene cluster with a nonreducing polyketide synthase (nrPKS). RNA sequencing during xylindein production revealed that the core gene XLNpks is co-regulated with eight other genes at the locus. Among those, XLNfas1 and XLNfas2 encode a putative fatty acid synthase, which likely provides the starter unit to XLNpks. Attempts to heterologously express in Aspergillus oryzae XLNpks alone or in combination with XLNfas1 and XLNfas2 did not yield any intermediate, but expression of the closely related viriditoxin nrPKS (VdtA) produced the expected intermediate. Based on our results, we propose a biosynthetic route to xylindein and suggest that the obtained A. oryzae transformants open ways to further study xylindein biosynthesis.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmacology
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
- Pharmacology, Toxicology and Pharmaceutics(all)
- Drug Discovery
- Medicine(all)
- Complementary and alternative medicine
- Chemistry(all)
- Organic Chemistry
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In: Journal of natural products, Vol. 88, No. 2, 28.02.2025, p. 233-244.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Identification of a Biosynthetic Gene Cluster for the Production of the Blue-Green Pigment Xylindein by the Fungus Chlorociboria aeruginascens
AU - Guo, Yanfang
AU - Navarro-Muñoz, Jorge
AU - Rodenbach, Caroline
AU - Dwars, Elske
AU - Dieleman, Chendo
AU - van den Hout, Bart
AU - Sanders, Bazante
AU - Zhou, Miaomiao
AU - Arogunjo, Ayodele
AU - Cox, Russell J.
AU - Driessen, Arnold J.M.
AU - Collemare, Jérôme
PY - 2025/2/28
Y1 - 2025/2/28
N2 - Xylindein is a blue-green pigment produced by the fungi Chlorociboria aeruginascens and Chlorociboria aeruginosa. Its stunning color and optoelectronic properties make xylindein valuable for textiles and as a natural semiconductor material. However, producing xylindein from culture broths remains challenging because of the slow growth of the Chlorociboria species and the poor solubility of xylindein in organic solvents. An alternative production route for obtaining pure xylindein is heterologous expression of the xylindein biosynthetic genes. Here, we resequenced the genome of C. aeruginascens and C. aeruginosa, and subsequent genome mining and phylogenetic dereplication identified a unique candidate biosynthetic gene cluster with a nonreducing polyketide synthase (nrPKS). RNA sequencing during xylindein production revealed that the core gene XLNpks is co-regulated with eight other genes at the locus. Among those, XLNfas1 and XLNfas2 encode a putative fatty acid synthase, which likely provides the starter unit to XLNpks. Attempts to heterologously express in Aspergillus oryzae XLNpks alone or in combination with XLNfas1 and XLNfas2 did not yield any intermediate, but expression of the closely related viriditoxin nrPKS (VdtA) produced the expected intermediate. Based on our results, we propose a biosynthetic route to xylindein and suggest that the obtained A. oryzae transformants open ways to further study xylindein biosynthesis.
AB - Xylindein is a blue-green pigment produced by the fungi Chlorociboria aeruginascens and Chlorociboria aeruginosa. Its stunning color and optoelectronic properties make xylindein valuable for textiles and as a natural semiconductor material. However, producing xylindein from culture broths remains challenging because of the slow growth of the Chlorociboria species and the poor solubility of xylindein in organic solvents. An alternative production route for obtaining pure xylindein is heterologous expression of the xylindein biosynthetic genes. Here, we resequenced the genome of C. aeruginascens and C. aeruginosa, and subsequent genome mining and phylogenetic dereplication identified a unique candidate biosynthetic gene cluster with a nonreducing polyketide synthase (nrPKS). RNA sequencing during xylindein production revealed that the core gene XLNpks is co-regulated with eight other genes at the locus. Among those, XLNfas1 and XLNfas2 encode a putative fatty acid synthase, which likely provides the starter unit to XLNpks. Attempts to heterologously express in Aspergillus oryzae XLNpks alone or in combination with XLNfas1 and XLNfas2 did not yield any intermediate, but expression of the closely related viriditoxin nrPKS (VdtA) produced the expected intermediate. Based on our results, we propose a biosynthetic route to xylindein and suggest that the obtained A. oryzae transformants open ways to further study xylindein biosynthesis.
UR - http://www.scopus.com/inward/record.url?scp=86000674252&partnerID=8YFLogxK
U2 - 10.1021/acs.jnatprod.4c00350
DO - 10.1021/acs.jnatprod.4c00350
M3 - Article
C2 - 39847046
AN - SCOPUS:86000674252
VL - 88
SP - 233
EP - 244
JO - Journal of natural products
JF - Journal of natural products
SN - 0163-3864
IS - 2
ER -