Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea

Research output: Contribution to journal › Article › Research › peer review

Authors

  • B. S. Shavkuta
  • M. Y. Gerasimov
  • N. V. Minaev
  • D. S. Kuznetsova
  • V. V. Dudenkova
  • I. A. Mushkova
  • B. E. Malyugin
  • S. L. Kotova
  • P. S. Timashev
  • S. V. Kostenev
  • B. N. Chichkov
  • V. N. Bagratashvili

External Research Organisations

  • Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics” of the Russian Academy of Sciences
  • S. Fyodorov Eye Microsurgery State Institution
  • Nizhny Novgorod State Medical Acad.
  • RAS - N.N.Semenov Institute of Chemical Physics
  • Sechenov First Moscow State Medical University
  • Laser Zentrum Hannover e.V. (LZH)
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Details

Original languageEnglish
Article number015602
JournalLaser physics letters
Volume15
Issue number1
Publication statusPublished - 13 Dec 2017
Externally publishedYes

Abstract

A two-photon laser femtosecond crosslinking process at the wavelength of 525 nm was studied in a human donor cornea in the presence of riboflavin using two-photon optical microscopy and nanoindentation. It was shown that such an approach results in efficient crosslinking of the corneal collagen and a significant (three-fold) increase in the Young's modulus of the corneal structure. Application of a femtosecond laser with the wavelength of 525 nm allows a drastic enhancement of efficiency in the presence of riboflavin on human corneas and a 50-fold reduction of the laser treatment duration in comparison with the use of a femtosecond laser with the wavelength of 760 nm. We relate this effect to a significant growth in the coefficient of two-photon absorption due to the laser wavelength falling within the edge of the photoinitiator (riboflavin) absorption band. Our studies on a donor human cornea demonstrate the good potential for the clinical application of a femtosecond laser with the wavelength of 525 nm for increasing the cornea rigidity using the two-photon laser femtosecond crosslinking technique.

Keywords

    525 nm femtosecond laser, human cornea, keratoconus, micromechanical properties, riboflavin, two-photon collagen crosslinking

ASJC Scopus subject areas

Cite this

Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. / Shavkuta, B. S.; Gerasimov, M. Y.; Minaev, N. V. et al.
In: Laser physics letters, Vol. 15, No. 1, 015602, 13.12.2017.

Research output: Contribution to journal › Article › Research › peer review

Shavkuta, BS, Gerasimov, MY, Minaev, NV, Kuznetsova, DS, Dudenkova, VV, Mushkova, IA, Malyugin, BE, Kotova, SL, Timashev, PS, Kostenev, SV, Chichkov, BN & Bagratashvili, VN 2017, 'Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea', Laser physics letters, vol. 15, no. 1, 015602. https://doi.org/10.1088/1612-202x/aa963b
Shavkuta, B. S., Gerasimov, M. Y., Minaev, N. V., Kuznetsova, D. S., Dudenkova, V. V., Mushkova, I. A., Malyugin, B. E., Kotova, S. L., Timashev, P. S., Kostenev, S. V., Chichkov, B. N., & Bagratashvili, V. N. (2017). Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. Laser physics letters, 15(1), Article 015602. https://doi.org/10.1088/1612-202x/aa963b
Shavkuta BS, Gerasimov MY, Minaev NV, Kuznetsova DS, Dudenkova VV, Mushkova IA et al. Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. Laser physics letters. 2017 Dec 13;15(1):015602. doi: 10.1088/1612-202x/aa963b
Shavkuta, B. S. ; Gerasimov, M. Y. ; Minaev, N. V. et al. / Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. In: Laser physics letters. 2017 ; Vol. 15, No. 1.
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abstract = "A two-photon laser femtosecond crosslinking process at the wavelength of 525 nm was studied in a human donor cornea in the presence of riboflavin using two-photon optical microscopy and nanoindentation. It was shown that such an approach results in efficient crosslinking of the corneal collagen and a significant (three-fold) increase in the Young's modulus of the corneal structure. Application of a femtosecond laser with the wavelength of 525 nm allows a drastic enhancement of efficiency in the presence of riboflavin on human corneas and a 50-fold reduction of the laser treatment duration in comparison with the use of a femtosecond laser with the wavelength of 760 nm. We relate this effect to a significant growth in the coefficient of two-photon absorption due to the laser wavelength falling within the edge of the photoinitiator (riboflavin) absorption band. Our studies on a donor human cornea demonstrate the good potential for the clinical application of a femtosecond laser with the wavelength of 525 nm for increasing the cornea rigidity using the two-photon laser femtosecond crosslinking technique.",
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AU - Shavkuta, B. S.

AU - Gerasimov, M. Y.

AU - Minaev, N. V.

AU - Kuznetsova, D. S.

AU - Dudenkova, V. V.

AU - Mushkova, I. A.

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AU - Kotova, S. L.

AU - Timashev, P. S.

AU - Kostenev, S. V.

AU - Chichkov, B. N.

AU - Bagratashvili, V. N.

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PY - 2017/12/13

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N2 - A two-photon laser femtosecond crosslinking process at the wavelength of 525 nm was studied in a human donor cornea in the presence of riboflavin using two-photon optical microscopy and nanoindentation. It was shown that such an approach results in efficient crosslinking of the corneal collagen and a significant (three-fold) increase in the Young's modulus of the corneal structure. Application of a femtosecond laser with the wavelength of 525 nm allows a drastic enhancement of efficiency in the presence of riboflavin on human corneas and a 50-fold reduction of the laser treatment duration in comparison with the use of a femtosecond laser with the wavelength of 760 nm. We relate this effect to a significant growth in the coefficient of two-photon absorption due to the laser wavelength falling within the edge of the photoinitiator (riboflavin) absorption band. Our studies on a donor human cornea demonstrate the good potential for the clinical application of a femtosecond laser with the wavelength of 525 nm for increasing the cornea rigidity using the two-photon laser femtosecond crosslinking technique.

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