Details
| Original language | English |
|---|---|
| Pages (from-to) | 623-636 |
| Number of pages | 14 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 99 |
| Issue number | 2 |
| Publication status | Published - Jan 2015 |
Abstract
Hydrogels have become one of the most popular platforms for three-dimensional (3D) cultivation of mammalian cells. The enormous versatility of hydrogel materials makes it possible to design scaffolds with predefined mechanical properties, as well as with desired biofunctionality. 3D hydrogel constructs have been used for a variety of applications, including tissue engineering of microorgan systems, drug delivery, cytotoxicity testing, and drug screening. Moreover, 3D culture is applied for investigating cellular physiology, stem cell differentiation, and tumor models and for studying interaction mechanisms between the extracellular matrix and cells. In this paper, we review current examples of performance-based hydrogel design for 3D cell culture applications. A major emphasis is placed on a description of how standard analytical protocols and imaging techniques are being adapted to analysis of 3D cell culture in hydrogel systems.
Keywords
- 3D analytics, 3D cell culture, Hydrogel, Mammalian cell culture, Material design
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 99, No. 2, 01.2015, p. 623-636.
Research output: Contribution to journal › Article › Research
}
TY - JOUR
T1 - Hydrogels for 3D mammalian cell culture: a starting guide for laboratory practice
AU - Ruedinger, Ferdinand
AU - Lavrentieva, Antonina
AU - Blume, Cornelia
AU - Pepelanova, Iliyana
AU - Scheper, Thomas
N1 - Funding information: This work was performed in the framework BIOFABRICATION FOR NIFE funded by the state of Lower Saxony, Germany.
PY - 2015/1
Y1 - 2015/1
N2 - Hydrogels have become one of the most popular platforms for three-dimensional (3D) cultivation of mammalian cells. The enormous versatility of hydrogel materials makes it possible to design scaffolds with predefined mechanical properties, as well as with desired biofunctionality. 3D hydrogel constructs have been used for a variety of applications, including tissue engineering of microorgan systems, drug delivery, cytotoxicity testing, and drug screening. Moreover, 3D culture is applied for investigating cellular physiology, stem cell differentiation, and tumor models and for studying interaction mechanisms between the extracellular matrix and cells. In this paper, we review current examples of performance-based hydrogel design for 3D cell culture applications. A major emphasis is placed on a description of how standard analytical protocols and imaging techniques are being adapted to analysis of 3D cell culture in hydrogel systems.
AB - Hydrogels have become one of the most popular platforms for three-dimensional (3D) cultivation of mammalian cells. The enormous versatility of hydrogel materials makes it possible to design scaffolds with predefined mechanical properties, as well as with desired biofunctionality. 3D hydrogel constructs have been used for a variety of applications, including tissue engineering of microorgan systems, drug delivery, cytotoxicity testing, and drug screening. Moreover, 3D culture is applied for investigating cellular physiology, stem cell differentiation, and tumor models and for studying interaction mechanisms between the extracellular matrix and cells. In this paper, we review current examples of performance-based hydrogel design for 3D cell culture applications. A major emphasis is placed on a description of how standard analytical protocols and imaging techniques are being adapted to analysis of 3D cell culture in hydrogel systems.
KW - 3D analytics
KW - 3D cell culture
KW - Hydrogel
KW - Mammalian cell culture
KW - Material design
UR - http://www.scopus.com/inward/record.url?scp=84925511237&partnerID=8YFLogxK
U2 - 10.1007/s00253-014-6253-y
DO - 10.1007/s00253-014-6253-y
M3 - Article
C2 - 25432676
AN - SCOPUS:84925511237
VL - 99
SP - 623
EP - 636
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 2
ER -