Details
| Original language | English |
|---|---|
| Pages (from-to) | 4837-4845 |
| Number of pages | 9 |
| Journal | Chemical science |
| Volume | 6 |
| Issue number | 8 |
| Publication status | Published - 1 Aug 2015 |
Abstract
The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
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In: Chemical science, Vol. 6, No. 8, 01.08.2015, p. 4837-4845.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae
AU - Song, Zhongshu
AU - Bakeer, Walid
AU - Marshall, James W.
AU - Yakasai, Ahmed A.
AU - Khalid, Rozida Mohd
AU - Collemare, Jerome
AU - Skellam, Elizabeth
AU - Tharreau, Didier
AU - Lebrun, Marc Henri
AU - Lazarus, Colin M.
AU - Bailey, Andrew M.
AU - Simpson, Thomas J.
AU - Cox, Russell J.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.
AB - The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.
UR - http://www.scopus.com/inward/record.url?scp=84937231692&partnerID=8YFLogxK
U2 - 10.1039/c4sc03707c
DO - 10.1039/c4sc03707c
M3 - Article
AN - SCOPUS:84937231692
VL - 6
SP - 4837
EP - 4845
JO - Chemical science
JF - Chemical science
SN - 2041-6520
IS - 8
ER -