Details
| Original language | English |
|---|---|
| Pages (from-to) | 147-158 |
| Number of pages | 11 |
| Journal | Malaysian Applied Biology |
| Volume | 53 |
| Issue number | 3 |
| Publication status | Published - 30 Sept 2024 |
Abstract
Keywords
- Botrytis cinerea, gray mold, Lycopersicon esculentum Mill, pathogenicity, tomato
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- General Agricultural and Biological Sciences
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In: Malaysian Applied Biology, Vol. 53, No. 3, 30.09.2024, p. 147-158.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Identification and Characterization of Botrytis cinerea Causing Gray Mold on Tomatoes in Cameron Highlands, Malaysia
AU - Yusoff, Siti Fairuz
AU - Ismail, Siti Izera
AU - Farhanah Haron, Farah
AU - Safari, Zahir Shah
AU - Hashim, Rohasmizah
PY - 2024/9/30
Y1 - 2024/9/30
N2 - Botrytis cinerea, commonly known as gray mold, is a pervasive fungal pathogen that affects a wide range of plant species, leading to significant agricultural losses. The identification of Botrytis cinerea in Malaysia is crucial for protecting the agricultural sector, minimizing economic losses, ensuring food security, maintaining export quality, addressing environmental concerns, and advancing scientific research. In the present research, tomato fruits collected from Cameron Highlands, Pahang, Malaysia showed gray mold disease symptoms of B. cinerea. The fungal isolates were examined morphologically for colony colour, growth rate, conidiophores, conidia shape, and sclerotia on PDA and V8 agar. According to the results, conidiophores appeared in grape shape and length was range of 21.26-32.52 μm, ovoid conidial dimensions were in the range of 10.03-16.08 × 7.37-11.15 μm and sclerotia size was range 1.91-4.50 × 1.70-4.00 mm. All isolates were attributed to the morphospecies Botrytis cinerea on account of these characteristics. The resulting sequences deposited in GenBank were accessions MT012053 to MT012062, respectively. A BLAST analysis of the resulting 550-bp nucleotide sequences showed 99-100% identity closest matched to B. cinerea. The pathogenicity experiments showed P6 isolates of B. cinerea were highly pathogenic and caused gray mold development on tomato fruits that led to severe symptoms in five days. Meanwhile, the least pathogenic isolate was P9. In terms of temperature, B. cinerea grew faster on PDA at 20ºC, slower grew below 20ºC and did not grow at 25ºC. Identification and characterization of B. cinerea on tomato could potentially provide information to assist disease management strategies for B. cinerea.
AB - Botrytis cinerea, commonly known as gray mold, is a pervasive fungal pathogen that affects a wide range of plant species, leading to significant agricultural losses. The identification of Botrytis cinerea in Malaysia is crucial for protecting the agricultural sector, minimizing economic losses, ensuring food security, maintaining export quality, addressing environmental concerns, and advancing scientific research. In the present research, tomato fruits collected from Cameron Highlands, Pahang, Malaysia showed gray mold disease symptoms of B. cinerea. The fungal isolates were examined morphologically for colony colour, growth rate, conidiophores, conidia shape, and sclerotia on PDA and V8 agar. According to the results, conidiophores appeared in grape shape and length was range of 21.26-32.52 μm, ovoid conidial dimensions were in the range of 10.03-16.08 × 7.37-11.15 μm and sclerotia size was range 1.91-4.50 × 1.70-4.00 mm. All isolates were attributed to the morphospecies Botrytis cinerea on account of these characteristics. The resulting sequences deposited in GenBank were accessions MT012053 to MT012062, respectively. A BLAST analysis of the resulting 550-bp nucleotide sequences showed 99-100% identity closest matched to B. cinerea. The pathogenicity experiments showed P6 isolates of B. cinerea were highly pathogenic and caused gray mold development on tomato fruits that led to severe symptoms in five days. Meanwhile, the least pathogenic isolate was P9. In terms of temperature, B. cinerea grew faster on PDA at 20ºC, slower grew below 20ºC and did not grow at 25ºC. Identification and characterization of B. cinerea on tomato could potentially provide information to assist disease management strategies for B. cinerea.
KW - Botrytis cinerea
KW - gray mold
KW - Lycopersicon esculentum Mill
KW - pathogenicity
KW - tomato
UR - http://www.scopus.com/inward/record.url?scp=85206388242&partnerID=8YFLogxK
U2 - 10.55230/mabjournal.v53i3.2880
DO - 10.55230/mabjournal.v53i3.2880
M3 - Article
VL - 53
SP - 147
EP - 158
JO - Malaysian Applied Biology
JF - Malaysian Applied Biology
SN - 2462-151X
IS - 3
ER -