Details
| Original language | English |
|---|---|
| Pages (from-to) | e552-e565 |
| Journal | Artificial Organs |
| Volume | 44 |
| Issue number | 12 |
| Early online date | 15 Jul 2020 |
| Publication status | Published - 17 Dec 2020 |
Abstract
Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.
Keywords
- bioartificial lung, biohybrid lung, biohybrid organs, endothelialization, hypothermic preservation
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
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In: Artificial Organs, Vol. 44, No. 12, 17.12.2020, p. e552-e565.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Hypothermic preservation of endothelialized gas-exchange membranes
AU - Pflaum, Michael
AU - Merhej, Hayan
AU - Peredo, Ariana
AU - De, Adim
AU - Dipresa, Daniele
AU - Wiegmann, Bettina
AU - Wolkers, Willem
AU - Haverich, Axel
AU - Korossis, Sotirios
N1 - Funding Information: This study was supported in part by the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy EXC62: 24102914), the German Centre for Lung Research (DZL) BREATH (Biomedical Research In Endstage And Obstructive Lung Disease Hannover; DZL: 82DZL00201), the German Research Foundation (DFG; Projects WI 4088/1‐2, SPP2014: 347346497 and SPP2014: 348028075).
PY - 2020/12/17
Y1 - 2020/12/17
N2 - Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.
AB - Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.
KW - bioartificial lung
KW - biohybrid lung
KW - biohybrid organs
KW - endothelialization
KW - hypothermic preservation
UR - http://www.scopus.com/inward/record.url?scp=85089391652&partnerID=8YFLogxK
U2 - 10.1111/aor.13776
DO - 10.1111/aor.13776
M3 - Article
C2 - 32666514
AN - SCOPUS:85089391652
VL - 44
SP - e552-e565
JO - Artificial Organs
JF - Artificial Organs
SN - 0160-564X
IS - 12
ER -