Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L.

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Edit Lantos
  • Reiner Krämer
  • Katja R. Richert-Pöggeler
  • Edgar Maiss
  • Janine König
  • Thomas Nothnagel

External Research Organisations

  • Julius Kühn Institute - Federal Research Centre for Cultivated Plants (JKI)
View graph of relations

Details

Original languageEnglish
Article number1187563
JournalFrontiers in Plant Science
Volume14
Publication statusPublished - 31 Jul 2023

Abstract

Asparagus samples were examined from growing areas of Germany and selected European as well as North, Central and South American countries. Overall, 474 samples were analyzed for Asparagus virus 1 (AV1) using DAS-ELISA. In our survey, 19 AV1 isolates were further characterized. Experimental transmission to 11 species belonging to Aizoaceae, Amarantaceae, Asparagaceae, and Solanaceae succeeded. The ultrastructure of AV1 infection in asparagus has been revealed and has been compared with the one in indicator plants. The cylindrical inclusion (CI) protein, a core factor in viral replication, localized within the cytoplasm and in systemic infections adjacent to the plasmodesmata. The majority of isolates referred to pathotype I (PI). These triggered a hypersensitive resistance in inoculated leaves of Chenopodium spp. and were incapable of infecting Nicotiana spp. Only pathotype II (PII) and pathotype III (PIII) infected Nicotiana benthamiana systemically but differed in their virulence when transmitted to Chenopodium spp. The newly identified PIII generated amorphous inclusion bodies and degraded chloroplasts during systemic infection but not in local lesions of infected Chenopodium spp. PIII probably evolved via recombination in asparagus carrying a mixed infection by PI and PII. Phylogeny of the coat protein region recognized two clusters, which did not overlap with the CI-associated grouping of pathotypes. These results provide evidence for ongoing modular evolution of AV1.

Keywords

    Asparagus officinalis, Asparagus virus 1 (AV1), cylindrical inclusions, host range, pathotype, phylogeny of coat protein region, ultrastructural changes, virus incidence

ASJC Scopus subject areas

Cite this

Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L. / Lantos, Edit; Krämer, Reiner; Richert-Pöggeler, Katja R. et al.
In: Frontiers in Plant Science, Vol. 14, 1187563, 31.07.2023.

Research output: Contribution to journalArticleResearchpeer review

Lantos E, Krämer R, Richert-Pöggeler KR, Maiss E, König J, Nothnagel T. Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L. Frontiers in Plant Science. 2023 Jul 31;14:1187563. doi: 10.3389/fpls.2023.1187563, 10.15488/15385
Download
@article{76d9e2135a2a47bea6c2de2695399be4,
title = "Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L.",
abstract = "Asparagus samples were examined from growing areas of Germany and selected European as well as North, Central and South American countries. Overall, 474 samples were analyzed for Asparagus virus 1 (AV1) using DAS-ELISA. In our survey, 19 AV1 isolates were further characterized. Experimental transmission to 11 species belonging to Aizoaceae, Amarantaceae, Asparagaceae, and Solanaceae succeeded. The ultrastructure of AV1 infection in asparagus has been revealed and has been compared with the one in indicator plants. The cylindrical inclusion (CI) protein, a core factor in viral replication, localized within the cytoplasm and in systemic infections adjacent to the plasmodesmata. The majority of isolates referred to pathotype I (PI). These triggered a hypersensitive resistance in inoculated leaves of Chenopodium spp. and were incapable of infecting Nicotiana spp. Only pathotype II (PII) and pathotype III (PIII) infected Nicotiana benthamiana systemically but differed in their virulence when transmitted to Chenopodium spp. The newly identified PIII generated amorphous inclusion bodies and degraded chloroplasts during systemic infection but not in local lesions of infected Chenopodium spp. PIII probably evolved via recombination in asparagus carrying a mixed infection by PI and PII. Phylogeny of the coat protein region recognized two clusters, which did not overlap with the CI-associated grouping of pathotypes. These results provide evidence for ongoing modular evolution of AV1.",
keywords = "Asparagus officinalis, Asparagus virus 1 (AV1), cylindrical inclusions, host range, pathotype, phylogeny of coat protein region, ultrastructural changes, virus incidence",
author = "Edit Lantos and Reiner Kr{\"a}mer and Richert-P{\"o}ggeler, {Katja R.} and Edgar Maiss and Janine K{\"o}nig and Thomas Nothnagel",
note = "Funding Information: This work was partly funded by the Federal Ministry of Food and Agriculture (BMEL-B{\"O}LN 312-06.01-2812NA078).",
year = "2023",
month = jul,
day = "31",
doi = "10.3389/fpls.2023.1187563",
language = "English",
volume = "14",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A.",

}

Download

TY - JOUR

T1 - Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L.

AU - Lantos, Edit

AU - Krämer, Reiner

AU - Richert-Pöggeler, Katja R.

AU - Maiss, Edgar

AU - König, Janine

AU - Nothnagel, Thomas

N1 - Funding Information: This work was partly funded by the Federal Ministry of Food and Agriculture (BMEL-BÖLN 312-06.01-2812NA078).

PY - 2023/7/31

Y1 - 2023/7/31

N2 - Asparagus samples were examined from growing areas of Germany and selected European as well as North, Central and South American countries. Overall, 474 samples were analyzed for Asparagus virus 1 (AV1) using DAS-ELISA. In our survey, 19 AV1 isolates were further characterized. Experimental transmission to 11 species belonging to Aizoaceae, Amarantaceae, Asparagaceae, and Solanaceae succeeded. The ultrastructure of AV1 infection in asparagus has been revealed and has been compared with the one in indicator plants. The cylindrical inclusion (CI) protein, a core factor in viral replication, localized within the cytoplasm and in systemic infections adjacent to the plasmodesmata. The majority of isolates referred to pathotype I (PI). These triggered a hypersensitive resistance in inoculated leaves of Chenopodium spp. and were incapable of infecting Nicotiana spp. Only pathotype II (PII) and pathotype III (PIII) infected Nicotiana benthamiana systemically but differed in their virulence when transmitted to Chenopodium spp. The newly identified PIII generated amorphous inclusion bodies and degraded chloroplasts during systemic infection but not in local lesions of infected Chenopodium spp. PIII probably evolved via recombination in asparagus carrying a mixed infection by PI and PII. Phylogeny of the coat protein region recognized two clusters, which did not overlap with the CI-associated grouping of pathotypes. These results provide evidence for ongoing modular evolution of AV1.

AB - Asparagus samples were examined from growing areas of Germany and selected European as well as North, Central and South American countries. Overall, 474 samples were analyzed for Asparagus virus 1 (AV1) using DAS-ELISA. In our survey, 19 AV1 isolates were further characterized. Experimental transmission to 11 species belonging to Aizoaceae, Amarantaceae, Asparagaceae, and Solanaceae succeeded. The ultrastructure of AV1 infection in asparagus has been revealed and has been compared with the one in indicator plants. The cylindrical inclusion (CI) protein, a core factor in viral replication, localized within the cytoplasm and in systemic infections adjacent to the plasmodesmata. The majority of isolates referred to pathotype I (PI). These triggered a hypersensitive resistance in inoculated leaves of Chenopodium spp. and were incapable of infecting Nicotiana spp. Only pathotype II (PII) and pathotype III (PIII) infected Nicotiana benthamiana systemically but differed in their virulence when transmitted to Chenopodium spp. The newly identified PIII generated amorphous inclusion bodies and degraded chloroplasts during systemic infection but not in local lesions of infected Chenopodium spp. PIII probably evolved via recombination in asparagus carrying a mixed infection by PI and PII. Phylogeny of the coat protein region recognized two clusters, which did not overlap with the CI-associated grouping of pathotypes. These results provide evidence for ongoing modular evolution of AV1.

KW - Asparagus officinalis

KW - Asparagus virus 1 (AV1)

KW - cylindrical inclusions

KW - host range

KW - pathotype

KW - phylogeny of coat protein region

KW - ultrastructural changes

KW - virus incidence

UR - http://www.scopus.com/inward/record.url?scp=85168150542&partnerID=8YFLogxK

U2 - 10.3389/fpls.2023.1187563

DO - 10.3389/fpls.2023.1187563

M3 - Article

AN - SCOPUS:85168150542

VL - 14

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1187563

ER -