Details
| Original language | English |
|---|---|
| Title of host publication | Optogenetics and Optical Manipulation 2018 |
| Publisher | SPIE |
| ISBN (electronic) | 9781510614499 |
| Publication status | Published - 14 Feb 2018 |
| Event | Optogenetics and Optical Manipulation 2018 - San Francisco, United States Duration: 27 Jan 2018 → 28 Jan 2018 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 10482 |
| ISSN (Print) | 1605-7422 |
Abstract
Light-based therapies have been established for various indications, such as skin conditions, cancer or neonatal jaundice. Advances in the field of optogenetics open up new horizons for light-tissue interactions with an organism-wide impact. Excitable tissues, such as nerve and muscle tissues, can be controlled by light after the introduction of light-sensitive ion channels. Since these organs are generally not easily accessible to illumination in vivo, there is an increasing need for effective biocompatible waveguides for light delivery. These devices not only have to guide and distribute the light as desired with minimal losses, they should also mimic the mechanical properties of the surrounding tissue to ensure compatibility. In this project, we are tuning the properties of hydrogels from poly(ethylene glycol) derivatives to achieve compatibility with muscle tissue as well as optimal light guiding and distribution for optogenetic applications at the heart. The excitation light is coupled into the hydrogel with a biocompatible fiber. Properties of the hydrogel are mainly tuned by monomer length and concentration. Total reflection can be achieved by embedding a fiber-like hydrogel with a high refractive index into a second, low refractive index gel. Different geometries and scattering microparticles are used for light distribution in a flat gel patch. Targeted cell attachment can be achieved by introducing a protein layer to the otherwise bioinert gel. After optimization, the hydrogel may be used to deliver light for the excitation of genetically altered cardiomyocytes for controlled contraction.
Keywords
- Biohybrid implant, Cardiac pacing, Defibrillation, Hydrogels, Light delivery, Optogenetics, Waveguiding
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Sustainable Development Goals
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Optogenetics and Optical Manipulation 2018. SPIE, 2018. 104820Q (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10482).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Hydrogels for efficient light delivery in optogenetic applications
AU - Johannsmeier, Sonja
AU - Torres, M. L.
AU - Ripken, T.
AU - Heinemann, D.
AU - Heisterkamp, A.
N1 - Funding information: This work is funded by the Federal Ministry of Education and Research, Germany, Grant no. 13N14085 and supported by the BIOFABRICATION FOR NIFE Initiative (VWZN2860). Figure 4a was kindly provided by Olga Simon.
PY - 2018/2/14
Y1 - 2018/2/14
N2 - Light-based therapies have been established for various indications, such as skin conditions, cancer or neonatal jaundice. Advances in the field of optogenetics open up new horizons for light-tissue interactions with an organism-wide impact. Excitable tissues, such as nerve and muscle tissues, can be controlled by light after the introduction of light-sensitive ion channels. Since these organs are generally not easily accessible to illumination in vivo, there is an increasing need for effective biocompatible waveguides for light delivery. These devices not only have to guide and distribute the light as desired with minimal losses, they should also mimic the mechanical properties of the surrounding tissue to ensure compatibility. In this project, we are tuning the properties of hydrogels from poly(ethylene glycol) derivatives to achieve compatibility with muscle tissue as well as optimal light guiding and distribution for optogenetic applications at the heart. The excitation light is coupled into the hydrogel with a biocompatible fiber. Properties of the hydrogel are mainly tuned by monomer length and concentration. Total reflection can be achieved by embedding a fiber-like hydrogel with a high refractive index into a second, low refractive index gel. Different geometries and scattering microparticles are used for light distribution in a flat gel patch. Targeted cell attachment can be achieved by introducing a protein layer to the otherwise bioinert gel. After optimization, the hydrogel may be used to deliver light for the excitation of genetically altered cardiomyocytes for controlled contraction.
AB - Light-based therapies have been established for various indications, such as skin conditions, cancer or neonatal jaundice. Advances in the field of optogenetics open up new horizons for light-tissue interactions with an organism-wide impact. Excitable tissues, such as nerve and muscle tissues, can be controlled by light after the introduction of light-sensitive ion channels. Since these organs are generally not easily accessible to illumination in vivo, there is an increasing need for effective biocompatible waveguides for light delivery. These devices not only have to guide and distribute the light as desired with minimal losses, they should also mimic the mechanical properties of the surrounding tissue to ensure compatibility. In this project, we are tuning the properties of hydrogels from poly(ethylene glycol) derivatives to achieve compatibility with muscle tissue as well as optimal light guiding and distribution for optogenetic applications at the heart. The excitation light is coupled into the hydrogel with a biocompatible fiber. Properties of the hydrogel are mainly tuned by monomer length and concentration. Total reflection can be achieved by embedding a fiber-like hydrogel with a high refractive index into a second, low refractive index gel. Different geometries and scattering microparticles are used for light distribution in a flat gel patch. Targeted cell attachment can be achieved by introducing a protein layer to the otherwise bioinert gel. After optimization, the hydrogel may be used to deliver light for the excitation of genetically altered cardiomyocytes for controlled contraction.
KW - Biohybrid implant
KW - Cardiac pacing
KW - Defibrillation
KW - Hydrogels
KW - Light delivery
KW - Optogenetics
KW - Waveguiding
UR - http://www.scopus.com/inward/record.url?scp=85046997530&partnerID=8YFLogxK
U2 - 10.1117/12.2289470
DO - 10.1117/12.2289470
M3 - Conference contribution
AN - SCOPUS:85046997530
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optogenetics and Optical Manipulation 2018
PB - SPIE
T2 - Optogenetics and Optical Manipulation 2018
Y2 - 27 January 2018 through 28 January 2018
ER -