Details
| Original language | English |
|---|---|
| Pages (from-to) | 5419-5430 |
| Number of pages | 12 |
| Journal | Optics Express |
| Volume | 19 |
| Issue number | 6 |
| Publication status | Published - 14 Mar 2011 |
Abstract
Optical Projection Tomography (OPT) proved to be useful for the three-dimensional tracking of fluorescence signals in biological model organisms with sizes up to several millimeters. This tomographic technique detects absorption as well as fluorescence to create multimodal three-dimensional data. While the absorption of a specimen is detected very fast usually less than 0.1% of the fluorescence photons are collected. The low efficiency can result in radiation dose dependent artifacts such as photobleaching and phototoxicity. To minimize these effects as well as artifacts introduced due to the use of a CCD-or CMOS-camera-chip, we constructed a Scanning Laser Optical Tomograph (SLOT). Compared to conventional fluorescence OPT our first SLOT enhanced the photon collection efficiency a hundredfold.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
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In: Optics Express, Vol. 19, No. 6, 14.03.2011, p. 5419-5430.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Highly efficient 3D fluorescence microscopy with a Scanning Laser Optical Tomograph
AU - Lorbeer, Raoul Amadeus
AU - Heidrich, Marko
AU - Lorbeer, Christina
AU - Ojeda, Diego Fernando Ramírez
AU - Bicker, Gerd
AU - Meyer, Heiko
AU - Heisterkamp, Alexander
PY - 2011/3/14
Y1 - 2011/3/14
N2 - Optical Projection Tomography (OPT) proved to be useful for the three-dimensional tracking of fluorescence signals in biological model organisms with sizes up to several millimeters. This tomographic technique detects absorption as well as fluorescence to create multimodal three-dimensional data. While the absorption of a specimen is detected very fast usually less than 0.1% of the fluorescence photons are collected. The low efficiency can result in radiation dose dependent artifacts such as photobleaching and phototoxicity. To minimize these effects as well as artifacts introduced due to the use of a CCD-or CMOS-camera-chip, we constructed a Scanning Laser Optical Tomograph (SLOT). Compared to conventional fluorescence OPT our first SLOT enhanced the photon collection efficiency a hundredfold.
AB - Optical Projection Tomography (OPT) proved to be useful for the three-dimensional tracking of fluorescence signals in biological model organisms with sizes up to several millimeters. This tomographic technique detects absorption as well as fluorescence to create multimodal three-dimensional data. While the absorption of a specimen is detected very fast usually less than 0.1% of the fluorescence photons are collected. The low efficiency can result in radiation dose dependent artifacts such as photobleaching and phototoxicity. To minimize these effects as well as artifacts introduced due to the use of a CCD-or CMOS-camera-chip, we constructed a Scanning Laser Optical Tomograph (SLOT). Compared to conventional fluorescence OPT our first SLOT enhanced the photon collection efficiency a hundredfold.
UR - http://www.scopus.com/inward/record.url?scp=79952590834&partnerID=8YFLogxK
U2 - 10.1364/OE.19.005419
DO - 10.1364/OE.19.005419
M3 - Article
C2 - 21445181
AN - SCOPUS:79952590834
VL - 19
SP - 5419
EP - 5430
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 6
ER -